Year 2021, Volume 7 , Issue 4, Pages 348 - 355 2021-07-04

Comparison of the effect of ethanol and potassium iodide in antibacterial photodynamic therapy on gram negative pathogens

Nermin TOPALOĞLU AVŞAR [1] , Melike ÇAĞAN [2] , Emel BAKAY [3] , Aziz KOLKIRAN [4]


Objectives: Antibiotics is the most common treatment for bacterial infections. However, bacteria can change their genetic material, develop antibiotic resistance and cannot be treated. This brings the need for new treatment methods. Antibacterial Photodynamic Therapy is becoming a promising approach to treat bacterial infections. It is based on the use of photosensitizer to be activated by light with an appropriate wavelength and it will result in reactive oxygen species which are responsible for the destruction of pathogens. In this study, Antibacterial Photodynamic Therapy was examined for the treatment of Pseudomonas aeruginosa with the help of ethanol and potassium iodide.

Methods: The effect of Chlorin e6-based photoinactivation was studied on Multidrug resistant P. aeruginosa upon irradiation with 655-nm diode laser. Then ethanol and potassium iodide was added to the mechanism separately to increase the efficacy of photoinactivation. After each application, serial dilution method was used for the determination of viable cells.

Results: Outcomes showed that only Antibacterial Photodynamic Therapy causes a mortality rate of 75%. Addition of ethanol causes a mortality rate of 93% and addition of potassium iodide causes a mortality rate of 99.9% with less amount of Chlorin e6 and light dose.

Conclusions: Chlorin-e6 based photoinactivation did not provide high mortality rate on P. aeruginosa. The use of ethanol and potassium iodide increased the effect of photoinactivation. The highest mortality rate was obtained with potassium iodide. It was understood that potassium iodide was a better concomitant agent to increase the bactericidal effect of Antibacterial Photodynamic Therapy on P. aeruginosa.

Antibacterial photodynamic therapy, chlorin e6, ethanol, potassium iodide, pseudomonas aeruginosa
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Primary Language en
Subjects Infectious Diseases, Microbiology
Journal Section Original Articles
Authors

Orcid: 0000-0001-7001-8327
Author: Nermin TOPALOĞLU AVŞAR (Primary Author)
Institution: Department of Biomedical Engineering, Izmir Katip Celebi University, Faculty of Engineering and Architecture, Izmir, Turkey
Country: Turkey


Orcid: 0000-0002-0616-6488
Author: Melike ÇAĞAN
Institution: Department of Bioengineering, Izmir Institute of Technology, Izmir, Turkey
Country: Turkey


Orcid: 0000-0002-3042-810X
Author: Emel BAKAY
Institution: Department of Biomedical Engineering, Izmir Katip Celebi University, Faculty of Engineering and Architecture, Izmir, Turkey
Country: Turkey


Orcid: 0000-0002-7440-5428
Author: Aziz KOLKIRAN
Institution: Department of Engineering Sciences, Izmir Katip Celebi University, Faculty of Engineering and Architecture, Izmir, Turkey
Country: Turkey


Supporting Institution İzmir Katip Çelebi University Scientific Research Projects
Project Number 2015-ÖNP-MÜMF-0017
Thanks The authors thank Nesrin Horzum Polat, Fatma İbiş and Nur Çobanoğlu for their help and valuable opinions.
Dates

Application Date : May 15, 2020
Acceptance Date : March 24, 2021
Publication Date : July 4, 2021

EndNote %0 The European Research Journal Comparison of the effect of ethanol and potassium iodide in antibacterial photodynamic therapy on gram negative pathogens %A Nermin Topaloğlu Avşar , Melike Çağan , Emel Bakay , Aziz Kolkıran %T Comparison of the effect of ethanol and potassium iodide in antibacterial photodynamic therapy on gram negative pathogens %D 2021 %J The European Research Journal %P -2149-3189 %V 7 %N 4 %R doi: 10.18621/eurj.736340 %U 10.18621/eurj.736340