Year 2021, Volume 7 , Issue 2, Pages 152 - 158 2021-03-04

Optimization of culture conditions for antibacterial substance production from newly isolated Brevibacillus laterosporus EA62

Elif DEMİRKAN [1] , Aynur AYBEY [2] , Alev USTA [3]

Objectives: In the present study, it was reported the effects of some nutritional (amino acid, carbon, nitrogen and metal sources) and physical factors (pH and temperature) on antibacterial substance activity of Brevibacillus laterosporus EA62.

Methods: The agar well diffusion assay was performed to evaluate the antibacterial activity of the substance. The antibacterial activity of the new substance was examined against four pathogenic bacteria under different nutritional and physical conditions.

Results: The best antibacterial activity was obtained in modified medium consists of the 5% glucose, 0.1% tryptone, 0.05% MgSO4+CaCO3 and 0.5% glutamic acid. For physical parameters, maximal activity was observed after 72 h when incubated at 37°C, pH 7.0.

Conclusions: This study indicates that Brevibacillus laterosporus EA62 could be an important source of antibacterial substances under this medium optimization.

Antibacterial substance, Brevibacillus, physical, nutritional
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Primary Language en
Subjects Microbiology
Journal Section Original Articles

Orcid: 0000-0002-5292-9482
Author: Elif DEMİRKAN (Primary Author)
Institution: Department of Biology, Bursa Uludağ University, Faculty of Arts and Sciences, Bursa, Turkey
Country: Turkey

Orcid: 0000-0003-2743-9745
Author: Aynur AYBEY
Institution: Department of Biology, Bursa Uludağ University, Faculty of Arts and Sciences, Bursa, Turkey
Country: Turkey

Orcid: 0000-0002-9728-0066
Author: Alev USTA
Institution: Department of Biology, Bursa Uludağ University, Faculty of Arts and Sciences, Bursa, Turkey
Country: Andorra


Application Date : August 7, 2019
Acceptance Date : September 16, 2020
Publication Date : March 4, 2021

EndNote %0 The European Research Journal Optimization of culture conditions for antibacterial substance production from newly isolated Brevibacillus laterosporus EA62 %A Elif Demirkan , Aynur Aybey , Alev Usta %T Optimization of culture conditions for antibacterial substance production from newly isolated Brevibacillus laterosporus EA62 %D 2021 %J The European Research Journal %P -2149-3189 %V 7 %N 2 %R doi: 10.18621/eurj.603491 %U 10.18621/eurj.603491