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Kobaylarda vitamin C ve vitamin E uygulamalarının yara iyileşmesi, doku mineral madde ve hidroksiprolin düzeyleri üzerine etkileri

Year 2012, Volume: 28 Issue: 2, 87 - 93, 01.06.2012

Abstract

Amaç: Bu çalışmada E ve C vitaminlerinin kobaylarda yara iyileşmesi ve yara dokusu hidroksiprolin, demir (Fe), bakır (Cu) ve çinko (Zn) düzeyleri üzerine etkilerinin araştırılması amaçlandı.Gereç ve Yöntem: Araştırmada 84 adet kobay, kontrol (Serum fizyolojik, SC), vitamin C (150 mg/kg, SC), vitamin E (150 mg/kg, SC) ve vitamin E + C grupları olmak üzere dört gruba ayrıldı. Yara oluşturulmadan 4 gün önce ve yara oluşturulduktan sonra 1, 4 ve 7. günler vitamin enjeksiyonları gerçekleştirildi. Anesteziye alınan kobayların lumbal omurlarının lateralinde 4 cm uzunluğunda derinin tüm katlarını içerecek şekilde ensizyon yarası oluşturuldu ve basit ayrı dikiş tekniği ile dikilerek yara bölgesi açık havaya bırakıldı. Gruplara enjeksiyonların yapıldığı 4, 7 ile 10. gün her bir gruptan 7’şer kobay ötenazi edilerek, yara bölgesi ensizyonla alındı. Alınan dokularda histo-patolojik muayeneler, doku Fe, Cu, Zn ve hidroksiprolin analizleri gerçekleştirildi.Bulgular: Yara dokusu hidroksiprolin düzeylerinin bütün gruplarda iyileşmenin 7. gününde maksimum düzeylerde olduğu ve Cu ile Zn düzeyleri ile negatif ilişki bulunduğu belirlendi. Yapılan histopatolojik değerlendirmeleri sonucunda, 4. günde, E vitamini grubunda reepitelizasyonun en az olduğu belirlenirken, C vitamini grubunda reepitelizasyonun ve fibroblast aktivitesinin tüm gruplardan daha iyi olduğu görüldü. Çalışmanın 7. gününde ise reepitelizasyon kalitesi açısından en iyi grubun C + E vitamin grubunun olduğu gözlendi. Ensizyon yaralarının iyileşmesi üzerine C vitamininin tek başına ya da C + E vitaminlerinin birlikte kullanımının iyileşmeyi hızlandırıcı etkisinden söz edilebileceği kanısına varıldı.

References

  • Agren MS, Franzen L, 1990. Influence of zinc deficiency on breaking strength of 3-week-old skin incisions in the rat. Acta Chir Scand, 156, 667-670.
  • Arnold HL, Odom RB, James WD, 1990. Andrews’ Diseases of the Skin, Clinical Dermatology, 8th edition, WB Saun- ders Company, Philadelphia, USA.
  • Bates CJ, 1981. The function and metabolism of vitamin C in man, in; Vitamin C (Ascorbic Acid), 1st edition, Eds; Counsell JN, Hornig DH, Applied Science, London, UK, pp:1-22.
  • Başpınar N, Kurtoğlu F, 2003. Vitaminler, S. Ü. Veteriner Fa- kültesi Yayın Ünitesi, Konya, Türkiye.
  • Berg JM, Shi Y, 1996. The galvanization of biology: A gro- wing appreciation for the roles of zinc. Science, 271, 1081-1085.
  • Cook JD, Monsen ER, 1977. Vitamin C: The common cold and iron metabolism. Am J Clin Nutr, 30, 235-241.
  • Dündar Y, Aslan R, 1999. Bir antioksidan olarak vitamin E. Genel Tıp Derg, 9, 109-116.
  • Ehrlich HP, Tarver H, Hunt TK, 1972. Inhibitory effects of vi- tamin E on collagen synthesis and wound repair. Ann Surg, 175, 235-240.
  • Ehrlich HP, Tarver H, Hunt TK, 1973. Effects of vitamin A and glucocorticoids upon inflammation and collagen synthesis. Ann Surg, 177, 222-227.
  • Englard S, Seifter S, 1986. The biochemical functions of as- corbic acid. Annu Rev Nutr, 6, 365-406.
  • Galeano M, Torre V, Deodato B, et al, 2001. Raxofelast, a hydrophilic vitamin E-like antioxidant, stimulates wo- und healing in genetically diabetic mice. Surgery, 129, 467-477.
  • Goode CA, Dinh CT, Linder MC, 1989. Mechanism of copper transport and delivery in mammals: review and recent findings. Adv Exp Med Biol, 258, 131-144.
  • Greenwald DP, Sharzer LA, Padawer J, Levenson SM, Seifter E, 1990. Zone II flexor tendon repair: Effects of vitamins A, E, beta-carotene. J Surg Res, 49, 98-102.
  • Havlik RJ, 1997. Vitamin E and wound healing. Plastic Sur- gery Educational Foundation DATA Committee. Plast Reconstr Surg, 100, 1901-1902.
  • Heng MK, Song MK, Heng MC, 1993. Reciprocity between tissue calmodulin and cAMP levels: modulation by ex- cess zinc. Br J Dermatol, 129, 280-285.
  • Kagoma P, Burger SN, Seifter E, Levenson SM, Demetriou AA, 1985. The effect of vitamin E on experimentally induced peritoneal adhesions in mice. Arch Surg, 120, 949-951.
  • Kaplan B, Gönül B, Dinçer S, Kaya ND, Babül A, 2004. Re- lationships between tensile strength, ascorbic acid, hydroxyproline, and zinc levels of rabbit full-thickness incision wound healing. Surg Today, 34, 747-751.
  • Klevay LM, 1975. Coronary heart disease: the zinc/copper hypothesis, Am J Clin Nutr, 28, 764-774.
  • Lansdown AB, 1995. Physiological and toxicological chan- ges in the skin resulting from the action and interaction of metal ions. Crit Rev Toxicol, 25, 397-462.
  • Lansdown AB, 1996. Zinc in the healing wound. Lancet, 347, 706-707.
  • Lansdown AB, Path FRC, Mirastschijski U, Stubbs N, Scan- lon E, Agren MS, 2007. Zinc in wound healing: Theroeti- cal, experimental, and clinical aspects. Wound Rep Reg, 14, 2-16.
  • Lansdown AB, Sampson B, Rowe A, 1999. Sequential chan- ges in trace metal metallothionein and calmodulin con- centrations in healing skin wounds. J Anat, 195, 375- 386.
  • MacKay DJ and Miller AL, 2003. Nutritional support for wo- und healing. Altern Med Rev, 8, 359-377.
  • Marks R, Dykes P, Motley R, 1993. Clinical Signs and Proce- dures in Dermatology. lst edition, London, UK.
  • Niki E, 1987. Interaction of ascorbate and alpha-tocopherol. Ann N Y Acad Sci, 498, 187-198.
  • Novikova AN, Mamedov LA, Egorova ND, Shekhter AB, Niko- laev AV, 1988. Ascorbic acid and hydroxyproline levels in the serum and granulation tissue of rats with aseptic and infected wounds. Biull Eksp Biol Med, 106, 355-358.
  • Orgill D, Demling RH, 1988. Current concepts and approac- hes to wound healing. Crit Care Med, 16, 899-908.
  • Peterkofsky B, 1991. Ascorbate requirement for hydroxyla- tion and secretion of procollagen: relationship to inhibi- tion of collagen synthesis in scurvy. Am J Clin Nutr, 54, (6 Suppl), 1135-1140.
  • Pinnel SR, Murad S, Darr D, 1987. Induction of collagen synthesis by ascorbic acid. A possible mechanism. Arch Dermatol, 123, 1684-1686.
  • Porto da Rocha, Lucio DP, Suoza Tde L, 2002. Effects of a vi- tamin pool (vitamins A, E, and C) on the tissue necro- sis process: experimental study on rats. Aesthetic Plast Surg, 26, 197-202.
  • Poyraz Ö, 2000. Laboratuvar Hayvanları Bilimi, Kardelen Of- set, Ankara, Türkiye.
  • Prasad AS, 1995. Zinc: An overview. Nutrition, 11, 93-99.
  • Scholl D, Langkamp-Henken B, 2001. Nutrient recommen- dations for wound healing. J Intraven Nurs, 24, 124-132.
  • SPSS 10.0 for Windows. Statistical Package for the Soci- al Sciences. Superior Performines Software System Inc, Chicago, IL, USA.
  • Taren DL, Chvapil M, Weber CW, 1987. Increasing the brea- king strength of wounds exposed to preoperative irradi- ation using vitamin E supplementation. Int J Vitam Nutr Res, 57, 133-137.
  • Woessner JF, 1961. The determination of hyroxyproline in tissue and protein samples. Arch Biochem Biophys, 93, 440-444.
  • Yeoh-Ellerton S, Stacey MC, 2003. Iron and isoprostane le- vels in acute and chronic wounds. J Invest Dermatol, 121, 918-925.

Effects of vitamin C and vitamin E applications on wound healing and tissue mineral and hydroxyprolin levels in guinea pig

Year 2012, Volume: 28 Issue: 2, 87 - 93, 01.06.2012

Abstract

Aim: This study was performed for investigating the effects of vitamin E and vitamin C on guinea pig wound healing and tissue hydroxi-proline, iron (Fe), copper (Cu) and zinc (Zn) levels. Materials and Methods: A totally of 84 guinea pig were divided into four groups; the control group (saline, SC), vitamin C (150 mg/kg, SC), vitamin E (150 mg/kg, SC) and vitamin C + vitamin E groups. Vitamin and saline injections were applied 4 days before and 1, 4 and 7 days after the wound incision. A four centimeters full thickness skin incision wound was created on the lateral lumbar area. The wound area closed with basic separate sutures and leaved open to air. Seven rats from each group were sacrificed on the days of 4, 7 and 10 after the incisions. Wound tissue histopathologic exam was done and tissues hydroxi-proline, Fe, Cu and Zn levels were measured.Results: Tissue hydroxi-proline levels were highest at 7th day in all groups and tissue hydroxi-proline level was negative correlated with Cu and Zn levels. In the histopathologic examination at 4th day of incision, while reepithelisation was lowest in the vitamin E group, reepithelisation and fibroblast activity were highest in the vitamin C group. Reepithelisation was highest on vitamin C + E group at the 7th day of the incision.Conclusion: Vitamin C alone or vitamin C + E administrations may be positively effective on the healing of incisions.

References

  • Agren MS, Franzen L, 1990. Influence of zinc deficiency on breaking strength of 3-week-old skin incisions in the rat. Acta Chir Scand, 156, 667-670.
  • Arnold HL, Odom RB, James WD, 1990. Andrews’ Diseases of the Skin, Clinical Dermatology, 8th edition, WB Saun- ders Company, Philadelphia, USA.
  • Bates CJ, 1981. The function and metabolism of vitamin C in man, in; Vitamin C (Ascorbic Acid), 1st edition, Eds; Counsell JN, Hornig DH, Applied Science, London, UK, pp:1-22.
  • Başpınar N, Kurtoğlu F, 2003. Vitaminler, S. Ü. Veteriner Fa- kültesi Yayın Ünitesi, Konya, Türkiye.
  • Berg JM, Shi Y, 1996. The galvanization of biology: A gro- wing appreciation for the roles of zinc. Science, 271, 1081-1085.
  • Cook JD, Monsen ER, 1977. Vitamin C: The common cold and iron metabolism. Am J Clin Nutr, 30, 235-241.
  • Dündar Y, Aslan R, 1999. Bir antioksidan olarak vitamin E. Genel Tıp Derg, 9, 109-116.
  • Ehrlich HP, Tarver H, Hunt TK, 1972. Inhibitory effects of vi- tamin E on collagen synthesis and wound repair. Ann Surg, 175, 235-240.
  • Ehrlich HP, Tarver H, Hunt TK, 1973. Effects of vitamin A and glucocorticoids upon inflammation and collagen synthesis. Ann Surg, 177, 222-227.
  • Englard S, Seifter S, 1986. The biochemical functions of as- corbic acid. Annu Rev Nutr, 6, 365-406.
  • Galeano M, Torre V, Deodato B, et al, 2001. Raxofelast, a hydrophilic vitamin E-like antioxidant, stimulates wo- und healing in genetically diabetic mice. Surgery, 129, 467-477.
  • Goode CA, Dinh CT, Linder MC, 1989. Mechanism of copper transport and delivery in mammals: review and recent findings. Adv Exp Med Biol, 258, 131-144.
  • Greenwald DP, Sharzer LA, Padawer J, Levenson SM, Seifter E, 1990. Zone II flexor tendon repair: Effects of vitamins A, E, beta-carotene. J Surg Res, 49, 98-102.
  • Havlik RJ, 1997. Vitamin E and wound healing. Plastic Sur- gery Educational Foundation DATA Committee. Plast Reconstr Surg, 100, 1901-1902.
  • Heng MK, Song MK, Heng MC, 1993. Reciprocity between tissue calmodulin and cAMP levels: modulation by ex- cess zinc. Br J Dermatol, 129, 280-285.
  • Kagoma P, Burger SN, Seifter E, Levenson SM, Demetriou AA, 1985. The effect of vitamin E on experimentally induced peritoneal adhesions in mice. Arch Surg, 120, 949-951.
  • Kaplan B, Gönül B, Dinçer S, Kaya ND, Babül A, 2004. Re- lationships between tensile strength, ascorbic acid, hydroxyproline, and zinc levels of rabbit full-thickness incision wound healing. Surg Today, 34, 747-751.
  • Klevay LM, 1975. Coronary heart disease: the zinc/copper hypothesis, Am J Clin Nutr, 28, 764-774.
  • Lansdown AB, 1995. Physiological and toxicological chan- ges in the skin resulting from the action and interaction of metal ions. Crit Rev Toxicol, 25, 397-462.
  • Lansdown AB, 1996. Zinc in the healing wound. Lancet, 347, 706-707.
  • Lansdown AB, Path FRC, Mirastschijski U, Stubbs N, Scan- lon E, Agren MS, 2007. Zinc in wound healing: Theroeti- cal, experimental, and clinical aspects. Wound Rep Reg, 14, 2-16.
  • Lansdown AB, Sampson B, Rowe A, 1999. Sequential chan- ges in trace metal metallothionein and calmodulin con- centrations in healing skin wounds. J Anat, 195, 375- 386.
  • MacKay DJ and Miller AL, 2003. Nutritional support for wo- und healing. Altern Med Rev, 8, 359-377.
  • Marks R, Dykes P, Motley R, 1993. Clinical Signs and Proce- dures in Dermatology. lst edition, London, UK.
  • Niki E, 1987. Interaction of ascorbate and alpha-tocopherol. Ann N Y Acad Sci, 498, 187-198.
  • Novikova AN, Mamedov LA, Egorova ND, Shekhter AB, Niko- laev AV, 1988. Ascorbic acid and hydroxyproline levels in the serum and granulation tissue of rats with aseptic and infected wounds. Biull Eksp Biol Med, 106, 355-358.
  • Orgill D, Demling RH, 1988. Current concepts and approac- hes to wound healing. Crit Care Med, 16, 899-908.
  • Peterkofsky B, 1991. Ascorbate requirement for hydroxyla- tion and secretion of procollagen: relationship to inhibi- tion of collagen synthesis in scurvy. Am J Clin Nutr, 54, (6 Suppl), 1135-1140.
  • Pinnel SR, Murad S, Darr D, 1987. Induction of collagen synthesis by ascorbic acid. A possible mechanism. Arch Dermatol, 123, 1684-1686.
  • Porto da Rocha, Lucio DP, Suoza Tde L, 2002. Effects of a vi- tamin pool (vitamins A, E, and C) on the tissue necro- sis process: experimental study on rats. Aesthetic Plast Surg, 26, 197-202.
  • Poyraz Ö, 2000. Laboratuvar Hayvanları Bilimi, Kardelen Of- set, Ankara, Türkiye.
  • Prasad AS, 1995. Zinc: An overview. Nutrition, 11, 93-99.
  • Scholl D, Langkamp-Henken B, 2001. Nutrient recommen- dations for wound healing. J Intraven Nurs, 24, 124-132.
  • SPSS 10.0 for Windows. Statistical Package for the Soci- al Sciences. Superior Performines Software System Inc, Chicago, IL, USA.
  • Taren DL, Chvapil M, Weber CW, 1987. Increasing the brea- king strength of wounds exposed to preoperative irradi- ation using vitamin E supplementation. Int J Vitam Nutr Res, 57, 133-137.
  • Woessner JF, 1961. The determination of hyroxyproline in tissue and protein samples. Arch Biochem Biophys, 93, 440-444.
  • Yeoh-Ellerton S, Stacey MC, 2003. Iron and isoprostane le- vels in acute and chronic wounds. J Invest Dermatol, 121, 918-925.
There are 37 citations in total.

Details

Other ID JA69KD55DF
Journal Section Research
Authors

Saliha Filiz Pekcici Çuhadar This is me

Seyfullah Haliloğlu This is me

Publication Date June 1, 2012
Published in Issue Year 2012 Volume: 28 Issue: 2

Cite

APA Pekcici Çuhadar, S. F., & Haliloğlu, S. (2012). Effects of vitamin C and vitamin E applications on wound healing and tissue mineral and hydroxyprolin levels in guinea pig. Eurasian Journal of Veterinary Sciences, 28(2), 87-93.
AMA Pekcici Çuhadar SF, Haliloğlu S. Effects of vitamin C and vitamin E applications on wound healing and tissue mineral and hydroxyprolin levels in guinea pig. Eurasian J Vet Sci. June 2012;28(2):87-93.
Chicago Pekcici Çuhadar, Saliha Filiz, and Seyfullah Haliloğlu. “Effects of Vitamin C and Vitamin E Applications on Wound Healing and Tissue Mineral and Hydroxyprolin Levels in Guinea Pig”. Eurasian Journal of Veterinary Sciences 28, no. 2 (June 2012): 87-93.
EndNote Pekcici Çuhadar SF, Haliloğlu S (June 1, 2012) Effects of vitamin C and vitamin E applications on wound healing and tissue mineral and hydroxyprolin levels in guinea pig. Eurasian Journal of Veterinary Sciences 28 2 87–93.
IEEE S. F. Pekcici Çuhadar and S. Haliloğlu, “Effects of vitamin C and vitamin E applications on wound healing and tissue mineral and hydroxyprolin levels in guinea pig”, Eurasian J Vet Sci, vol. 28, no. 2, pp. 87–93, 2012.
ISNAD Pekcici Çuhadar, Saliha Filiz - Haliloğlu, Seyfullah. “Effects of Vitamin C and Vitamin E Applications on Wound Healing and Tissue Mineral and Hydroxyprolin Levels in Guinea Pig”. Eurasian Journal of Veterinary Sciences 28/2 (June 2012), 87-93.
JAMA Pekcici Çuhadar SF, Haliloğlu S. Effects of vitamin C and vitamin E applications on wound healing and tissue mineral and hydroxyprolin levels in guinea pig. Eurasian J Vet Sci. 2012;28:87–93.
MLA Pekcici Çuhadar, Saliha Filiz and Seyfullah Haliloğlu. “Effects of Vitamin C and Vitamin E Applications on Wound Healing and Tissue Mineral and Hydroxyprolin Levels in Guinea Pig”. Eurasian Journal of Veterinary Sciences, vol. 28, no. 2, 2012, pp. 87-93.
Vancouver Pekcici Çuhadar SF, Haliloğlu S. Effects of vitamin C and vitamin E applications on wound healing and tissue mineral and hydroxyprolin levels in guinea pig. Eurasian J Vet Sci. 2012;28(2):87-93.