Integrin binding peptide promotes in vitro wound closure in the L929 mouse fibroblasts

Year 2017, Volume: 3 Issue: 3, 207 - 213, 04.11.2017

Ozan Karaman Günnur Onak Emine Afra Demirci Emine Kahraman

https://doi.org/10.18621/eurj.318606

Cited By: 1

Abstract

Objective. Molecular basis of wound healing process needs to
further examined to determine the effective individual biological cues. The
objective of this study was to investigate the wound closure, proliferation,
and viability of L929 fibroblast when cultured with different concentration of
soluble RGD peptid. Methods. RGD peptide was synthesized manually on solid phase. The
percentage of healed wound area for control, 0.5 mM, 1 mM, and 2 mM at each
time points were analyzed by ImageJ. Cell proliferation and viability were
assessed with MTT and live/ dead analysis, respectively. Results. The results of
wound closure area showed that increased RGD peptide concentration in the
culture improved cellular migration which enables significantly accelerated
wound closure. However, RGD peptide did not dramatically augmented cell
proliferation. In addition, cell viability results indicated that dead cell
numbers did not critically influence by increasing the RGD peptide
concentration in the culture. Conclusions. The present study
showed that soluble integrin binding peptide accelerated the migration and
wound closure rate of L929 fibroblasts. Delivery of soluble integrin binding
peptides into the wound area may be considered as an alternative wound
treatment technique in the near future after proofing the concept study with
animal and clinical studies.

Keywords

Integrin binding peptide, in vitro scratch assay, cell migration, serum free media

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