Evaluation of high molecular weight cytokeratin (HMWCK), p27, C-X-C chemokine receptor type 4 (CXCR-4) and stromal cell-derived factor 1 (SDF-1) expressions related to tumor progression in breast cancer

Year 2019, Volume: 5 Issue: 5, 800 - 808, 04.09.2019

Gonca Özgün , Gülen Akyol

https://doi.org/10.18621/eurj.423593

Abstract

Objectives:
Breast cancer is the
most common cancer and the most common reason for cancer death in women
population. The immunohistochemical markers which could have prognostic
information are always needed.

Methods: This study included 365 cases of invasive ductal
carcinoma (IDC), ductal carcinoma in situ (DCIS) and ductal epithelial
hyperplasia. The cases divided into the following two groups according to the
presence of cancer: 1) cancer group (298 cases; cases with IDC and DCIS), 2)
non-cancer group (67 cases without cancer; cases with usual ductal epithelial
hyperplasia [UDH] and atypical ductal epithelial hyperplasia [ADH]). All histological slides
stained with high molecular weight
cytokeratin (HMWCK), p27, C-X-C
chemokine receptor type 4 (CXCR-4), stromal cell-derived factor 1 (SDF-1) immunohistochemically.

Results: IDC was present in 277 cases, of which 213 had pure
IDC, and 64 had DCIS component adjacent to the invasive tumor. Twenty-one cases
had only DCIS. Of 67 cases with epithelial hyperplasia, 31 had ADH, and 36 had UDH. Among cases with IDC, 143 had lymph node excision,
of which 73 had metastasis in one or more lymph nodes, and 70 did not have
metastatic disease. The expression of p27 was found to be significantly lower
in the cancer group as compared to that in the non-cancer group (p < 0.0001).
CXCR-4 expression in IDC was found to be higher than that of DCIS group. SDF-1
expression was observed to be significantly higher in cancer cases than that of
non-cancer cases (p = 0.03).

Conclusions: The higher CXCR-4 and SDF-1 expressions are associated
with tumor progression, tumor size, and lymph node status. In benign
proliferative lesions, both HMWK and p27 expressions were helpful in
differential diagnosis of borderline atypical ductal hyperplasia and DCIS. 

Keywords

CXCR-4, SDF-1, p27, HMWCK, benign proliferative breast lesions, ductal carcinoma in situ, invasive ductal carcinoma, tissue microarray

Supporting Institution

Gazi University Scientific Research Projects Unit

Project Number

SBE-01/2007-104

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