Sodyum Lauril Sülfatın Gelişmekte Olan Zebra Balığı Embriyolarında Biyouyumluluğu

Abstract

Amaç: Sodyum lauril sülfat (SLS), ev temizlik ürünleri ve diş macun-larında emülsifiye edici temizlik maddesi olarak kullanılan anyonik bir yüzey aktif maddedir. Birçok diş macunu fırçalarken köpük oluşumuna yol açan SLS içerir. Ancak SLS hassas diş ve diş etlerini tahriş edebilir. Lipid peroksidasyonu (LPO), çeşitli hastalıkların patofizyo-lojisinde oksidatif strese yol açan serbest radikallerin saldırısı ile başlatılan bir otooksidasyon sürecidir. LPO, LPO'nun biyobelirteçleri olarak görev yapan farklı aldehitler, ketonlar, alkanlar dahil olmak üzere reaktif ürünlerin oluşumuna neden olur. Çalışmamızın amacı, SLS'ye maruz kalan zebra balığı embriyolarında oluşan LPO'yu değerlendirmektir.

Gereç ve Yöntem: Normal olarak bölünen küresel embriyolar, 72 saat boyunca plaka kuyucuklarında düşük ve yüksek dozda SLS'ye maruz bırakılmıştır. Mortalite ve kuluçkadan çıkma oranları belir-lenmiştir. Tiyobarbitürik asit reaktif maddeler olarak LPO'nun son ürünü olan malondialdehit (MDA) düzeyini belirlemek için Yagi yöntemi kullanılmıştır.

Bulgular: Bulgularımız, hem düşük SLS (p<0,05) hem de yüksek doz SLS’ye (p<0,05) maruz kalan zebra balığı embriyolarında kont-rol grubu ile karşılaştırıldığında LPO'nun önemli ölçüde arttığını göstermiştir.

Sonuç: LPO'nun embriyogenez sırasında SLS'ye maruz kalmanın erken bir göstergesi olduğu öne sürülebilir ve bu bulguyu doğru-lamak için farklı çalışmaların yapılması gerektiği düşünülmektedir.

Keywords

• Sodyum lauril sülfat
• diş macunu
• lipit peroksi-dasyon
• zebra balığı embriyosu

The Biocompatibility of Sodium Lauryl Sulphate on Developing Zebrafish Embryos

Abstract

Objective: As an anionic surfactant, sodium lauryl sulphate (SLS), is used as an emulsifying agent in toothpastes as well as many dif-ferent household cleaning products. Many toothpastes contain SLS and it is responsible for the formation of foam when brush-ing. However SLS may also irritate sensitive teeth and gums. Lip-id peroxidation (LPO) is defined as an autoxidation process that is induced through the attack of free oxygen radicals leading to oxidative stress in the pathophysiology of various diseases. LPO causes the formation of highly reactive products including differ-ent aldehydes, ketones, and alkanes serving as biomarkers of LPO. In our study we aimed to expose zebrafish embryos to SLS and de-termine LPO in SLS exposed zebrafish embryos.

Material and Method: Zebrafish embryos that were dividing nor-mally and which had spherical shapes were chosen and they were ex-posed to SLS both in low and high concentrations in well plates for 72 hours. Rates of mortality and hatching were determined. The levels of malondialdehyde were evaluated using the Yagi's method as the end products of LPO in the form of thiobarbituric acid reactive substances.

Results: Our findings showed LPO increased significantly in both low SLS (p<0.05) and high dose SLS (p<0.05) exposed zebrafish embryos when they were compared to the control group.

Conclusion: It may be suggested that LPO is an early indicator of exposure to SLS during embryogenesis and further studies are re-quired to confirm this finding.

Keywords

• Sodium lauryl sulphate
• toothpaste
• lipid peroxidation
• zebrafish embryos

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