Research Article
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Year 2019, Volume: 5 Issue: 2, 299 - 305, 04.03.2019
https://doi.org/10.18621/eurj.373385

Abstract

References

  • [1] Sophia Fox AJ, Bedi A, Rodeo SA. The basic science of articular cartilage: structure, composition, and function. Sports Health 2009;1:461-8.
  • [2] Buma P, Pieper JS, Van Tienen T, Van Susante JL, Van der Kraan PM, Veerkamp JH, et al. Cross-linked type I and type II collagenous matrices for the repair of full-thickness articular cartilage defects: a study in rabbits. Biomaterials 2003;24:3255-63.
  • [3] Dohan Ehrenfest DM, Rasmusson L, Albrektsson T. Classification of platelet concentrates: from pure platelet-rich plasma (P-PRP) to leucocyte- and platelet-rich fibrin (L-PRF). Trends Biotechnol 2009;27:158-67.
  • [4] Choukroun J, Adda F, Schoeffler C, Vervelle A. [An opportunity in perio-implantology: the PRF]. Implantodontie 2001;42:55-62. [Article in French]
  • [5] Choukroun J, Diss A, Simonpieri A, Girard MO, Schoeffler C, Dohan SL, et al. Platelet-rich fibrin (PRF): A second-generation platelet concentrate. Part IV: Clinical effects on tissue healing. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2006;101:e56-60.
  • [6] Choukroun J, Diss A, Simonpieri A, Girard MO, Schoeffler C, Dohan SL, et al. Platelet-rich fibrin (PRF): a second-generation platelet concentrate. Part V: Histologic evaluations of PRF effects on bone allograft maturation in sinus lift. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2006;101:299-303.
  • [7] Dohan DM, Choukroun J, Diss A, Dohan SL, Dohan AJ, Mouhyi J, et al. Platelet-rich fibrin (PRF): a second-generation platelet concentrate. Part II: Platelet-related biologic features. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2006;101:e45-50.
  • [8] Dohan DM, Choukroun J, Diss A, Dohan SL, Dohan AJ, Mouhyi J, et al. Platelet-rich fibrin (PRF): a second-generation platelet concentrate. Part I: Technological concepts and evolution. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2006;101:e37-44.
  • [9] Masuki H, Okudera T, Watanebe T, Suzuki M, Nishiyama K, Okudera H, et al. Growth factor and pro-inflammatory cytokine contents in platelet-rich plasma (PRP), plasma rich in growth factors (PRGF), advanced platelet-rich fibrin (A-PRF), and concentrated growth factors (CGF). Int J Implant Dent 2016;2:19.
  • [10] He L, Lin Y, Hu X, Zhang Y, Wu H. A comparative study of platelet-rich fibrin (PRF) and platelet-rich plasma (PRP) on the effect of proliferation and differentiation of rat osteoblasts in vitro. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2009;108:707-13.
  • [11] O'Driscoll SW, Keeley FW, Salter RB. The chondrogenic potential of free autogenous periosteal grafts for biological resurfacing of major full-thickness defects in joint surfaces under the influence of continuous passive motion. An experimental investigation in the rabbit. J Bone Joint Surg Am 1986;68:1017-35.
  • [12] Stone KR, Walgenbach AW, Freyer A, Turek TJ, Speer DP. Articular cartilage paste grafting to full-thickness articular cartilage knee joint lesions: a 2- to 12- year follow-up. Arthroscopy 2006;22:291-9.
  • [13] Su CY, Kuo YP, Tseng YH, Su CH, Burnouf T. In vitro release of growth factors from platelet-rich fibrin (PRF): a proposal to optimize the clinical applications of PRF. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2009;108:56-61.
  • [14] Kang YH, Jeon SH, Park JY, Chung JH, Choung YH, Choung HW, et al. Platelet-rich fibrin is a bioscaffold and reservoir of growth factors for tissue regeneration. Tissue Eng Part A 2011;17:349-59.
  • [15] Sampson S, Gerhardt M, Mandelbaum B. Platelet rich plasma injection grafts for musculoskeletal injuries: a review. Cur Rev Musculoskelet Med 2008;1:165-74.
  • [16] Zumstein MA, Berger S, Schober M, Boileau P, Nyffeler RW, Horn M, et al. Leukocyte- and platelet-rich fibrin (L-PRF) for long-term delivery of growth factor in rotator cuff repair: review, preliminary results and future directions. Curr Pharm Biotechnol 2012;13:1196-206.
  • [17] Roy S, Driggs J, Elgharably H, Biswas S, Findley M, Khanna S, et al. Platelet-rich fibrin matrix improves wound angiogenesis via inducing endothelial cell proliferation. Wound Repair Regen 2011;19:753-66.
  • [18] Kazemi D, Fakhrjou A, Dizaji VM, Alishahi MK. Effect of autologous platelet rich fibrin on the healing of experimental articular cartilage defects of the knee in an animal model. Biomed Res Int 2014;2014:486436.
  • [19] Hamanishi M1, Nakasa T, Kamei N, Kazusa H, Kamei G, Ochi M. Treatment of cartilage defects by subchondral drilling combined with covering with Atelocollagen membrane induces osteogenesis in a rat model. J Orthop Sci 2013;18:627-35.
  • [20] Vasara AI, Hyttinen MM, Pulliainen O, Lammi MJ, Jurvelin JS, Peterson L, et al. Immature porcine knee cartilage lesions show good healing with or without autologous chondrocyte transplantation. Osteoarthritis Cartilage 2006;14:1066-74.
  • [21] Hurtig MB, Buschmann MD, Fortier LA, Hoemann CD, Hunziker EB, Jurvelin JS, et al. Preclinical studies for cartilage repair: recommendations from the International Cartilage Repair Society. Cartilage 2011;2:137-52.
  • [22] Dahlin RL, Kinard LA, Lam J, Needham CJ, Lu S, Kasper FK, et al. Articular chondrocytes and mesenchymal stem cells seeded on biodegradable scaffolds for the repair of cartilage in a rat osteochondral defect model. Biomaterials 2014;35:7460-9.
  • [23] Jackson DW, Lalor PA, Aberman HM, Simon TM. Spontaneous repair of full thickness defects of articular cartilage in a goat model. A preliminary study. J Bone Joint Surg Am 2001;83:53-64.
  • [24] Kuo TF, Lin MF, Lin YH, Lin YC, Su RJ, Lin HW, et al. Implantation of platelet rich fibrin and cartilage granules facilitates cartilage repair in the injured rabbit knee: preliminary report. Clinics (Sao Paulo) 2011;66:1835-8.

Regenerative effect of platelet-rich fibrin on articular cartilage defects in an experimental rat model

Year 2019, Volume: 5 Issue: 2, 299 - 305, 04.03.2019
https://doi.org/10.18621/eurj.373385

Abstract

Objectives:
Different materials are currently being used experimentally to accelerate
cartilage healing and to obtain hyaline cartilage. We aimed to investigate the
effect of platelet-rich fibrin on joint cartilage healing.

Methods:
Twenty rats were used in this study. platelet-rich fibrin was obtained from
4 rats, and the remaining 16 rats were randomly divided into two groups (4 and
8 weeks). Both knee joints were prepared,
and an osteochondral
defect was created at the femoral condyle
in all rats.
platelet-rich fibrin was placed into the osteochondral defect of the right
knee, while the left knee joint was used as a control. Groups were sacrificed
after 4 and 8 weeks, respectively.

Results:
According to the O'Driscoll score, there were no significant differences
between the right and left knee scores in group 4 weeks
(p = 0.820). However, the right knee scores were
significantly different than the left knee scores in group 8 weeks
(p < 0.001). Defects were completely filled with cartilage tissue
after 8 weeks in the
platelet-rich fibrin group.







Conclusions:
Similar to its role in various tissues, platelet-rich
fibrin
is an effective biomaterial that enhances the healing of osteochondral
defects. 

References

  • [1] Sophia Fox AJ, Bedi A, Rodeo SA. The basic science of articular cartilage: structure, composition, and function. Sports Health 2009;1:461-8.
  • [2] Buma P, Pieper JS, Van Tienen T, Van Susante JL, Van der Kraan PM, Veerkamp JH, et al. Cross-linked type I and type II collagenous matrices for the repair of full-thickness articular cartilage defects: a study in rabbits. Biomaterials 2003;24:3255-63.
  • [3] Dohan Ehrenfest DM, Rasmusson L, Albrektsson T. Classification of platelet concentrates: from pure platelet-rich plasma (P-PRP) to leucocyte- and platelet-rich fibrin (L-PRF). Trends Biotechnol 2009;27:158-67.
  • [4] Choukroun J, Adda F, Schoeffler C, Vervelle A. [An opportunity in perio-implantology: the PRF]. Implantodontie 2001;42:55-62. [Article in French]
  • [5] Choukroun J, Diss A, Simonpieri A, Girard MO, Schoeffler C, Dohan SL, et al. Platelet-rich fibrin (PRF): A second-generation platelet concentrate. Part IV: Clinical effects on tissue healing. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2006;101:e56-60.
  • [6] Choukroun J, Diss A, Simonpieri A, Girard MO, Schoeffler C, Dohan SL, et al. Platelet-rich fibrin (PRF): a second-generation platelet concentrate. Part V: Histologic evaluations of PRF effects on bone allograft maturation in sinus lift. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2006;101:299-303.
  • [7] Dohan DM, Choukroun J, Diss A, Dohan SL, Dohan AJ, Mouhyi J, et al. Platelet-rich fibrin (PRF): a second-generation platelet concentrate. Part II: Platelet-related biologic features. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2006;101:e45-50.
  • [8] Dohan DM, Choukroun J, Diss A, Dohan SL, Dohan AJ, Mouhyi J, et al. Platelet-rich fibrin (PRF): a second-generation platelet concentrate. Part I: Technological concepts and evolution. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2006;101:e37-44.
  • [9] Masuki H, Okudera T, Watanebe T, Suzuki M, Nishiyama K, Okudera H, et al. Growth factor and pro-inflammatory cytokine contents in platelet-rich plasma (PRP), plasma rich in growth factors (PRGF), advanced platelet-rich fibrin (A-PRF), and concentrated growth factors (CGF). Int J Implant Dent 2016;2:19.
  • [10] He L, Lin Y, Hu X, Zhang Y, Wu H. A comparative study of platelet-rich fibrin (PRF) and platelet-rich plasma (PRP) on the effect of proliferation and differentiation of rat osteoblasts in vitro. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2009;108:707-13.
  • [11] O'Driscoll SW, Keeley FW, Salter RB. The chondrogenic potential of free autogenous periosteal grafts for biological resurfacing of major full-thickness defects in joint surfaces under the influence of continuous passive motion. An experimental investigation in the rabbit. J Bone Joint Surg Am 1986;68:1017-35.
  • [12] Stone KR, Walgenbach AW, Freyer A, Turek TJ, Speer DP. Articular cartilage paste grafting to full-thickness articular cartilage knee joint lesions: a 2- to 12- year follow-up. Arthroscopy 2006;22:291-9.
  • [13] Su CY, Kuo YP, Tseng YH, Su CH, Burnouf T. In vitro release of growth factors from platelet-rich fibrin (PRF): a proposal to optimize the clinical applications of PRF. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2009;108:56-61.
  • [14] Kang YH, Jeon SH, Park JY, Chung JH, Choung YH, Choung HW, et al. Platelet-rich fibrin is a bioscaffold and reservoir of growth factors for tissue regeneration. Tissue Eng Part A 2011;17:349-59.
  • [15] Sampson S, Gerhardt M, Mandelbaum B. Platelet rich plasma injection grafts for musculoskeletal injuries: a review. Cur Rev Musculoskelet Med 2008;1:165-74.
  • [16] Zumstein MA, Berger S, Schober M, Boileau P, Nyffeler RW, Horn M, et al. Leukocyte- and platelet-rich fibrin (L-PRF) for long-term delivery of growth factor in rotator cuff repair: review, preliminary results and future directions. Curr Pharm Biotechnol 2012;13:1196-206.
  • [17] Roy S, Driggs J, Elgharably H, Biswas S, Findley M, Khanna S, et al. Platelet-rich fibrin matrix improves wound angiogenesis via inducing endothelial cell proliferation. Wound Repair Regen 2011;19:753-66.
  • [18] Kazemi D, Fakhrjou A, Dizaji VM, Alishahi MK. Effect of autologous platelet rich fibrin on the healing of experimental articular cartilage defects of the knee in an animal model. Biomed Res Int 2014;2014:486436.
  • [19] Hamanishi M1, Nakasa T, Kamei N, Kazusa H, Kamei G, Ochi M. Treatment of cartilage defects by subchondral drilling combined with covering with Atelocollagen membrane induces osteogenesis in a rat model. J Orthop Sci 2013;18:627-35.
  • [20] Vasara AI, Hyttinen MM, Pulliainen O, Lammi MJ, Jurvelin JS, Peterson L, et al. Immature porcine knee cartilage lesions show good healing with or without autologous chondrocyte transplantation. Osteoarthritis Cartilage 2006;14:1066-74.
  • [21] Hurtig MB, Buschmann MD, Fortier LA, Hoemann CD, Hunziker EB, Jurvelin JS, et al. Preclinical studies for cartilage repair: recommendations from the International Cartilage Repair Society. Cartilage 2011;2:137-52.
  • [22] Dahlin RL, Kinard LA, Lam J, Needham CJ, Lu S, Kasper FK, et al. Articular chondrocytes and mesenchymal stem cells seeded on biodegradable scaffolds for the repair of cartilage in a rat osteochondral defect model. Biomaterials 2014;35:7460-9.
  • [23] Jackson DW, Lalor PA, Aberman HM, Simon TM. Spontaneous repair of full thickness defects of articular cartilage in a goat model. A preliminary study. J Bone Joint Surg Am 2001;83:53-64.
  • [24] Kuo TF, Lin MF, Lin YH, Lin YC, Su RJ, Lin HW, et al. Implantation of platelet rich fibrin and cartilage granules facilitates cartilage repair in the injured rabbit knee: preliminary report. Clinics (Sao Paulo) 2011;66:1835-8.
There are 24 citations in total.

Details

Primary Language English
Subjects Health Care Administration
Journal Section Original Articles
Authors

Hasan Metineren 0000-0002-5671-6273

Turan Cihan Dülgeroğlu 0000-0002-9661-5418

Publication Date March 4, 2019
Submission Date January 1, 2018
Acceptance Date July 1, 2018
Published in Issue Year 2019 Volume: 5 Issue: 2

Cite

AMA Metineren H, Dülgeroğlu TC. Regenerative effect of platelet-rich fibrin on articular cartilage defects in an experimental rat model. Eur Res J. March 2019;5(2):299-305. doi:10.18621/eurj.373385

e-ISSN: 2149-3189 


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