Cu/Zn superoxide dismutase enzyme immunoreactivity in the stomach tissue of rats fed with mussels (Mytilus galloprovincialis)

Year 2019, Volume: 5 Issue: 2, 319 - 325, 04.03.2019

Aslı Muratlı Mehmet Rıza Gezen

https://doi.org/10.18621/eurj.411686

Abstract

Objectives: Mussels accumulate heavy metals in their tissues.
Although there are few data about the toxicity of seafood that is exposed to
environmental pollution, there are no animal studies about the gastric toxicity
of mussels grown in the Dardanelles. The antioxidant Cu/Zn superoxide dismutase
(Cu/Zn SOD) enzyme catalyzes the hydrogen peroxide dismutation of superoxide
radicals and removes the effects of free radicals which cause oxidative stress.
The purpose of the study was to demonstrate the Cu/Zn SOD in the stomach
tissues of rats which are fed with mussels that are collected from the Çamburnu
region of the Dardanelles. 

Methods: A total of 24 male Wistar albino rats were randomly divided into four
groups: Group 1 (n = 6), control group fed with standard rat food; Group 2 (n =
6), 75% mussels and 25% standard rat food daily; Group 3 (n = 6), 75% mussels
and 25% standard rat food every two days; and Group 4 (n = 6), 75% mussels and
25% standard rat food every three days. To detect Cu/Zn SOD localization in the
tissues, the LAB-SA Detection System was used. 

Results: Cu/Zn SOD enzyme immunoreactivity was not detected in Group 1 and in
samples without Cu/Zn SOD primer antibody. Cu/Zn SOD enzyme immunoreactivity
was detected 82% in Group 2, 79% in Group 3, and 61% in Group 4. There was
statistically significant difference between the Cu/Zn SOD immunoreactivity of
epithelial cells in the gastric mucosa of the rats in the experimental and
control groups (p < 0.05). 

Conclusions: Determination of the increase of Cu/Zn SOD enzyme in
the gastric mucosa of mussel-fed rats that we used in our study suggests that
it triggers the antioxidant defense mechanism against mussel toxicity.

Keywords

Immunohistochemistry, Dardanelles, mussel, Cu/Zn superoxide dismutase, stomach

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