Lomber Disk Hernisi Tanısı Konan Türk Hastalarda Vitamin D Reseptör (VDR) Gen Polimorfizmlerinin Araştırılması

Abstract

Amaç: Lomber disk hernisi (LDH) yaygın bir dejeneratif hastalıktır. D vitamini yolu ile hastalığın etyopatogenezi arasında olası bir ilişkinin varlığı çalışmalarda tam olarak gösterilememiştir. Bu çalışmada LDH hastalarında D vitamini düzeyini etkilediği bilinen VDR polimorfizmlerini araştırdık. Gereç ve Yöntem: TaqI (rs731236) ve Fok-I (rs2228570) polimorfizmleri 72 LDH hasta ve 81 sağlıklı kontrol örneğinde polimeraz zincir reaksiyonu- restriksiyon fragman uzunluk polimorfizmi (PCR-RFLP) yöntemi kullanılarak incelendi. Bulgular: Elde edilen bulguların değerlendirilmesi sonrası VDR Taq-I Tt genotipine LDH hastalarında görülme sıklığı kontrollerden anlamlı olarak yüksek olduğu ve Taq-I Tt genotip ve t alel taşıyıcılarının LDH vakaları için yüksek risk taşıyıcısı olduğu tespit edildi, sırasıyla; p=0,002, OR: 1,688,% 95CI: 1,206-2,360; p=0,006, OR: 1,420,% 95CI: 1,104-1,825. VDR Fok-I genotipleri LDH ve kontrol vakaları arasında değerledirildiğinde anlamlı farklılık gözlemlenmemiştir (p=0,079). Ancak, Ff genotipi ve f allel taşıyıcıları LDH hastaları için diğer genotiplere göre daha yüksek bir risk taşımaktadır. Sırasıyla; p=0,025, OR: 1,594, %95 CI: 1,052-2,414; p=0,037, OR: 1,514, %95 CI: 1,019-2,250. Sonuç: Çalışmamız, LDH'li hastaların belirli alt grupları için genetik risk faktörlerinin tanımlanmasına katkıda bulunmaktadır ve genetik biyobelirteçlerin hastaların ayrıntılı klinik değerlendirmesine katkısının önemini vurgulamaktadır. Cite this article as: Toptaş Hekimoğlu B, Eraltan H, Sarı H, Hakan MT, Sönmez D, Horozoğlu C, et al. Investigation of The Vitamin D Receptor (VDR) Gene Polymorphisms in Lumbar Disc Herniation in Turkish Patients. Experimed 2019; 9(3): 93-8.

Keywords

• D vitamini reseptörü,polimorfizm,lomber disk hernisi

Investigation of The Vitamin D Receptor (VDR) Gene Polymorphisms in Lumbar Disc Herniation in Turkish Patients

Abstract

Objective: Lumbar disc herniation (LDH) is a common degenerative disease. It is still not clear if there is a possible association between the vitamin D pathway and the etiopathogenesis of the disease. In this study, we investigated certain VDR polymorphisms which are known to affect vitamin D levels in patients with lumbar disc herniation. Material and Method: TaqI (rs731236) and Fok-I (rs2228570) polymorphisms were studied by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in 72 LDH patients and 81 healthy controls. Results: After evaluation of our results, the frequency of LDH patients who have VDR Taq-I Tt genotype was significantly higher than the controls and carriers of Taq-I Tt genotype and t allele who had an increased risk for lumbar disc hernia cases, respectively p=0.002, OR:1.688, 95%CI:1.206-2.360; p=0.006, OR:1.420, 95%CI:1.104-1.825. VDR Fok-I genotypes did not differ significantly between lumbar disc herniation and control cases. (p=0.079). But, Ff genotype and f allele carriers had a higher risk for lumbar disk hernia than those with other genotypes, respectively p=0.025, OR:1.594, 95%CI:1.052-2.414; p=0.037, OR:1.514, 95%CI:1.019-2.250. Conclusion: Our study contributes to the identification of genetic risk factors for specific subgroups of patients with LDH, and emphasizes the contribution of these biomarkers to the detailed clinical evaluation of patients with genetic biomarkers. Cite this article as: Toptaş Hekimoğlu B, Eraltan H, Sarı H, Hakan MT, Sönmez D, Horozoğlu C, et al. Investigation of The Vitamin D Receptor (VDR) Gene Polymorphisms in Lumbar Disc Herniation in Turkish Patients. Experimed 2019; 9(3): 93-8.

Keywords

• Vitamin D receptor,polymorphism,lumbar disc herniation

References

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