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Atların periferal kan mononükler hücrelerinde (PKMH) erken gebelik ve östrus siklusu sürecinde Toll-like reseptörlerinin (TLRs) ekspresyonu

Year 2012, Volume: 28 Issue: 1, 15 - 20, 01.03.2012

Ercan Kurar Mehmet Osman Atli Aydin Guzeloglu Seyit Ali Kayis Ahmet Semacan

Abstract

Amaç: Günümüzde kısraklarda birçok gebelik teşhis metodu olmasına rağmen, mümkün olabilecek en erken zamanda gebeliğinin teşhis edilmesi için pratik, güvenilir ve ekonomik bir yönteme halen ihtiyaç bulunmaktadır. Allojenik fötusa karşı immun tolerans erken gebelik esnasında kritik bir öneme sahiptir. Toll-like reseptörlerinin (TLRs) doğal immun sistemin önemli bir parçası olduğu bilinmektedir. Sunulan çalışmada, kısraklarda erken gebelikte periferal kan mononükler hücrelerinde (PKMH) TLRs ekspresyonunun incelenmesi amaçlandı.Gereç ve Yöntem: Kan numuneleri aynı üç kısraktan östrus siklusunun ve gebeliğin 0 ve 8. günlerinde toplandı. Total RNA örnekleri PKMH’lerinden izole edildi ve cDNA sentezlendi. mRNA düzeyindeki TLRs ekspresyonları iki defa realtime PZR kullanılarak kuantifiye edildi. İstatistiksel analiz için Relative Expression Software Tool (REST2009) kullanıldı. Bulgular: RT-PZR ile PKMH’lerde TLRs ekspresyonları tespit edildi. Bununla beraber, real-time PZR ölçümleri siklik veya gebelik durumunun TLRs ekspresyonu üzerine etkisini göstermekte başarısız kaldı. Öneri: Bu sonuçlara bakıldığında, atlarda gebeliğin erken döneminde PKMH’lerinde TLR genlerinin ekspresyonlarının gebeliğin erken anlaşılması için iyi bir markör olmadığı söylenebilir.

Keywords

TLRs PKMH at gebelik

Expression of Toll-like receptors (TLRs) in the equine peripheral blood mononuclear cells (PBMCs) during the early pregnancy and estrous cycle

Year 2012, Volume: 28 Issue: 1, 15 - 20, 01.03.2012

Ercan Kurar Mehmet Osman Atli Aydin Guzeloglu Seyit Ali Kayis Ahmet Semacan

Abstract

Aim: Practical, reliable and economical pregnancy diagnosis method for as early as possible time of pregnancy in mares is still requirement. Immune tolerance against allogenic fetus is critically important during the early pregnancy. Tolllike Receptors (TLRs) are known to be an important part of innate immune system. Aim was to evaluate expression of TLRs in peripheral blood mononuclear cells (PBMCs) during the early pregnancy in mare.Material and Methods: Blood samples were collected on days of ovulation (d0) and 8 during estrous and pregnancy from three same mares. Total RNA samples were isolated from PBMCs and cDNA synthesis was performed. Expressions of TLRs at mRNA levels were quantified by using realtime RT-PCR in duplicates. Relative Expression Software Tool (REST2009) was used for statistical analysis. Results: TLRs (1 to 10) were found to be expressed in PMBCs by RT-PCR. However, real-time PCR measurements failed to show effects of pregnant or cyclic status on expression of TLRs.Conclusion: According to these results, it may suggest that determination of TLRs gene expression in equine PBMCs during early pregnancy is not a good marker in understanding early equine pregnancy.

Keywords

TLRs PBMCs equine pregnancy

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Details

Other ID JA69KY67PK
Journal Section Research
Authors

Ercan Kurar This is me

Mehmet Osman Atli This is me

Aydin Guzeloglu This is me

Seyit Ali Kayis This is me

Ahmet Semacan This is me

Publication Date March 1, 2012
Published in Issue Year 2012 Volume: 28 Issue: 1

Cite

APA Kurar, E., Atli, M. O., Guzeloglu, A., Kayis, S. A., et al. (2012). Expression of Toll-like receptors (TLRs) in the equine peripheral blood mononuclear cells (PBMCs) during the early pregnancy and estrous cycle. Eurasian Journal of Veterinary Sciences, 28(1), 15-20.
AMA Kurar E, Atli MO, Guzeloglu A, Kayis SA, Semacan A. Expression of Toll-like receptors (TLRs) in the equine peripheral blood mononuclear cells (PBMCs) during the early pregnancy and estrous cycle. Eurasian J Vet Sci. March 2012;28(1):15-20.
Chicago Kurar, Ercan, Mehmet Osman Atli, Aydin Guzeloglu, Seyit Ali Kayis, and Ahmet Semacan. “Expression of Toll-Like Receptors (TLRs) in the Equine Peripheral Blood Mononuclear Cells (PBMCs) During the Early Pregnancy and Estrous Cycle”. Eurasian Journal of Veterinary Sciences 28, no. 1 (March 2012): 15-20.
EndNote Kurar E, Atli MO, Guzeloglu A, Kayis SA, Semacan A (March 1, 2012) Expression of Toll-like receptors (TLRs) in the equine peripheral blood mononuclear cells (PBMCs) during the early pregnancy and estrous cycle. Eurasian Journal of Veterinary Sciences 28 1 15–20.
IEEE E. Kurar, M. O. Atli, A. Guzeloglu, S. A. Kayis, and A. Semacan, “Expression of Toll-like receptors (TLRs) in the equine peripheral blood mononuclear cells (PBMCs) during the early pregnancy and estrous cycle”, Eurasian J Vet Sci, vol. 28, no. 1, pp. 15–20, 2012.
ISNAD Kurar, Ercan et al. “Expression of Toll-Like Receptors (TLRs) in the Equine Peripheral Blood Mononuclear Cells (PBMCs) During the Early Pregnancy and Estrous Cycle”. Eurasian Journal of Veterinary Sciences 28/1 (March 2012), 15-20.
JAMA Kurar E, Atli MO, Guzeloglu A, Kayis SA, Semacan A. Expression of Toll-like receptors (TLRs) in the equine peripheral blood mononuclear cells (PBMCs) during the early pregnancy and estrous cycle. Eurasian J Vet Sci. 2012;28:15–20.
MLA Kurar, Ercan et al. “Expression of Toll-Like Receptors (TLRs) in the Equine Peripheral Blood Mononuclear Cells (PBMCs) During the Early Pregnancy and Estrous Cycle”. Eurasian Journal of Veterinary Sciences, vol. 28, no. 1, 2012, pp. 15-20.
Vancouver Kurar E, Atli MO, Guzeloglu A, Kayis SA, Semacan A. Expression of Toll-like receptors (TLRs) in the equine peripheral blood mononuclear cells (PBMCs) during the early pregnancy and estrous cycle. Eurasian J Vet Sci. 2012;28(1):15-20.