Antimicrobial Activity of Pulicaria Species from Turkey

Year 2021, , 195 - 199, 08.12.2021

Ebru Özdemir Nath , Merve Bilgin Bahar Gurdal

https://doi.org/10.26650/experimed.2021.986570

Abstract

Objective: In traditional medicine, the Pulicaria species are used for various disorders such as inflamed wounds, skin diseases, and bronchitis. This study investigated the antibacterial effect of five Pulicaria species in Turkey; (Pulicaria (P) arabica (L.) Cass., P. dysen-terica (L.) Bernh., P. odora (L.) Reichb., P. sicula (L.) Moris, P. vulgaris (L.) Gaertn.) against certain significant pathogenic gram-negative and gram-positive reference bacteria.

Material and Method: Four extracts (decoction, infusion, aque-ous, and ethanol (EtOH) extracts) were prepared from the Pulicariaspecies. The antimicrobial activity of the samples was examined against reference organisms; Bacillus (B) subtilis, Enterococcus fae-calis, Escherichia coli, Klebsiella pneumoniae, Methicillin-resistant Staphylococcus (S) aureus (MRSA), Proteus mirabilis, S. aureus andPseudomonas aeruginosa. The minimal bactericidal concentration (MBC) and minimum inhibitory concentration (MIC) ranges of the extract samples were demonstrated based on a microbroth dilu-tion method.

Results: The EtOH extracts of the studied four Pulicaria species were found to be weakly active against gram-positive bacteria such as B. subtilis, MRSA and S. aureus but the EtOH extract of P. dy-senterica showed exceptionally good activity against the reference strains of S. aureus, MRSA. No antimicrobial activity was detected in the infusion, decoction, and aqueous extracts.

Conclusion: The Pulicaria species, especially P. dysenterica could be evaluated as antimicrobial agents. Further studies with the extracts and essential oils from Pulicaria sp. on other bacteria and pathogenic fungi should be performed.

Keywords

Pulicaria, antimicrobial activity, extracts, Turkey

Supporting Institution

This study was funded by the Scientific Research Projects Coordination Unit of Istanbul University.

Project Number

Project number: 29555.

Thanks

The authors thank Scientific Research Projects Coordination Unit of Istanbul University and DÜAGEM (Altınbaş University Natural Products Research and Development Center).

Türkiye’deki Pulicaria Türlerinin Antimikrobiyal Aktivitesi

Abstract

Amaç: Geleneksel tıpta Pulicaria türleri iltihaplı yaralar, cilt hastalık-ları, bronşit gibi çeşitli rahatsızlıklarda kullanılmaktadır. Bu çalışma-da Türkiye'deki beş Pulicaria türünün (Pulicaria (P) arabica (L.) Cass., P. dysenterica (L.) Bernh., P. odora (L.) Reichb., P. sicula (L.) Moris, P. vul-garis (L.) Gaertn.), bazı önemli patojenik gram-negatif ve gram-po-zitif referans bakterilere karşı etkisi araştırılmıştır.

Gereç ve Yöntem:Pulicaria türlerinden dört ekstre (kaynatma, in-füzyon, sulu ve etanol (EtOH) ekstreleri) hazırlandı. Örneklerin, anti-mikrobiyal aktivitesi referans organizmalara karşı incelendi; Bacillus (B) subtilis, Enterococcus faecalis, Escherichia coli, Klebsiella pneumo-niae, Metisiline dirençli Staphylococcus (S) aureus (MRSA), Proteus mirabilis, S. aureus ve Pseudomonas aeruginosa. Ekstre numuneleri-nin minimum bakterisidal konsantrasyon (MBC) ve minimum inhi-bitör konsantrasyon (MIC ) aralıkları, mikrobroth seyreltme yöntemi temel alınarak gösterilmiştir.

Bulgular: İncelenen dört Pulicaria türünün EtOH ekstrelerinin; B. subtilis, MRSA ve S. aureus gibi gram pozitif bakterilere karşı genel-likle zayıf aktif olduğu bulunmuştur, ancak P. dysenterica'nın EtOH ekstresi, S. aureus, MRSA referans suşlarına karşı çok iyi aktivite gös-termiştir. İnfüzyon, dekoksiyon ve sulu ekstrelerde antimikrobiyal aktivite tespit edilememiştir.

Sonuç:Pulicaria türleri, özellikle P. dysenterica antimikrobiyal ajan olarak değerlendirilebilir. Pulicaria sp.'den elde edilen ekstreler ve uçucu yağlar ile diğer bakteri ve bazı patojenik mantarlar üzerinde daha ileri çalışmalar yapılmalıdır.

Keywords

Pulicaria, antimikrobiyal aktivite, ekstreler, Türkiye

Project Number

Project number: 29555.

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