Year 2020, Volume 6 , Issue 6, Pages 534 - 538 2020-11-04

Dehydrated human amnion/chorion membrane allograft for preventing epidural fibrosis after laminectomy: an experimental rat model

Özhan Merzuk UÇKUN [1] , Denizhan DİVANLIOĞLU [2] , Rıfat AKDAĞ [3] , Güner MENEKŞE [4] , Ali DALGIÇ [5] , Ahmet Deniz BELEN [6]


Objectives: Post-laminectomy syndrome due to epidural fibrosis (EF) is a common cause of persistent low back pain after lumbar spinal surgery and is challenging for both surgeons and patients. The current experimental study aimed to investigate the effect of dehydrated human amnion/chorion membrane (dHACM) allograft for preventing EF formation following lumbar laminectomy.

Methods: Sixteen Sprague–Dawley rats equally divided into two groups underwent lumbar laminectomy. Group A, the control group, underwent lumbar laminectomy with standard closure. Group B, the experimental group, additionally received epidural dHACM allografts during the procedure. After 45 days of follow-up, the rats were sacrificed, and the harvested tissues were histopathologically evaluated for EF.

Results: Compared with Group A, Group B showed significantly less EF generation (p < 0.001), implying that dHACM allografts effectively prevent EF.

Conclusions: This study demonstrated that dHACM can effectively reduce EF formation after spinal laminectomy in rats.

Epidural fibrosis, laminectomy, amnion membrane allograft
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Primary Language en
Subjects Neurosciences
Journal Section Original Articles
Authors

Orcid: 0000-0002-3845-2665
Author: Özhan Merzuk UÇKUN (Primary Author)
Institution: Department of Neurosurgery, University of Health Sciences, Ankara Numune Training and Research Hospital, Ankara, Turkey
Country: Turkey


Orcid: 0000-0003-0267-196X
Author: Denizhan DİVANLIOĞLU
Institution: Department of Neurosurgery, University of Health Sciences, Ankara Numune Training and Research Hospital, Ankara, Turkey
Country: Turkey


Orcid: 0000-0001-7638-8361
Author: Rıfat AKDAĞ
Institution: Department of Neurosurgery, University of Health Sciences, Bursa Yüksek İhtisas Training and Research Hospital, Bursa, Turkey
Country: Turkey


Orcid: 0000-0002-5912-6949
Author: Güner MENEKŞE
Institution: Department of Neurosurgery, University of Health Sciences, Ankara Training and Research Hospital, Ankara, Turkey
Country: Turkey


Orcid: 0000-0003-1000-2811
Author: Ali DALGIÇ
Institution: Department of Neurosurgery, University of Health Sciences, Ankara Numune Training and Research Hospital, Ankara, Turkey
Country: Turkey


Orcid: 0000-0001-8482-4725
Author: Ahmet Deniz BELEN
Institution: Department of Neurosurgery, University of Health Sciences, Ankara Numune Training and Research Hospital, Ankara, Turkey
Country: Turkey


Dates

Application Date : February 7, 2019
Acceptance Date : March 20, 2019
Publication Date : November 4, 2020

EndNote %0 The European Research Journal Dehydrated human amnion/chorion membrane allograft for preventing epidural fibrosis after laminectomy: an experimental rat model %A Özhan Merzuk Uçkun , Denizhan Di̇vanlıoğlu , Rıfat Akdağ , Güner Menekşe , Ali Dalgıç , Ahmet Deniz Belen %T Dehydrated human amnion/chorion membrane allograft for preventing epidural fibrosis after laminectomy: an experimental rat model %D 2020 %J The European Research Journal %P -2149-3189 %V 6 %N 6 %R doi: 10.18621/eurj.523854 %U 10.18621/eurj.523854