Research Article
BibTex RIS Cite
Year 2016, Volume: 2 Issue: 2, 126 - 131, 04.07.2016
https://doi.org/10.18621/eurj.2016.5000190265

Abstract

References

  • Rahal A, Kumar A, Singh V, Yadav B, Tiwari R, Chakraborty S, et al. Oxidative stress, prooxidants, and antioxidants: the interplay. Biomed Res Int 2014;2014:761264.
  • Lubrano V, Balzan S. Enzymatic antioxidant system in vascular inflammation and coronary artery disease. World J Exp Med 2015;5:218-24.
  • Liu T, Zhong S, Liao X, Chen J, He T, Lai S, et al. A Meta-analysis of oxidative stress markers in depression. PLoS One 2015;10:e0138904.
  • Rysz J, Stolarek RA, Pedzik A, Nowicki M, Nowak D. Serum antioxidant capacity is preserved in peritoneal dialysis contrary to its robust depletion after hemodialysis and hemodiafiltration sessions. Ther Apher Dial 2010;14:209-17.
  • Jansen EH, Ruskovska T. Comparative analysis of serum (anti)oxidative status parаmeters in healthy persons. Int J Mol Sci 2013;14:6106-15.
  • Woudberg NJ, Goedecke JH, Blackhurst D, Frias M, James R, Opie LH, et al. Association between ethnicity and obesity with high-density lipoprotein (HDL) function and subclass distribution. Lipids Health Dis 2016;15:92.
  • Bortolasci CC, Maes M, Vargas HO, Souza-Nogueira A, Moreira EG, Nunes SO, et al. Paraoxonase 1 status and interactions between Q192R functional genotypes by smoking contribute significantly to total plasma radical trapping antioxidant potential. Neurosci Lett 2014;581:46-51.
  • Benzie IF, Strain JJ. The ferric reducing ability of plasma (FRAP) as a measure of ‘‘antioxidant power’’: the FRAP assay. Anal Biochem 1996;239:70-6.
  • Lim CSH, Lim SL. Ferric reducing capacity versus ferric reducing antioxidant power for measuring total antioxidant capacity. Lab Med 2013;44:51-5.
  • Mistry HD, Gill CA, Kurlak LO, Seed PT, Hesketh JE, Meplan C, et al. Association between maternal micronutrient status, oxidative stress, and common genetic variants in antioxidant enzymes at 15 weeks׳ gestation in nulliparous women who subsequently develop preeclampsia. Free Radic Biol Med 2015;78:147-55.
  • Adjei-Fremah S, Jackai LEN, Worku M. Analysis of phenolic content and antioxidant properties of selected cowpea varieties tested in bovine peripheral blood. Am J Animal Vet Sci 2015;10:235-45.
  • Topic E, Nikolac N, Panteghini M, Theodorsson E, Salvagno GL, Miler M, et al. How to assess the quality of your analytical method? Clin Chem Lab Med 2015;53:1707-18.
  • Shrivastava A, Gupta VB. Methods for the determination of limit of detection and limit of quantitation of the analytical methods. Chron Young Sci 2011;2:21-5.
  • Guidi GC, Salvagno GL. Reference intervals as a tool for total quality management. Biochem Med 2010;20:165-72.
  • Korkmaz GG, Konukoglu D, Kurtulus EM, Irmak H, Bolayirli M, Uzun H. Total antioxidant status and markers of oxidative stress in subjects with normal or impaired glucose regulation (IFG, IGT) in diabetic patients. Scand J Clin Lab Invest 2013;73:641-9.
  • Zargari M, Sedighi O. Influence of hemodialysis on lipid peroxidation, enzymatic and non-enzymatic antioxidant capacity in chronic renal failure patients. Nephrourol Mon 2015;7:e28526.
  • Ridefelt P, Hellberg D, Aldrimer M, Gustafsson J. Estimating reliable paediatric reference intervals in clinical chemistry and haematology. Acta Paediatr 2014;103:10-5.
  • Allen MJ, Powers ML, Gronowski KS, Gronowski AM. Human tissue ownership and use in research: what laboratorians and researchers should know. Clin Chem 2010;56:1675-82.
  • Karajibani M, Hashemi M, Montazerifar F, Bolouri A, Dikshit M. The status of glutathione peroxidase, superoxide dismutase, vitamins A, C, E and malondialdehyde in patients with cardiovascular disease in Zahedan, Southeast Iran. J Nutr Sci Vitaminol (Tokyo) 2009;55:309-16.
  • Kumar A, Mishra AK, Rana A, Yadav P. Correlation between oxidative stress and serum iron level in pregnant anemic women in north Indian population. GJRA 2015;4:325-27.
  • Fisher G, Alvarez JA, Ellis AC, Granger WM, Ovalle F, Man CD, et al. Race differences in the association of oxidative stress with insulin in African- and European-American women. Obesity (Silver Spring) 2012;20:972-7.
  • Carrion-Garcia CJ, Guerra-Hernandez EJ, Garcia-Villanova B, Molina-Montes E. Non-enzymatic antioxidant capacity (NEAC) estimated by two different dietary assessment methods and its relationship with NEAC plasma levels. Eur J Nutr 2016.
  • Zamora-Ros R, Serafini M, Estruch R, Lamuela-Raventos RM, Martínez-Gonzalez MA, Salas-Salvado J, et al; PREDIMED Study Investigators. Mediterranean diet and non enzymatic antioxidant capacity in the PREDIMED study: evidence for a mechanism of antioxidant tuning. Nutr Metab Cardiovasc Dis 2013;23:1167-74.
  • Gupta S, Gambhir JK, Kalra O, Gautam A, Shukla K, Mehndiratta M, et al. Association of biomarkers of inflammation and oxidative stress with the risk of chronic kidney disease in type 2 diabetes mellitus in North Indian population. J Diabetes Complications 2013;27:548-52.
  • Patel V, Kanani D, Chauhan K, Haridas N, Makadia M, Patel V. Assessment of oxidative stres in type 2 diabetes mellitus by measurement of plasma lipid hydroperoxides and total antioxidant capacity. Int J Adv Res 2015;3:1078-84.
  • Gopinathan V, Miller NJ, Milner AD, Rice-Evans CA. Bilirubin and ascorbate antioxidant activity in neonatal plasma. FEBS Lett 1994; 1;349:197-200.
  • Dogan M, Peker E, Kirimi E, Sal E, Akbayram S, Erel O, et al. Evaluation of oxidant and antioxidant status in infants with hyperbilirubinemia and kernicterus. Hum Exp Toxicol 2011;30:1751-60.
  • Liao SL. The role of bilirubin and phototherapy in the oxidative/antioxidant balance. Pediatr Neonatol 2015;56:77-8.

Establishing reference values and evaluation of an in-house ferric reducing antioxidant power (FRAP) colorimetric assay in microplates

Year 2016, Volume: 2 Issue: 2, 126 - 131, 04.07.2016
https://doi.org/10.18621/eurj.2016.5000190265

Abstract

Objective. The total antioxidant capacity (TAC) of a sample can be measured with a ferric reducing antioxidant power (FRAP) assay. There are commercially available kits for FRAP assays, however they are more expensive than in-house kits. We aimed to evaluate a FRAP direct measurement method under our laboratory conditions using a microplate reader and establish reference values to use in future research projects. Methods. An in-house microplate adaptation of the FRAP method was evaluated. Reference values of FRAP were established for one hundred and twenty subjects aged between 25-55 years. FRAP levels were estimated in 30 serum samples with high glucose concentration, 44 hyperbiluribinemic neonatals and 16 patients receiving renal replacement therapy (RRT). Results. The mean FRAP level was 890±235 µmol/L. The median TAC level was 904 µmol/L. This method was found to be linear up to at least 2000 µmol/L. The intra- and inter-assay coefficients of variation were 2.7-6.7% and 5.3-10.1%, respectively. The mean FRAP level was lower than normal in diabetes and RRT patients and higher in hyperbiluribinemic neonatals (687±209 µmol/L, 609±250 µmol/L and 945±187 µmol/L, respectively). Conclusions. Our reference values give comparable results with the literature. This method is simple, reliable, and inexpensive. It could be used for studies of oxidative stress-related diseases.

References

  • Rahal A, Kumar A, Singh V, Yadav B, Tiwari R, Chakraborty S, et al. Oxidative stress, prooxidants, and antioxidants: the interplay. Biomed Res Int 2014;2014:761264.
  • Lubrano V, Balzan S. Enzymatic antioxidant system in vascular inflammation and coronary artery disease. World J Exp Med 2015;5:218-24.
  • Liu T, Zhong S, Liao X, Chen J, He T, Lai S, et al. A Meta-analysis of oxidative stress markers in depression. PLoS One 2015;10:e0138904.
  • Rysz J, Stolarek RA, Pedzik A, Nowicki M, Nowak D. Serum antioxidant capacity is preserved in peritoneal dialysis contrary to its robust depletion after hemodialysis and hemodiafiltration sessions. Ther Apher Dial 2010;14:209-17.
  • Jansen EH, Ruskovska T. Comparative analysis of serum (anti)oxidative status parаmeters in healthy persons. Int J Mol Sci 2013;14:6106-15.
  • Woudberg NJ, Goedecke JH, Blackhurst D, Frias M, James R, Opie LH, et al. Association between ethnicity and obesity with high-density lipoprotein (HDL) function and subclass distribution. Lipids Health Dis 2016;15:92.
  • Bortolasci CC, Maes M, Vargas HO, Souza-Nogueira A, Moreira EG, Nunes SO, et al. Paraoxonase 1 status and interactions between Q192R functional genotypes by smoking contribute significantly to total plasma radical trapping antioxidant potential. Neurosci Lett 2014;581:46-51.
  • Benzie IF, Strain JJ. The ferric reducing ability of plasma (FRAP) as a measure of ‘‘antioxidant power’’: the FRAP assay. Anal Biochem 1996;239:70-6.
  • Lim CSH, Lim SL. Ferric reducing capacity versus ferric reducing antioxidant power for measuring total antioxidant capacity. Lab Med 2013;44:51-5.
  • Mistry HD, Gill CA, Kurlak LO, Seed PT, Hesketh JE, Meplan C, et al. Association between maternal micronutrient status, oxidative stress, and common genetic variants in antioxidant enzymes at 15 weeks׳ gestation in nulliparous women who subsequently develop preeclampsia. Free Radic Biol Med 2015;78:147-55.
  • Adjei-Fremah S, Jackai LEN, Worku M. Analysis of phenolic content and antioxidant properties of selected cowpea varieties tested in bovine peripheral blood. Am J Animal Vet Sci 2015;10:235-45.
  • Topic E, Nikolac N, Panteghini M, Theodorsson E, Salvagno GL, Miler M, et al. How to assess the quality of your analytical method? Clin Chem Lab Med 2015;53:1707-18.
  • Shrivastava A, Gupta VB. Methods for the determination of limit of detection and limit of quantitation of the analytical methods. Chron Young Sci 2011;2:21-5.
  • Guidi GC, Salvagno GL. Reference intervals as a tool for total quality management. Biochem Med 2010;20:165-72.
  • Korkmaz GG, Konukoglu D, Kurtulus EM, Irmak H, Bolayirli M, Uzun H. Total antioxidant status and markers of oxidative stress in subjects with normal or impaired glucose regulation (IFG, IGT) in diabetic patients. Scand J Clin Lab Invest 2013;73:641-9.
  • Zargari M, Sedighi O. Influence of hemodialysis on lipid peroxidation, enzymatic and non-enzymatic antioxidant capacity in chronic renal failure patients. Nephrourol Mon 2015;7:e28526.
  • Ridefelt P, Hellberg D, Aldrimer M, Gustafsson J. Estimating reliable paediatric reference intervals in clinical chemistry and haematology. Acta Paediatr 2014;103:10-5.
  • Allen MJ, Powers ML, Gronowski KS, Gronowski AM. Human tissue ownership and use in research: what laboratorians and researchers should know. Clin Chem 2010;56:1675-82.
  • Karajibani M, Hashemi M, Montazerifar F, Bolouri A, Dikshit M. The status of glutathione peroxidase, superoxide dismutase, vitamins A, C, E and malondialdehyde in patients with cardiovascular disease in Zahedan, Southeast Iran. J Nutr Sci Vitaminol (Tokyo) 2009;55:309-16.
  • Kumar A, Mishra AK, Rana A, Yadav P. Correlation between oxidative stress and serum iron level in pregnant anemic women in north Indian population. GJRA 2015;4:325-27.
  • Fisher G, Alvarez JA, Ellis AC, Granger WM, Ovalle F, Man CD, et al. Race differences in the association of oxidative stress with insulin in African- and European-American women. Obesity (Silver Spring) 2012;20:972-7.
  • Carrion-Garcia CJ, Guerra-Hernandez EJ, Garcia-Villanova B, Molina-Montes E. Non-enzymatic antioxidant capacity (NEAC) estimated by two different dietary assessment methods and its relationship with NEAC plasma levels. Eur J Nutr 2016.
  • Zamora-Ros R, Serafini M, Estruch R, Lamuela-Raventos RM, Martínez-Gonzalez MA, Salas-Salvado J, et al; PREDIMED Study Investigators. Mediterranean diet and non enzymatic antioxidant capacity in the PREDIMED study: evidence for a mechanism of antioxidant tuning. Nutr Metab Cardiovasc Dis 2013;23:1167-74.
  • Gupta S, Gambhir JK, Kalra O, Gautam A, Shukla K, Mehndiratta M, et al. Association of biomarkers of inflammation and oxidative stress with the risk of chronic kidney disease in type 2 diabetes mellitus in North Indian population. J Diabetes Complications 2013;27:548-52.
  • Patel V, Kanani D, Chauhan K, Haridas N, Makadia M, Patel V. Assessment of oxidative stres in type 2 diabetes mellitus by measurement of plasma lipid hydroperoxides and total antioxidant capacity. Int J Adv Res 2015;3:1078-84.
  • Gopinathan V, Miller NJ, Milner AD, Rice-Evans CA. Bilirubin and ascorbate antioxidant activity in neonatal plasma. FEBS Lett 1994; 1;349:197-200.
  • Dogan M, Peker E, Kirimi E, Sal E, Akbayram S, Erel O, et al. Evaluation of oxidant and antioxidant status in infants with hyperbilirubinemia and kernicterus. Hum Exp Toxicol 2011;30:1751-60.
  • Liao SL. The role of bilirubin and phototherapy in the oxidative/antioxidant balance. Pediatr Neonatol 2015;56:77-8.
There are 28 citations in total.

Details

Primary Language English
Subjects Biochemistry and Cell Biology (Other)
Journal Section Original Articles
Authors

Yasemin Ustundag

Kagan Huysal This is me

Serdar Kahvecioglu

Hakan Demirci

Senol Yavuz 0000-0001-5246-0808

Murat Sambel

Dursun Unal This is me

Publication Date July 4, 2016
Submission Date May 24, 2016
Acceptance Date June 20, 2016
Published in Issue Year 2016 Volume: 2 Issue: 2

Cite

AMA Ustundag Y, Huysal K, Kahvecioglu S, Demirci H, Yavuz S, Sambel M, Unal D. Establishing reference values and evaluation of an in-house ferric reducing antioxidant power (FRAP) colorimetric assay in microplates. Eur Res J. July 2016;2(2):126-131. doi:10.18621/eurj.2016.5000190265

e-ISSN: 2149-3189 


The European Research Journal, hosted by Turkish JournalPark ACADEMIC, is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

by-nc-nd.png

2024