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Sığır leptospirozunun patogenezinde ubiquitin ve 8-hidroksiguanozin'in rollerinin immunohistokimyasal yöntemlerle araştırılması

Year 2010, Volume: 26 Issue: 1, 33 - 38, 01.03.2010

Musa Karaman Hasan Özen Serpil Dağ Erginsoy Mitat Şahin

Abstract

Amaç: Bu çalışmada, Leptospira spp. ile doğal enfekte sığırların karaciğer ve böbrek dokularında oluşan hasarda ubiquitin ve 8-hidroksiguanozin’in (8-OHG) rolleri araştırılmıştır. Gereç ve Yöntem: Leptospirozlu (n=7) ve sağlıklı kontrol grubu (n=7) hayvanlarda, bu moleküllere spesifik poliklonal antikorlar kullanılarak indirekt immunoperoksidaz metodu ile antijenlerin dokulardaki lokalizasyonları belirlendi. Bulgular: Leptospirozlu hayvanların karaciğer hepatositleri ve böbrek tubul epitellerinde ubiquitin antikoruna karşı intrasitoplazmik immun boyanma tespit edildi. 8-OHG immun boyanmasının karaciğer hepatositlerinde intrasitoplazmik ve/veya intranükleer, böbrek tubul epitellerinde ise çoğunlukla intrasitoplazmik olarak lokalize olduğu gözlendi. Öneriler: Leptospirozun patogenezinde doku hasarı oluşumunda ubiquitin ile 8-OHG’nin rol oynadığı ortaya konularak, bu moleküllere ilişkin mekanizmaları daha detaylı anlayabilmek için ayrıntılı çalışmaların gerekliliği tavsiye edilmektedir.

Keywords

Leptospiroz ubiquitin 8-hidroksiguanozin patogenez sığır

Immunohistochemical investigation of the role of ubiquitin and 8-hydroxyguanosine in pathogenesis of cattle leptospirosis

Year 2010, Volume: 26 Issue: 1, 33 - 38, 01.03.2010

Musa Karaman Hasan Özen Serpil Dağ Erginsoy Mitat Şahin

Abstract

Aim: This study was performed to investigate the role of ubiquitin and 8-hydroxyguanosine (8-OHG) expression in liver and kidney tissue damage in Leptospira-infected cattle. Material and Method: Localization of these molecules was determined by indirect immunoperoxydase method using specific polyclonal antibodies in Leptospira positive (n=7) and healthy control animals (n=7). Results: A cytoplasmic immunostaining in the hepatocytes and kidney tubule epithelia against ubiquitin antibody was detected in Leptospira-infected animals. Immunostaining against 8-OHG antibody was seen intracytoplasmic and/or intranuclear in the hepatocytes, while it was mostly intracytoplasmic in kidney tubule epithelia. Conclusion: As ubiquitin and 8-OHG were shown to play roles in pathogenesis of Leptospirosis, further detailed studies to investigate and to wholly understand the mode of mechanisms of these molecules are suggested

Keywords

Leptospirosis ubiquitin 8-hydroxyguanosine pathogenesis cattle

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Details

Other ID JA69SF42CU
Journal Section Research
Authors

Musa Karaman This is me

Hasan Özen This is me

Serpil Dağ Erginsoy This is me

Mitat Şahin This is me

Publication Date March 1, 2010
Published in Issue Year 2010 Volume: 26 Issue: 1

Cite

APA Karaman, M., Özen, H., Dağ Erginsoy, S., Şahin, M. (2010). Immunohistochemical investigation of the role of ubiquitin and 8-hydroxyguanosine in pathogenesis of cattle leptospirosis. Eurasian Journal of Veterinary Sciences, 26(1), 33-38.
AMA Karaman M, Özen H, Dağ Erginsoy S, Şahin M. Immunohistochemical investigation of the role of ubiquitin and 8-hydroxyguanosine in pathogenesis of cattle leptospirosis. Eurasian J Vet Sci. March 2010;26(1):33-38.
Chicago Karaman, Musa, Hasan Özen, Serpil Dağ Erginsoy, and Mitat Şahin. “Immunohistochemical Investigation of the Role of Ubiquitin and 8-Hydroxyguanosine in Pathogenesis of Cattle Leptospirosis”. Eurasian Journal of Veterinary Sciences 26, no. 1 (March 2010): 33-38.
EndNote Karaman M, Özen H, Dağ Erginsoy S, Şahin M (March 1, 2010) Immunohistochemical investigation of the role of ubiquitin and 8-hydroxyguanosine in pathogenesis of cattle leptospirosis. Eurasian Journal of Veterinary Sciences 26 1 33–38.
IEEE M. Karaman, H. Özen, S. Dağ Erginsoy, and M. Şahin, “Immunohistochemical investigation of the role of ubiquitin and 8-hydroxyguanosine in pathogenesis of cattle leptospirosis”, Eurasian J Vet Sci, vol. 26, no. 1, pp. 33–38, 2010.
ISNAD Karaman, Musa et al. “Immunohistochemical Investigation of the Role of Ubiquitin and 8-Hydroxyguanosine in Pathogenesis of Cattle Leptospirosis”. Eurasian Journal of Veterinary Sciences 26/1 (March 2010), 33-38.
JAMA Karaman M, Özen H, Dağ Erginsoy S, Şahin M. Immunohistochemical investigation of the role of ubiquitin and 8-hydroxyguanosine in pathogenesis of cattle leptospirosis. Eurasian J Vet Sci. 2010;26:33–38.
MLA Karaman, Musa et al. “Immunohistochemical Investigation of the Role of Ubiquitin and 8-Hydroxyguanosine in Pathogenesis of Cattle Leptospirosis”. Eurasian Journal of Veterinary Sciences, vol. 26, no. 1, 2010, pp. 33-38.
Vancouver Karaman M, Özen H, Dağ Erginsoy S, Şahin M. Immunohistochemical investigation of the role of ubiquitin and 8-hydroxyguanosine in pathogenesis of cattle leptospirosis. Eurasian J Vet Sci. 2010;26(1):33-8.