Early bacterial identification and carbapenemase detection from positive blood culture by mass spectrometry and Blue-Carba test

Year 2020, Volume: 6 Issue: 2, 154 - 162, 04.03.2020

María Eugenia Cattani Sandra Cogut Sara C. Kaufman

https://doi.org/10.18621/eurj.469655

Abstract

Objectives:
In this study, we evaluated a rapid and simple protocol for direct
identification of microorganisms with Matrix-Assisted Laser
Desorption/Ionization Time of Flight, Mass Spectrometry (MALDI-TOF MS) after a
short incubation in solid medium (3 to 5h).

Methods: We have examined a total
of 1101 positive blood culture bottles from 782 patients.

Results: We obtained a correct
identification in 1037 (94.18%); 190 (98.44%) were Staphylococcus aureus, and 386 (98.44%) Enterobacteriaceae. Both are the most
frequent etiological agents of sepsis. A total of 1004 bottles were monomicrobial
(96.81%) and 33 (3.18%), polimicrobial. In the latter we identified at least one
species. Two hundred eighty (27.00%) organism isolated were considered skin
contaminant. Carbapenemase tests were performed with Blue-Carba test in 140
patinas from Gram negative bacilli, we have detected earlier 27 of 29 positive
(93.10%).

Conclusions: Mass spectrometry by MALDI-TOF MS is very useful
to quickly identify the microbial agent and Blue-Carba contribute to adapt the
antibiotic therapy to obtain a correct clinical management of the patient with
bacteremia.

Keywords

MALDI-TOF MS, rapid identification, blood culture, carbapenemase, short incubation

Thanks

We are grateful to Tamara Posse and Luis Aquino for their technical assistance.

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