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SIĞIR MASTİTİSLERİNDEN İZOLE EDİLEN STAFİLOKOK SUŞLARININ BETA-LAKTAMAZ AKTİVİTESİ VE BAZI ANTİBİYOTİKLERE KARŞI DUYARLILIKLARININ SAPTANMASI

Year 2009, Volume: 25 Issue: 1, 23 - 28, 01.03.2009

Abstract

Bu çalışma Kars'ın Sarıkamış ilçesinde, laktasyon periyodunda olan 256 inek üzerinde yürütüldü. Hayvanlar mastitis yönünden
klinik muayeneye tabi tutuldu ve memedeki yangısal değişikliler California Mastitis Testi (eMT) ile belirlendi. Sonuçta, 256 ineğin 61
(%23.8)'inin mastitisli olduğu saptandı. eMT pozitif olduğu belirlenen 61 inekten bakteriyolojik inceleme için 79 süt örneği alındı.
Mikrobiyolojik inceleme sonucunda bu örneklerin 67 (%84.8)'sinde etken izole edilirken, 12 (%15.2)'sinde herhangi bir bakteriyel etken
izolasyonu yapılamadı. Staphylococcus aureus, %34.3 oranı ile en sık saptanan bakteri olurken, bunu %28.3 oranı ile koagülaz negatif
stafilokoklar (KNS) izledi. Bu iki grup mikroorganizma dışında örneklerin %16.4'ünde Streptococcus spp. ve %5.9'unda Escherchia coli
izole edildi. izole edilen stafilokok suşlarından 14 (%33.3) S.aureus ve 4 (%9.5) KNS suşun ß-laktarnaz pozitif olduğu bulundu.
stafilokok suşlarının antibiyotik dirençlilikleri; %59.5'i penisiline, %38.1'i amoksisiline, %7.1'i amoksisilin-klavulonik aside, %4.8'i
kloksasiline, %11.9'u enrofloksasine dirençli ve suşların tamamının vankomisine duyarlı belirlendi. Suşların 13-laktamaz aktivitesinin 13-
laktam grubu antibiyotiklere karşı dirençte önemli bir roloynadığı tespit edildi.

Determining of f3-lactamase Activity and Resistanee Against Some Antibiotics of Staphyloeoeeus Strains Isolated From Bovine Mastilis

Year 2009, Volume: 25 Issue: 1, 23 - 28, 01.03.2009

Abstract

This study was carried out in 256 cows which were in lactation period in Sarıkamış town of Kars. The animals were
examined c1inically in the point of mastitis and inflamational changes of udders were determined with California Mastitis Test (CMT).
Consequently, mastitis were diagnosed in 61 of 256 cows (23.8%). 79 milk sample were taken from 61 cows which were positive in
CMT test. As a result of microbiological examination, while agent was isolated in 67 of all samples (84.8%), no bacterial agent was not
isolated in 12 samples (15.2%). While Staphylococcus aureus was the most determined bacterium with the percentage of 34.3%,
coagulase negative staphylococcus (KNS) followed that whit the persentage of 28.3%. Except these two groups of microorganism,
Escherichia coliwas isolated in the 5.9% and Streptococcus spp. were isolated in the 16.4% of the samples. In 14 (33.3%) S. aureus
and 4 ( 9.5%) KNS strains, of all isolated staphylococcus strains, ß-lacternase was found positive. In the study the resistance of
staphylococcus strains against antibiotics were determined as follows; 59.5% penicillin, 38.1% amoxicillin, 7.1% amoxicillin-c1avulonic
acid, 4.8% c1oxacillin, 11.9% enrofloxacin and all strains were found sensitiye against vancomicin. The ô-lactarnase activity of the
strains was determined to have an important role in resistance against ô-lactarn group antibiotics.

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Details

Other ID JA69SP82YY
Journal Section Research
Authors

Durmuş Ali Sevinti This is me

Mitat Şahin This is me

Publication Date March 1, 2009
Published in Issue Year 2009 Volume: 25 Issue: 1

Cite

APA Sevinti, D. A., & Şahin, M. (2009). Determining of f3-lactamase Activity and Resistanee Against Some Antibiotics of Staphyloeoeeus Strains Isolated From Bovine Mastilis. Eurasian Journal of Veterinary Sciences, 25(1), 23-28.
AMA Sevinti DA, Şahin M. Determining of f3-lactamase Activity and Resistanee Against Some Antibiotics of Staphyloeoeeus Strains Isolated From Bovine Mastilis. Eurasian J Vet Sci. March 2009;25(1):23-28.
Chicago Sevinti, Durmuş Ali, and Mitat Şahin. “Determining of F3-Lactamase Activity and Resistanee Against Some Antibiotics of Staphyloeoeeus Strains Isolated From Bovine Mastilis”. Eurasian Journal of Veterinary Sciences 25, no. 1 (March 2009): 23-28.
EndNote Sevinti DA, Şahin M (March 1, 2009) Determining of f3-lactamase Activity and Resistanee Against Some Antibiotics of Staphyloeoeeus Strains Isolated From Bovine Mastilis. Eurasian Journal of Veterinary Sciences 25 1 23–28.
IEEE D. A. Sevinti and M. Şahin, “Determining of f3-lactamase Activity and Resistanee Against Some Antibiotics of Staphyloeoeeus Strains Isolated From Bovine Mastilis”, Eurasian J Vet Sci, vol. 25, no. 1, pp. 23–28, 2009.
ISNAD Sevinti, Durmuş Ali - Şahin, Mitat. “Determining of F3-Lactamase Activity and Resistanee Against Some Antibiotics of Staphyloeoeeus Strains Isolated From Bovine Mastilis”. Eurasian Journal of Veterinary Sciences 25/1 (March 2009), 23-28.
JAMA Sevinti DA, Şahin M. Determining of f3-lactamase Activity and Resistanee Against Some Antibiotics of Staphyloeoeeus Strains Isolated From Bovine Mastilis. Eurasian J Vet Sci. 2009;25:23–28.
MLA Sevinti, Durmuş Ali and Mitat Şahin. “Determining of F3-Lactamase Activity and Resistanee Against Some Antibiotics of Staphyloeoeeus Strains Isolated From Bovine Mastilis”. Eurasian Journal of Veterinary Sciences, vol. 25, no. 1, 2009, pp. 23-28.
Vancouver Sevinti DA, Şahin M. Determining of f3-lactamase Activity and Resistanee Against Some Antibiotics of Staphyloeoeeus Strains Isolated From Bovine Mastilis. Eurasian J Vet Sci. 2009;25(1):23-8.