Year 2021, Volume 7 , Issue 3, Pages 218 - 224 2021-05-04

Effect of the Wnt/β-catenin pathway inhibitors on cell proliferation and migration of HEC-1A endometrial adenocarcinoma: experimental cell culture model

Yaşam ÇİRÇİRCİ [1] , R. Nalan TİFTİK [2] , İsmail ÜN [3]


Objectives: The most diagnosed tumor among infiltrating tumors of the female genital tract is endometrial carcinoma. The Wnt/β-catenin signaling pathway has an important role in organogenesis, self-renewal of tissues, and adult stem cell maintenance. However aberrant activation of it causes many types of tumors and also related to the prognosis of patients. Therefore, we aimed to investigate whether Wnt/β-catenin pathway inhibitors have any effect on the proliferation and migration of tumor cells.

Methods: As cancer cell line, HEC-1A endometrial adenocarcinoma was used. The Wnt/β-catenin pathway inhibitors effects on proliferation and migration were demonstrated by real-time cell analysis device and wound healing model respectively.

Results: Wnt/β-catenin pathway inhibitors FH535 (25 μM at 36th hour, p < 0.05; 50μM at 48th hour p < 0.001) and niclosamide inhibited cell proliferation (10, 25 and 50μM at 60th hours; p < 0.01, p < 0.05 and p < 0.001, respectively) whereas ICRT14 and IWP-2 did not. However only niclosamide which is also an antihelmintic drug inhibited migration of the cells in all concentrations tested (10, 25 and 50μM, p < 0.05).

Conclusions: The present study shows that the Wnt/β-catenin pathway has an substantial role in both proliferation and migration of endometrial adenocarcinoma. We suggest that the antihelmintic drug niclosamide could be further investigated for its potential therapeutic effect in endometrial adenocarcinoma.

Wnt/β-catenin, Endometrial adenocarcinoma, Niclosamide, Migration
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Primary Language en
Subjects Medicine
Journal Section Original Articles
Authors

Orcid: 0000-0003-0283-6955
Author: Yaşam ÇİRÇİRCİ
Institution: Department of Medical Pharmacology, Mersin University School of Medicine, Mersin, Turkey
Country: Turkey


Orcid: 0000-0001-7277-3369
Author: R. Nalan TİFTİK (Primary Author)
Institution: Department of Medical Pharmacology, Mersin University School of Medicine, Mersin, Turkey
Country: Turkey


Orcid: 0000-0001-6442-4185
Author: İsmail ÜN
Institution: Department of Medical Pharmacology, Mersin University School of Medicine, Mersin, Turkey
Country: Turkey


Supporting Institution Mersin University Scientific Research Projects Center
Project Number 2018-2-TP2-3022
Dates

Application Date : March 22, 2021
Acceptance Date : March 29, 2021
Publication Date : May 4, 2021

EndNote %0 The European Research Journal Effect of the Wnt/β-catenin pathway inhibitors on cell proliferation and migration of HEC-1A endometrial adenocarcinoma: experimental cell culture model %A Yaşam Çirçirci , R. Nalan Tiftik , İsmail Ün %T Effect of the Wnt/β-catenin pathway inhibitors on cell proliferation and migration of HEC-1A endometrial adenocarcinoma: experimental cell culture model %D 2021 %J The European Research Journal %P -2149-3189 %V 7 %N 3 %R doi: 10.18621/eurj.900847 %U 10.18621/eurj.900847