Year 2020, Volume 6 , Issue 2, Pages 154 - 162 2020-03-04

Early bacterial identification and carbapenemase detection from positive blood culture by mass spectrometry and Blue-Carba test

María Eugenia CATTANİ [1] , Sandra COGUT [2] , Sara C. KAUFMAN [3]


Objectives: In this study, we evaluated a rapid and simple protocol for direct identification of microorganisms with Matrix-Assisted Laser Desorption/Ionization Time of Flight, Mass Spectrometry (MALDI-TOF MS) after a short incubation in solid medium (3 to 5h).

Methods: We have examined a total of 1101 positive blood culture bottles from 782 patients.

Results: We obtained a correct identification in 1037 (94.18%); 190 (98.44%) were Staphylococcus aureus, and 386 (98.44%) Enterobacteriaceae. Both are the most frequent etiological agents of sepsis. A total of 1004 bottles were monomicrobial (96.81%) and 33 (3.18%), polimicrobial. In the latter we identified at least one species. Two hundred eighty (27.00%) organism isolated were considered skin contaminant. Carbapenemase tests were performed with Blue-Carba test in 140 patinas from Gram negative bacilli, we have detected earlier 27 of 29 positive (93.10%).

Conclusions: Mass spectrometry by MALDI-TOF MS is very useful to quickly identify the microbial agent and Blue-Carba contribute to adapt the antibiotic therapy to obtain a correct clinical management of the patient with bacteremia.

MALDI-TOF MS, rapid identification, blood culture, carbapenemase, short incubation
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Primary Language en
Subjects Microbiology
Journal Section Original Articles
Authors

Orcid: 0000-0002-5749-6540
Author: María Eugenia CATTANİ
Institution: Sección Microbiología Clínica, División Laboratorio, Hospital Juan A. Fernández, Ciudad Autónoma de Buenos Aires, Argentina
Country: Argentina


Orcid: 0000-0002-7039-352X
Author: Sandra COGUT
Institution: Sección Microbiología Clínica, División Laboratorio, Hospital Juan A. Fernández, Ciudad Autónoma de Buenos Aires, Argentina
Country: Argentina


Orcid: 0000-0002-1357-6776
Author: Sara C. KAUFMAN (Primary Author)
Institution: Sección Microbiología Clínica, División Laboratorio, Hospital Juan A. Fernández, Ciudad Autónoma de Buenos Aires, Argentina
Country: Argentina


Thanks We are grateful to Tamara Posse and Luis Aquino for their technical assistance.
Dates

Publication Date : March 4, 2020

EndNote %0 The European Research Journal Early bacterial identification and carbapenemase detection from positive blood culture by mass spectrometry and Blue-Carba test %A María Eugenia CATTANİ , Sandra COGUT , Sara C. KAUFMAN %T Early bacterial identification and carbapenemase detection from positive blood culture by mass spectrometry and Blue-Carba test %D 2020 %J The European Research Journal %P -2149-3189 %V 6 %N 2 %R doi: 10.18621/eurj.469655 %U 10.18621/eurj.469655