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Hindilerde Ornithobacterium rhinotracheale için aşıların geliştirilmesi ve üretilmesi

Year 2010, Volume: 26 Issue: 2, 101 - 107, 01.06.2010

Abstract

Amaç: Bu çalışmanın amacı bivalan inaktif Ornithobacterium rhinotracheale aşıları hazırlamak, kan serumlarında antijenlere karşı antikorların titrelerini ölçmek ve hindilerde O. rhinotracheale aşılarının etkinliklerini belirlemektir.Gereç ve Yöntem: Bivalan inaktif O. rhinotracheale aşıları; alüminyum hidroksit, mineral yağlı, alüminyum hidroksit + ginseng ve mineral yağ + ginseng adjuvantları kullanılarak O. rhinotracheale serotip A ve B’den hazırlandı. Sterilite ve zararsızlık testlerinden sonra, hindilerde (5. ve 8. haftalarda 0.25ml ve 0.5 ml dozlarla iki kez aşılama ile) aşıların laboratuvar etkinlikleri (çelınç/koruma ve serolojik potens) yapıldı. Bulgular: Çelınç sonuçlarına göre hindilerde bütün aşıların %100 etkili olduğu bulundu. Aşılı ve aşısız grupların serumlarında titrelerin serolojik ölçümleri için ve saha şartlarında O. rhinotracheale enfeksiyonunun teşhisinde lam aglütinasyon, mikro serum aglütinasyon ve ELISA testleri kullanıldı. Adjuvant olarak mineral yağ ve ginseng içeren aşı diğerlerine göre belirgin olarak daha yüksek humoral immune cevap oluşturdu. Aynı zamanda ve mineral yağ + ginseng aşısı özel bir hindi işletmesinde saha denemesinde çok etkili olduğu belirlendi. Öneri: Kanatlılarda ornitobakteriozisin önlenmesi için O. rhinotracheale aşıları kullanılabilir.

Production and development of vaccines for Ornithobacterium rhinotracheale infection in turkeys

Year 2010, Volume: 26 Issue: 2, 101 - 107, 01.06.2010

Abstract

Aim: The purpose of this study was to prepare bivalent inactive Ornithobacterium rhinotracheale bacterin vaccines to measure the levels of antibodies against antigens in blood sera and to determine the efficacies of different O. rhinotracheale vaccines on turkeys. Materials and Methods: The bivalent inactivated O. rhinotracheale bacterin vaccines were prepared from O. rhinotracheale serotype A and B strains using aliminium hydroxide, mineral oil, aluminium hydroxide + ginseng and mineral oil + ginseng. After the sterility and the safety tests, laboratory efficiencies of vaccines (challange/protection and serological potency) were done on the turkeys (twice vaccinated with doses 0.25 ml and 0.5 ml at 5 and 8 weeks, respectively). Results: According to the challenge results, all the vaccines were found effective at 100%. Slide agglutination, micro serum agglutination and ELISA tests were used for the diagnosis. The vaccine containing mineral oil and ginseng as adjuvant induced significantly greater humoral immune response than others. Also, vaccine containing mineral oil and ginseng as adjuvant was determined to be more effective in the field trials in a company privately producing turkeys. Conclusion: O. rhinotracheale vaccines could be used for prevention of ornithobacteriosis in turkeys

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Details

Other ID JA69RP27ZG
Journal Section Research
Authors

Osman Erganis This is me

Hasan Hüseyin Hadimli This is me

Kursat Kav This is me

Zafer Sayin This is me

Zeki Aras This is me

Publication Date June 1, 2010
Published in Issue Year 2010 Volume: 26 Issue: 2

Cite

APA Erganis, O., Hadimli, H. H., Kav, K., Sayin, Z., et al. (2010). Production and development of vaccines for Ornithobacterium rhinotracheale infection in turkeys. Eurasian Journal of Veterinary Sciences, 26(2), 101-107.
AMA Erganis O, Hadimli HH, Kav K, Sayin Z, Aras Z. Production and development of vaccines for Ornithobacterium rhinotracheale infection in turkeys. Eurasian J Vet Sci. June 2010;26(2):101-107.
Chicago Erganis, Osman, Hasan Hüseyin Hadimli, Kursat Kav, Zafer Sayin, and Zeki Aras. “Production and Development of Vaccines for Ornithobacterium Rhinotracheale Infection in Turkeys”. Eurasian Journal of Veterinary Sciences 26, no. 2 (June 2010): 101-7.
EndNote Erganis O, Hadimli HH, Kav K, Sayin Z, Aras Z (June 1, 2010) Production and development of vaccines for Ornithobacterium rhinotracheale infection in turkeys. Eurasian Journal of Veterinary Sciences 26 2 101–107.
IEEE O. Erganis, H. H. Hadimli, K. Kav, Z. Sayin, and Z. Aras, “Production and development of vaccines for Ornithobacterium rhinotracheale infection in turkeys”, Eurasian J Vet Sci, vol. 26, no. 2, pp. 101–107, 2010.
ISNAD Erganis, Osman et al. “Production and Development of Vaccines for Ornithobacterium Rhinotracheale Infection in Turkeys”. Eurasian Journal of Veterinary Sciences 26/2 (June 2010), 101-107.
JAMA Erganis O, Hadimli HH, Kav K, Sayin Z, Aras Z. Production and development of vaccines for Ornithobacterium rhinotracheale infection in turkeys. Eurasian J Vet Sci. 2010;26:101–107.
MLA Erganis, Osman et al. “Production and Development of Vaccines for Ornithobacterium Rhinotracheale Infection in Turkeys”. Eurasian Journal of Veterinary Sciences, vol. 26, no. 2, 2010, pp. 101-7.
Vancouver Erganis O, Hadimli HH, Kav K, Sayin Z, Aras Z. Production and development of vaccines for Ornithobacterium rhinotracheale infection in turkeys. Eurasian J Vet Sci. 2010;26(2):101-7.