Year 2020, Volume 6 , Issue 6, Pages 539 - 544 2020-11-04

Evaluation of the effects on atherosclerosis and antioxidant and antimicrobial activities of Agaricus xanthodermus poisonous mushroom

Betül ÖZALTUN [1] , Mustafa SEVİNDİK [2]


Objectives: The aim of this study was to determine the total antioxidant capacity, total oxidant capacity, oxidative stress index and antimicrobial activity of a poisonous mushroom Agaricus xanthodermus. The effects of mushrooms on atherosclerosis are due to their antioxidant effects.

Methods: Mushroom samples collected from study field were extracted with methanol (MeOH) and dichloromethane (DCM) using soxhlet apparatus. Total antioxidant status (TAS), total oxidant status (TOS) and oxidative stress index (OSI) were measured using Rel Assay trade kits. Antimicrobial activities were tested on 9 microorganisms (Staphylococcus aureus, S. aureus MRSA, Enterococcus faecalis, Escherichia coli, Pseudomonas aeruginosa, Acinetobacter baumannii, Candida albicans, C.krusei and C. glabrata) using the modified agar dilution method.

Results: In this study A. xanthodermus has shown high antioxidant and antimicrobial activities. In addition, the highest activities of MeOH and DCM extracts of the mushrooms were demonstrated against E. coli, P. aeruginosa, and A. baumannii.

Conclusions: In conclusion, A. xanthodermus is considered to be a poisonous mushroom and can be used as a pharmacological natural agent due to its high antioxidant and antimicrobial activities.

Medicinal mushroom, poisonous mushrooms, Agaricus xanthodermus, antioxidant, antimicrobial, atherosclerosis
  • 1. Lima AD, Costa Fortes R, Garbi Novaes MRC, Percário S. Poisonous mushrooms; a review of the most common intoxications. Nutr Hosp 2012;27:402-8.
  • 2. Jo WS, Hossain MA, Park SC. Toxicological profiles of poisonous, edible, and medicinal mushrooms. Mycobiology 2014;42:215-20.
  • 3. White J, Warrel D, Eddleston M, Currie BJ, White IM, Isbister GK. Clinical toxinology --Where are we now? J Toxicol Clin Toxicol 2003;41:263-76.
  • 4. Flesch F, Saviuc P. [Mushroom poisoning: syndromes and treatment] EMC-Médecine 2004;1:70-9. [Article in French]
  • 5. Ecevit Ç, Hızarcıoğlu M, Gerçek PA, Gerçek H, Kayserili E, Gülez P, et al. [Evaluation of musroom intoxications presenting at the emercency department of Dr. Behçet Uz children's hospital]. ADÜ Tıp Fak Derg 2004;5(3):11-14. [Article in Turkish]
  • 6. Catcheside P, Yellow Stainer, State Herbarium Factsheet, Government of South Australia, 2017.
  • 7. Hender E, May T, Beulke S. Poisoning due to eating fungi in Victoria. Aust Fam Physician 2000;29:1000-4.
  • 8. Beug W, Shaw M, Cochran KW. Thirty plus years of mushroom poisoning: summary of the approximately 2,000 reports in the NAMA case registry. McIlvainea 2006;16:47-68.
  • 9. Valverde ME, Hernández-Pérez T, Paredes-López O. Edible mushrooms: improving human health and promoting quality life. Int J Microbiol 2015; 2015:376387.
  • 10. Calvo AM, Wilson RA, Bok JW, Keller NP. Relationship between secondary metabolism and fungal development. Microbiol Mol Biol Rev 2002;66:447-59.
  • 11. Wieczorek Z, Siemion IZ, Bolewska-Pedyczak E, Wieland T. Immunosuppressive activity in the series of cycloamanide peptides from mushrooms. Peptides 1993;14:1-5.
  • 12. Badalyan SM, Innocenti G, Garibyan NG. Antagonistic activity of xylotrophic mushrooms against pathogenic fungi of cereals in dual culture. Phytopathol Mediterr 2002;41;220-5.
  • 13. Lull C, Wichers HJ, Savelkoul HF. Antiinflammatory and immunomodulating properties of fungal metabolites. Mediators Inflamm 2005;2:63-80.
  • 14. Ede SO, Olaniru E, Otimenyin S, Aguiyi JC, Ekwere EO. Analgesic and anti inflammatory activities of the ethanolic extract of the mushroom Ganoderma applanatum. IJRRAS 2012;13:349-52.
  • 15. Moharib SA, El Maksoud NA, Ragab HM. Anticancer activities of mushroom polysaccharides on chemically-induced colorectal cancer in rats. J Appl Pharma Sci 2014;4:54-63.
  • 16. Merdivan S, Lindequist U. Medicinal mushrooms with antiallergic activities. In: Agrawal DC, Tsay HS, Shyur LF, Wu YC, Wang SY, eds. Medicinal Plants and Fungi: Recent Advances in Research and Development. Springer: Singapore. 2017: pp. 93-110.
  • 17. Yılmaz A, Yıldız S, Kılıç C, Can Z. Total phenolics, flavonoids, tannin contents and antioxidant properties of Pleurotus ostreatus cultivated on different wastes and sawdust. Int J Sec Metabolite 2017;4:1-9.
  • 18. WHO. WHO technical report series. Diet, Nutrition and the Prevention of Chronic Disease. 2003.
  • 19. Assmann G, Cullen P, Schulte H. Simple scoring scheme for calculating the risk of acute coronary events based on the 10-year follow-up of the prospective cardiovascular Munster (PROCAM) study. Circulation 2002;105:310-15.
  • 20. Cheung LM, Cheung PC, Ooi VE. Antioxidant activity and total phenolics of edible mushroom extracts. Food Chem 2003;81:249-55.
  • 21. Wong JY, Chye FY. Antioxidant properties of selected tropical wild edible mushrooms. J Food Compost Anal 2009:22:269-77.
  • 22. Jose N, Ajith TA, Janardhanan KK. Methanol extract of the oyster mushroom, Pleurotus florida, inhibits inflammation and platelet aggregation. Phytother Res 2004;18:43-6.
  • 23. Kohno K, Miyake M, Sano O, Tanaka-Kataoka M, Yamamoto S, Koya-Miyata S, et al. Anti-inflammatory and immunomodulatory properties of 2-amino-3H-phenoxazin-3-one. Biol Pharma Bull 2008;31:1938-45.
  • 24. Visioli F, Poli A, Richard D, Paoletti R. Modulation of inflammation by nutritional interventions. Curr Atheroscler Rep 2008;10:451-3.
  • 25. García-Lafuente A, Guillamón E, Villares A, Rostagno MA, Martínez JA. Flavonoids as anti-inflammatory agents: implications in cancer and cardiovascular disease. Inflamm Res 2009;58:537-52.
  • 26. Balsano C, Alisi A. Antioxidant effects of natural bioactive compounds. Curr Pharma Des 2009;15:3063-73.
  • 27. Witztum JL, Steinberg D. The oxidative modification hypothesis of atherosclerosis: does it hold for humans? Trends Cardiovasc Med 2001;11:93-102.
  • 28. Erel O. A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radical cation. Clin Biochem 2004;37:277-85.
  • 29. Erel O. A new automated colorimetric method for measuring total oxidant status. Clin Biochem 2005;38:1103-11.
  • 30. Bauer AW, Kirby WM, Sherris, JC, Turck M. Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol 1966;45:493-6.
  • 31. Hindler J, Hochstein L, Howell A. Preparation of routine media and reagents used in antimicrobial susceptibility testing. Part 1. McFarland standards, p. 5.19.1-5.19.6. In: Isenberg HD, ed., Clinical microbiology procedures handbook, vol. 1. American Society for Microbiology, Washington, D.C. 1992.
  • 32. EUCAST (European Committee on Antimicrobial Susceptibility Testing). Breakpoint tables Fungal isolate for interpretation of MICs. Version 7.0, 2014.
  • 33. Matuschek E, Brown DF, Kahlmeter G. Development of the EUCAST disk diffusion antimicrobial susceptibility testing method and its implementation in routine microbiology laboratories. Clin Microbiol Infect 2014;20:255-66.
  • 34. EUCAST (European Committee on Antimicrobial Susceptibility Testing). Breakpoint tables for Bacteria interpretation of MICs and zone diameters, Version 5.0, 2015.
  • 35. Bala N, Aitken EA, Fechner N, Cusack A, Steadman KJ. Evaluation of antibacterial activity of Australian basidiomycetous macrofungi using a high-throughput 96-well plate assay. Pharma Biol 2011;49:492-500.
  • 36. Akgul H, Sevindik M, Coban C, Alli H, Selamoglu Z. New approaches in traditional and complementary alternative medicine practices: Auricularia auricula and Trametes versicolor. J Tradit Med Clin Natur 2017;6:239.
  • 37. Sevindik M, Akgul H, Bal C, Selamoglu Z. Phenolic contents, oxidant/antioxidant potential and heavy metal levels in Cyclocybe cylindracea. Indian J Pharma Edu Res 2018;52:437-41.
  • 38. Bal C. A study on antioxidant properties of Gyrodon lividus. Eurasia J Forest Sci 2018;6:40-3.
  • 39. Sevindik M. Investigation of oxidant and antioxidant status of edible mushroom Clavariadelphus truncatus. Mantar Dergisi 2018;9:165-8.
  • 40. Sevindik M, Akgül H, Bal C. Determination of oxidative stress status of Ompholatus olearius gathered from Adana and Antalya Provinces in Turkey. Sakarya University Journal of Science 2017;21:324-7.
  • 41. Li H, Horke S, Förstermann U. Oxidative stress in vascular disease and its pharmacological prevention. Trends Pharmacol Sci 2013;34:313-9.
  • 42. Su PW, Yang CH, Yang JF, Su PY, Chuang LY. Antibacterial activities and antibacterial mechanism of Polygonum cuspidatum extracts against nosocomial drug-resistant pathogens. Molecules 2015;20:11119-30.
  • 43. Gebreyohannes G, Nyerere A, Bii C, Berhe Sbhatu D. Determination of antimicrobial activity of extracts of indigenous wild mushrooms against pathogenic organisms. Evid Based Complement Alternat Med 2019;2019:6212673.
  • 44. Mariselvi M, Ninganna E. Molecular identification and screening of mushrooms for antibacterial property against Pseudomonas aeruginosa and Staphylococcus aureus. J App Nat Sci 2018;10:791-6.
Primary Language en
Subjects Cardiac and Cardiovascular Systems, Health Care Sciences and Services
Journal Section Original Articles
Authors

Orcid: 0000-0003-4725-9522
Author: Betül ÖZALTUN
Institution: Department of Cardiology, Niğde Ömer Halisdemir University of School of Medicine, Niğde, Turkey
Country: Turkey


Orcid: 0000-0001-7223-2220
Author: Mustafa SEVİNDİK (Primary Author)
Institution: Department of Food Processing, Osmaniye Korkut Ata University, Bahçe Vocational High School, Osmaniye, Turkey
Country: Turkey


Dates

Application Date : February 8, 2019
Acceptance Date : April 24, 2019
Publication Date : November 4, 2020

EndNote %0 The European Research Journal Evaluation of the effects on atherosclerosis and antioxidant and antimicrobial activities of Agaricus xanthodermus poisonous mushroom %A Betül Özaltun , Mustafa Sevi̇ndi̇k %T Evaluation of the effects on atherosclerosis and antioxidant and antimicrobial activities of Agaricus xanthodermus poisonous mushroom %D 2020 %J The European Research Journal %P -2149-3189 %V 6 %N 6 %R doi: 10.18621/eurj.524149 %U 10.18621/eurj.524149