Dye Ligand Affinity Nanoparticles for the Depletion of Biomolecules in Proteomics

Abstract

Objective: Serum proteins are indicators for certain diseases. However, detection of the biomarkers is difficult because the more abundant proteins mask the less abundant ones. The depletion of abundant serum proteins will help in the discovery and detection of less abundant proteins that may prove to be informative disease markers. Dye ligands have attracted great attention because of their cost-effectiveness, easy immobilization, stability, and high binding capacity. Due to these advantageous properties, dye ligands have also been chosen as an alternative for biological ligands such as antibodies, enzymes, etc.

Materials and Methods: Poly (ethylene glycol dimethacrylate) [p (EGDM)] nanoparticles were prepared using the surfactant-free emulsion polymerization technique. Then, Reactive Red 120 (RR 120) dye was immobilized to nanoparticles in a nucleophilic substitution reaction. The RR120 attached nanoparticles were characterized.

Results: The size/size distribution of p (EGDM) nanoparticles was measured with a Zeta sizer. The scanning electron microscope (SEM) images were found to support a measurement of around 100 nm. The maximum adsorbed amount of albumin was observed at pH 6.0. The maximum depleted albumin concentration was found to be 453.9 mg/g nanoparticles according to the experimental results. Desorption studies were carried out by addition of 0.5 M of KSCN to the albumin solutions. The desorption results showed that the binding of albumin to the nanoparticle was reversible.

Conclusion: Our results demonstrated that dye attached nanoparticles have the potential for depleting albumin from serum in proteomics

Keywords

• Proteomics
• dye ligand
• nanoparticles
• Reactive Red 120
• biomolecules
• albumin

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