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Serodiagnosis of Brucella canis infection in dogs by a dipstick enzyme immunoassay

Year 2010, Volume: 26 Issue: 2, 109 - 112, 01.06.2010

Abstract

Aim: A rapid and reliable test is needed by the veterinary practitioners although the canine brucellosis can be diagnosed by bacteriological, molecular and serological methods. By this study, developing a dipstick EIA for a rapid serodiagnosing the infection was aimed. Materials and Methods: The test was compared with Rapid Agglutination Test iELISA.Results: Sensitivity and specificity of the test were 82% and 95%, respectively when iELISA was evaluated as gold standard. Conclusion: Since the test has a good degree of specifity it is recommended as a screening test in the field.

Köpeklerde Brucella canis enfeksiyonunun dipstik enzim immunoassay ile teşhisi

Year 2010, Volume: 26 Issue: 2, 109 - 112, 01.06.2010

Abstract

Amaç: Köpeklerin brusellozunun teşhisi bakteriyolojik, serolojik ve moleküler yöntemlerle yapılmasına rağmen pratisyen veteriner hekimlerin hızlı ve güvenilir bir teste ihtiyaçları vardır. Bu çalışmada hızlı serolojik tanı için Dipstik EIA geliştirilmesi amaçlandı. Gereç ve Yöntem: Test, Çabuk Lam Aglütinasyon ve indirekt ELISA testleri ile karşılaştırıldı. Bulgular: Testin, iELISA’ya göre %82 sensitivite ve %95 spesifiteye sahip olduğu belirlendi. Öneri: Testin yüksek spesifitesinden dolayı saha şartlarında tarama testi olarak kullanımı önerilir.

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Details

Other ID JA69RV73RA
Journal Section Research
Authors

Uckun Sait Ucan This is me

Zeki Aras This is me

Ahmet Semacan This is me

Publication Date June 1, 2010
Published in Issue Year 2010 Volume: 26 Issue: 2

Cite

APA Ucan, U. S., Aras, Z., & Semacan, A. (2010). Serodiagnosis of Brucella canis infection in dogs by a dipstick enzyme immunoassay. Eurasian Journal of Veterinary Sciences, 26(2), 109-112.
AMA Ucan US, Aras Z, Semacan A. Serodiagnosis of Brucella canis infection in dogs by a dipstick enzyme immunoassay. Eurasian J Vet Sci. June 2010;26(2):109-112.
Chicago Ucan, Uckun Sait, Zeki Aras, and Ahmet Semacan. “Serodiagnosis of Brucella Canis Infection in Dogs by a Dipstick Enzyme Immunoassay”. Eurasian Journal of Veterinary Sciences 26, no. 2 (June 2010): 109-12.
EndNote Ucan US, Aras Z, Semacan A (June 1, 2010) Serodiagnosis of Brucella canis infection in dogs by a dipstick enzyme immunoassay. Eurasian Journal of Veterinary Sciences 26 2 109–112.
IEEE U. S. Ucan, Z. Aras, and A. Semacan, “Serodiagnosis of Brucella canis infection in dogs by a dipstick enzyme immunoassay”, Eurasian J Vet Sci, vol. 26, no. 2, pp. 109–112, 2010.
ISNAD Ucan, Uckun Sait et al. “Serodiagnosis of Brucella Canis Infection in Dogs by a Dipstick Enzyme Immunoassay”. Eurasian Journal of Veterinary Sciences 26/2 (June 2010), 109-112.
JAMA Ucan US, Aras Z, Semacan A. Serodiagnosis of Brucella canis infection in dogs by a dipstick enzyme immunoassay. Eurasian J Vet Sci. 2010;26:109–112.
MLA Ucan, Uckun Sait et al. “Serodiagnosis of Brucella Canis Infection in Dogs by a Dipstick Enzyme Immunoassay”. Eurasian Journal of Veterinary Sciences, vol. 26, no. 2, 2010, pp. 109-12.
Vancouver Ucan US, Aras Z, Semacan A. Serodiagnosis of Brucella canis infection in dogs by a dipstick enzyme immunoassay. Eurasian J Vet Sci. 2010;26(2):109-12.