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Effect of Deuterium Depletion on Erythrocytes in Experimental Diabetes

Year 2020, Volume: 10 Issue: 1, 30 - 37, 13.03.2020
https://doi.org/10.26650/experimed.2020.0007

Abstract

In the development of diabetic complications, oxidative stress caused by hyperglycemia plays an important role. Deuterium depletion has protective effect against cellular damage and tumor suppressor effect in cancer cell lines. The purpose of this study was to examine and evaluate the effect of deuterium depletion on erythrocyte oxidant and antioxidant system parameters in experimental diabetes induced by streptozotocin (STZ) in rats. On the 30th or 40th day after STZ injection, blood samples were taken and blood glucose levels were measured. Erythrocyte hemolysates were also prepared. Lipid peroxidation and glutathione levels and glutathione-S-transferase, superoxide dismutase and catalase enzyme activities of erythrocytes were determined. At the 30 or 40 day periods, deuterium depleted water (DDW) consumption had differently affected oxidant-antioxidant parameters of erythrocytes. The clear effect of deuterium depletion in drinking water from 150 ppm to 85 ppm was observed in glutathione levels and it increased erythrocyte glutathione levels in both the control and diabetic groups. In conclusion, deuterium depletion may have protective effects on diabetes induced oxidative stress. Further studies are needed to be done to determine the mechanism of action and the useful dosage and the application period of DDW.

Supporting Institution

This study was supported by a grant from Scientific Research Project Department of Marmara University (Project No: SAĞ-C-YLP-171108-0261).

Project Number

Project No: SAĞ-C-YLP-171108-0261

References

  • 1. Collins CJ, Bowman NS (Eds). Effects In Chemical Reactions. Van Nostrand Reinhold, York, pp. 286-363, 1971.
  • 2. Wiberg KB. The Deuterium Isotope Effect. Chem Rev. 1955, 55: 713-43. [CrossRef]
  • 3. Jancso G, Van Hook WA. Condensed Phase Isotope Effects: Especially Vapor Pressure. Isotope Effects 1974; 74: 689-750. [CrossRef]
  • 4. Rundel PW, Ehleringer JR, Nagy KA: Stable In Ecological Research. Springer, York, pp. 7-9, 1988. [CrossRef]
  • 5. Blaga L. The Deuterium content of human metabolic fluids in relation with human metabolic processes (in Romania), IFA-Bucuresti, Scientific Report, 1978, 1-4.
  • 6. Berdea P, Cuna S, Cazacu M, Tudose M. Deuterium Variation of Human Blood Serum. Studia Universitatis Babeş-Bolyai, Physica, Special Issue 2001; 256-8.
  • 7. Somlyai G, Jancso G, Jakli G, Vass K, Barna B, Lakics V, Gaal T. Naturally occuring deuterium is essential for the normal growth rate of cells. FEBS Letters 1993; 317: 1-4. [CrossRef]
  • 8. Somlyai G. Defeating Cancer. The Biological Effect of Deuterium Depletion by Gabor Somlyai HYD Ltd. for Research and Development ISBN: 0-7596-9261-0;2002.
  • 9. Somlyai G, Laskay G, Berkenyi T, Galbacs Z, Galbacks G, Kiss SA, Jakli GY, Jancso G. The Biological Effects of Deuterium-Depleted Water, a Possible New Tool in Cancer Therapy. Journal of Oncology 1998; 30(4): 91-4.135.
  • 10. Cong F, Zhang Y, Sheng H, Ao Z, Zhang S, Wang J. Deuterium-depleted water inhibits human lung carcinoma cell growth by apoptosis. Experimental and Therapeutic Medicine 2010; 1: 277-83. [CrossRef]
  • 11. Somlyai G, Molnar M, Laskay G, Szabo M, Berkenyi T, Guller I, Kovaks A. Biological significance of naturally occurring deuterium: the antitumor effect of deuterium depletion. Orvosi Hetilap 2010; 5; 151(36): 1455-60. [CrossRef]
  • 12. Keempels K, Somlyi I, Somlyai G. A retrospective evaluation of the effects of deuterium depleted water consumption on 4 patients with brain metastases from lung cancer. Integrative Cancer Therapies 2008; 7(3): 172-81. [CrossRef]
  • 13. Katz JJ, Crespi HL, Hasterlik RJ, Thomson JF, Finkel AJ. Some Observations on Biological Effects of Deuterium, with Special Reference to Effects on Neoplastic Processes. Journal of the National Cancer Institute 1957; 18(5): 641-58.
  • 14. Synergistic Effects of Deuterium Oxide and Gemcitabine in Human Pancreatic Cancer Cell Line, Cancer Letter 2008; 259: 231-9. [CrossRef]
  • 15. Bahk JY, Lee JH, Chung HS, LeeHY, Chung BC, Park MS, Min SK, Kim MO. Anticancer Effect of Deuterium Oxide on a Bladder Cancer Cell Related to Bcl-2 and Bax. Journal of Indian Engineering Chemistry 2007; 13(4): 501-7.
  • 16. MaritimAC, Sanders RA, Watkins JB. Diabetes, Oxidative Stress and Antioxidants: A Review. Journal of Biochemical and Molecular Toxicology 2003; 17(1): 24-38. [CrossRef]
  • 17. Nwose EU, Jelinek HF, Richards RS, Kerr PG. Erythrocyte oxidative stress in clinical management of diabetes and its cardiovascular complications. British Journal of Biomedical Sciences 2007; 64(1): 35-43. [CrossRef]
  • 18. Shin S, Ku Y, Babu N, Singh M. Erythrocyte deformability and its variation in diabetes mellitus. Indian Journal of Experimental Biology 2007; 45(1): 121-8.
  • 19. Leoncini S, Rossi V, Signorini C, Tanganelli I, Comporti M, Ciccoli L. Oxidative stress, erythrocyte ageing and plasma non-proteinbound iron in diabetic patients. Free Radical Research 2008; 42(8): 716-4. [CrossRef]
  • 20. Alekseevich TA et al. Method for treatment of patients with diabetes mellitus, 01-10(ru2270017), 2006.
  • 21. Tatsuo U, Kunihiro S. Treatment of Cancer, Diabetes Mellitus, Leukemia, Aplastic Anemia and Insomnia, Treatment and Prevention of Severe Acute Respıratory Syndrome (Sars), Rejuvenation, Activation of Cell and Promotion of Increase of Immune Cell by Soy Sauce Prepared by Using Deuterium-Attrited Water (Super Light Water) as Raw Material, and Method for Producing The Same. Internatl Scient KK. A23L1/238; 12-24 (JP2004357678), 2004.
  • 22. Habig WH, Jacoby WB. Assays for differentiation of glutathione-s- transferases. Methods in Enzymology 1981; 77: 398-405. [CrossRef]
  • 23. Aebi H.: Catalase invitro. In: Methods of enzymatic Analysis, 2nd Edition, vol.2, Bergmeyer HU (Ed), FL., pp.121-126.
  • 24. Mylorie AA, Collins H, Umbles C, Kyle J. Erythrocyte superoxide dismutase activity and other parameters of cupper status in rats ingesting lead acetate. Toxicology and Applied Pharmacology 1986; 82: 512-520. [CrossRef]
  • 25. Ledwozyw A, Michalak D, Stepien A, Kadziolka A. The relationship between plasma triglycerides, cholesterol, total lipids and lipid peroxidation products during human atherosclerosis. Clinica Chimica Acta 1986; 155; 275-83. [CrossRef]
  • 26. Beutler E.: Glutathione in Red Cell Metabolism: A manual of Biochemical methods, 2nd ed.: Grune and Stratton, NY., pp.112-114.
  • 27. Watkins PJ. ABC of Diabetes. 5th ed, BMJ Publishing Group Ltd, London, p.2-3,2003.
  • 28. Pietropaolo M, Trucco M.Genetics of Type I Diabetes. In: Type 1 Diabetes. Ed: Sperling MA, Humana Press Inc, New Jersey, p.28, 2003.
  • 29. Yarat A, Yanardag R, Tunalı T, Sacan O, Gursoy F, Emekli N, Ustuner A, Ergenekon G. Effects of glibornuride versus metformin on eye lenses and skin in experimental diabetes. Arzneimittel Forschung Drug Research 2006; 56(7): 541-6. [CrossRef]
  • 30. Szkudelski T. The mechanism of alloxan and streptozotocin action in B cells of the rat pancreas. Physiological Research 2001; 50: 53646.
  • 31. Junod A, Lambert AE, Stauffacher W, Renold AE. Diabetogenic action of streptozotocin: Relation of dose to metabolic response. The Journal of Clinical Investigation 1969; 48: 2129-39. [CrossRef]
  • 32. Tatsuki R, Satoh K, Yamamoto A, Hoshi K, Ichihara K. Lipid peroxidation in the pancreas and other organs in streptozotocin diabetic rats. Japanese Journal Pharmacology 1997; 75: 267-73. [CrossRef]
  • 33. Coşkun O, Ocakcı A, Bayraktaroğlu T, Kanter M. Exercise training prevents and protects streptozotocin-induced oxidative stress and β- cell damage in rat pancreas. Tohoku Journal Experimental Medicine 2004; 203: 145-54. [CrossRef]
  • 34. Atkinson MA, Eisenbarth GS. Type 1 diabetes: new perspectives on disease pathogenesis and treatment. Lancet 2001; 358: 221-9. [CrossRef]
  • 35. Griesmacher A, Kindhauser M, Andert SE, Schreiner W, Tomo C, Kroebl P, Pietschmann P, Prager R, Schnack C, Schernthaner G, Mueller M. Enhanced Serum levels of thiobarbituric acid - reactive substances in diabetes mellitus. American Journal of Medicine 1995; 98(5): 469-75. [CrossRef]
  • 36. Giugliano D, Ceriello A, Paolisso G. Oxidative stress and diabetic vascular complications. Diabetes Care 1996; 19: 257-67. [CrossRef]
  • 37. Qujeq D, Rezvani T. Catalase (antioxidant enzyme) activity in streptozotocin-induced diabetic rats. International Journal of Diabetes and Metabolism 2007; 15: 22-4.
  • 38. Sekar N, Kanthasamy A, William S, Balasubramaniyan N, Govindasamy S. Antioxidant effect of vanadate on experimental diabetic rats. Acta Diabetologica Latina 1990; 27: 285-93. [CrossRef]
  • 39. Mukherjee B, Mukherjee JR, Chatterjee M. Lipid peroxidation, glutathione levels and changes in glutathione-related enzyme activities in streptozotocin-induced diabetic rats. Immunology and Cell Biology 1994; 72: 109-14. [CrossRef]
  • 40. Ramakrishna V, Jailkhani R. Evalution of oxidative stress in insulin dependent diabetes mellitus (IDDM) patients. Diagnostic Pathology 2007; 2: 22. [CrossRef]
  • 41. Peuchant E, Brun JL, Rigalleau V, Dubourg L, Thomas MJ, Daniel JY, Leng JJ, Gin H. Oxidative and antioxidative status in pregnant women with either gestational or type 1 diabetes. Clinical Biochemistry 2004; 37: 293-8. [CrossRef]
  • 42. Bhatia S, Shukla R, Madhu SR, Gambhir JK, Prabhu KM. Antioxidant status, lipid peroxidation and nitric oxide end product in patients of type 2 diabetes mellitus with nephropathy. Clinical Biochemistry 2003; 36: 557-62. [CrossRef]
  • 43. Memişoğulları R, Taysı S, Bakan E, Capoğlu I. Antioxidant status and lipid peroxidation in type II diabetes mellitus. Cellular Biochemical Function 2003; 21: 291-6. [CrossRef]
  • 44. Sailaja YR, Baskar R, Saralakumari D. Antioxidant status during maturation of reticulocytes to erythrocytes in type 2 diabetics. Free Radical Biology and Medicine 2003; 35(2): 133-9. [CrossRef]
  • 45. Vural H, Sabuncu T, Arslan SO, Aksoy N. Melatonin inhibits lipid peroxidation and stimulates the antioxidant status of diabetic rats. Journal of Pineal Research 2001; 31: 193-8. [CrossRef]
  • 46. Chandramohan G, Al-Numair KS, Pugalendi KV. Restoration of altered plasma, erythrocyte and liver antioxidant levels. International Journal of Integrative Biology 2009; 5(3): 176-81.
  • 47. Bild W, Nastasa V, Haulica I. In vivo and in vitro research on biological effects of deuterium-depleted water: 1. Influence of deuterium-deoleted water on cultured cell growth. Romanian Journal of Physiology 2004; 41(1-2): 53-67.
  • 48. Haulica I, Peculea M, Stefanescu I, Titescu G, Todiras M, Bild W. Effects of heavy and deuterium-depleted water on vascular reactivity. Romanian Journal of Physiology 1998; 35(1-2): 25-32.
  • 49. Bild W, Stefanescu I, Haulica I, Lupusoru C, Titescu G, Iliescu R, Nastas V. Research concerning the radioprotective and immunostimulating effects of deuterium depleted water. Romanian Journal of Physiology 1999; 36(3-4): 205-18.
  • 50. Loike JD, Caol L, Kuang K, Vera JC, Silverstein SC, Fischbarg J. Role of facilitative glucose transporters in diffusional water permeability through J774 cells. Journal of Genetic Physiology 1993; 102(5): 897-906. [CrossRef]
  • 51. Kovács A, Guller I, Krempels K, Somlyai I, Jánosi I, Gyomgyi Z, Szabó I, Ember IGS (2011) Deuterium depletion may delay the progression of prostate cancer. J Cancer Ther 2011; 2: 548-56. [CrossRef]
  • 52. Ávila DS, Somlyai G, Somlyai I, Aschner M. Anti-aging effects of deuterium depletion on Mn-induced toxicity in a C. Elegans Model. Toxicol Lett 2012; 211: 319-24. [CrossRef]
  • 53. Gyöngyi Z, Budan F, Szabo I, Ember I, Kiss I, Krempels K, Somlyai I, Somlyai G. Deuterium depleted water effects on survival of lung cancer patients and expression of Kras, Bcl2, and Myc genes in Mouse lung. Nutr Cancer 2013; 65(2): 240-6. [CrossRef]
  • 54. Mladin C, Ciobica A, Lefter R, Popescu A, Bild W. Deuterium depleted water has stimulating effects on long-term memory in rats. Neurosci Lett 2014; 583: 154-8. [CrossRef]
  • 55. Soleyman-Jahi S, Zendehdel K, Akbarzahed K, Haddadi M, Amanpour S, Muhammadnejad S. In vitro Assessment of antineoplastik effects of döteryum depleted water. Asian Pac J Cancer Prev 2014; 15(5): 2179-83. [CrossRef]
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  • 58. Boros LG, D'Agostino DP, Katz HE, Roth JP, Meuillet EJ, Somlyai G. Submolecular regulation of cell transformation by deuterium depleting water exchange reactions in the tricarboxylic acid substrate cycle. Med Hypothesis 2016; 87: 69-74. [CrossRef]
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Deneysel Diyabette Döteryum Azlığının Eritrositler Üzerine Etkisi

Year 2020, Volume: 10 Issue: 1, 30 - 37, 13.03.2020
https://doi.org/10.26650/experimed.2020.0007

Abstract

Diabetik komplikasyonların gelişmesinde, hiperglisemi kaynaklı oksidatif stres çok önemli bir rol oynamaktadır. Döteryum azlığının hücresel hasara karşı ve tümör baskılayıcı etki üzerine koruyucu etkisi olduğu kanser hücre hatlarında gösterilmiştir. Çalışmanın amacı, döteryum azaltılmasının sıçanlarda streptozotosin (STZ) ile oluşturulmuş deneysel diabette eritrosit oksidan ve antioksidan sistem parametreleri üzerindeki etkisinin araştırılması ve değerlendirilmesidir. STZ enjeksiyonunu takiben 30. veya 40. günde, kan örnekleri alınıp, kan glukoz değerleri ölçülmüş, eritrosit hemolizatları da hazırlanmıştır. Eritrositlerin lipid peroksidasyon ve glutatyon seviyeleri ile glutatyon-S-transferaz, süperoksit dismutaz ve katalaz enzim aktiviteleri tayin edilmiştir. 30 veya 40 günlük periyotlardaki döteryumu azaltılmış su tüketiminin, eritrosit oksidan-antioksidan parametre düzeylerine etkisinin farklı olduğu görülmüştür. İçme suyunda döteryumun 150 ppm den 85 ppm’e azaltılmasının net etkisi glutatyon düzeylerinde gözlenmiş olup hem kontrol hem de diyabetik gruplarda eritrosit glutatyon düzeylerini artırdığı saptanmıştır. Sonuç olarak, döteryumu azaltılmış suyun, diabete bağlı oksidatif hasara karşı koruyucu etkisi olabilir. Döteryumu azaltılmış suyun etki mekanizmasının, uygun dozunun ve uygulama süresinin aydınlatılması için daha fazla çalışmalara ihtiyaç vardır.

Project Number

Project No: SAĞ-C-YLP-171108-0261

References

  • 1. Collins CJ, Bowman NS (Eds). Effects In Chemical Reactions. Van Nostrand Reinhold, York, pp. 286-363, 1971.
  • 2. Wiberg KB. The Deuterium Isotope Effect. Chem Rev. 1955, 55: 713-43. [CrossRef]
  • 3. Jancso G, Van Hook WA. Condensed Phase Isotope Effects: Especially Vapor Pressure. Isotope Effects 1974; 74: 689-750. [CrossRef]
  • 4. Rundel PW, Ehleringer JR, Nagy KA: Stable In Ecological Research. Springer, York, pp. 7-9, 1988. [CrossRef]
  • 5. Blaga L. The Deuterium content of human metabolic fluids in relation with human metabolic processes (in Romania), IFA-Bucuresti, Scientific Report, 1978, 1-4.
  • 6. Berdea P, Cuna S, Cazacu M, Tudose M. Deuterium Variation of Human Blood Serum. Studia Universitatis Babeş-Bolyai, Physica, Special Issue 2001; 256-8.
  • 7. Somlyai G, Jancso G, Jakli G, Vass K, Barna B, Lakics V, Gaal T. Naturally occuring deuterium is essential for the normal growth rate of cells. FEBS Letters 1993; 317: 1-4. [CrossRef]
  • 8. Somlyai G. Defeating Cancer. The Biological Effect of Deuterium Depletion by Gabor Somlyai HYD Ltd. for Research and Development ISBN: 0-7596-9261-0;2002.
  • 9. Somlyai G, Laskay G, Berkenyi T, Galbacs Z, Galbacks G, Kiss SA, Jakli GY, Jancso G. The Biological Effects of Deuterium-Depleted Water, a Possible New Tool in Cancer Therapy. Journal of Oncology 1998; 30(4): 91-4.135.
  • 10. Cong F, Zhang Y, Sheng H, Ao Z, Zhang S, Wang J. Deuterium-depleted water inhibits human lung carcinoma cell growth by apoptosis. Experimental and Therapeutic Medicine 2010; 1: 277-83. [CrossRef]
  • 11. Somlyai G, Molnar M, Laskay G, Szabo M, Berkenyi T, Guller I, Kovaks A. Biological significance of naturally occurring deuterium: the antitumor effect of deuterium depletion. Orvosi Hetilap 2010; 5; 151(36): 1455-60. [CrossRef]
  • 12. Keempels K, Somlyi I, Somlyai G. A retrospective evaluation of the effects of deuterium depleted water consumption on 4 patients with brain metastases from lung cancer. Integrative Cancer Therapies 2008; 7(3): 172-81. [CrossRef]
  • 13. Katz JJ, Crespi HL, Hasterlik RJ, Thomson JF, Finkel AJ. Some Observations on Biological Effects of Deuterium, with Special Reference to Effects on Neoplastic Processes. Journal of the National Cancer Institute 1957; 18(5): 641-58.
  • 14. Synergistic Effects of Deuterium Oxide and Gemcitabine in Human Pancreatic Cancer Cell Line, Cancer Letter 2008; 259: 231-9. [CrossRef]
  • 15. Bahk JY, Lee JH, Chung HS, LeeHY, Chung BC, Park MS, Min SK, Kim MO. Anticancer Effect of Deuterium Oxide on a Bladder Cancer Cell Related to Bcl-2 and Bax. Journal of Indian Engineering Chemistry 2007; 13(4): 501-7.
  • 16. MaritimAC, Sanders RA, Watkins JB. Diabetes, Oxidative Stress and Antioxidants: A Review. Journal of Biochemical and Molecular Toxicology 2003; 17(1): 24-38. [CrossRef]
  • 17. Nwose EU, Jelinek HF, Richards RS, Kerr PG. Erythrocyte oxidative stress in clinical management of diabetes and its cardiovascular complications. British Journal of Biomedical Sciences 2007; 64(1): 35-43. [CrossRef]
  • 18. Shin S, Ku Y, Babu N, Singh M. Erythrocyte deformability and its variation in diabetes mellitus. Indian Journal of Experimental Biology 2007; 45(1): 121-8.
  • 19. Leoncini S, Rossi V, Signorini C, Tanganelli I, Comporti M, Ciccoli L. Oxidative stress, erythrocyte ageing and plasma non-proteinbound iron in diabetic patients. Free Radical Research 2008; 42(8): 716-4. [CrossRef]
  • 20. Alekseevich TA et al. Method for treatment of patients with diabetes mellitus, 01-10(ru2270017), 2006.
  • 21. Tatsuo U, Kunihiro S. Treatment of Cancer, Diabetes Mellitus, Leukemia, Aplastic Anemia and Insomnia, Treatment and Prevention of Severe Acute Respıratory Syndrome (Sars), Rejuvenation, Activation of Cell and Promotion of Increase of Immune Cell by Soy Sauce Prepared by Using Deuterium-Attrited Water (Super Light Water) as Raw Material, and Method for Producing The Same. Internatl Scient KK. A23L1/238; 12-24 (JP2004357678), 2004.
  • 22. Habig WH, Jacoby WB. Assays for differentiation of glutathione-s- transferases. Methods in Enzymology 1981; 77: 398-405. [CrossRef]
  • 23. Aebi H.: Catalase invitro. In: Methods of enzymatic Analysis, 2nd Edition, vol.2, Bergmeyer HU (Ed), FL., pp.121-126.
  • 24. Mylorie AA, Collins H, Umbles C, Kyle J. Erythrocyte superoxide dismutase activity and other parameters of cupper status in rats ingesting lead acetate. Toxicology and Applied Pharmacology 1986; 82: 512-520. [CrossRef]
  • 25. Ledwozyw A, Michalak D, Stepien A, Kadziolka A. The relationship between plasma triglycerides, cholesterol, total lipids and lipid peroxidation products during human atherosclerosis. Clinica Chimica Acta 1986; 155; 275-83. [CrossRef]
  • 26. Beutler E.: Glutathione in Red Cell Metabolism: A manual of Biochemical methods, 2nd ed.: Grune and Stratton, NY., pp.112-114.
  • 27. Watkins PJ. ABC of Diabetes. 5th ed, BMJ Publishing Group Ltd, London, p.2-3,2003.
  • 28. Pietropaolo M, Trucco M.Genetics of Type I Diabetes. In: Type 1 Diabetes. Ed: Sperling MA, Humana Press Inc, New Jersey, p.28, 2003.
  • 29. Yarat A, Yanardag R, Tunalı T, Sacan O, Gursoy F, Emekli N, Ustuner A, Ergenekon G. Effects of glibornuride versus metformin on eye lenses and skin in experimental diabetes. Arzneimittel Forschung Drug Research 2006; 56(7): 541-6. [CrossRef]
  • 30. Szkudelski T. The mechanism of alloxan and streptozotocin action in B cells of the rat pancreas. Physiological Research 2001; 50: 53646.
  • 31. Junod A, Lambert AE, Stauffacher W, Renold AE. Diabetogenic action of streptozotocin: Relation of dose to metabolic response. The Journal of Clinical Investigation 1969; 48: 2129-39. [CrossRef]
  • 32. Tatsuki R, Satoh K, Yamamoto A, Hoshi K, Ichihara K. Lipid peroxidation in the pancreas and other organs in streptozotocin diabetic rats. Japanese Journal Pharmacology 1997; 75: 267-73. [CrossRef]
  • 33. Coşkun O, Ocakcı A, Bayraktaroğlu T, Kanter M. Exercise training prevents and protects streptozotocin-induced oxidative stress and β- cell damage in rat pancreas. Tohoku Journal Experimental Medicine 2004; 203: 145-54. [CrossRef]
  • 34. Atkinson MA, Eisenbarth GS. Type 1 diabetes: new perspectives on disease pathogenesis and treatment. Lancet 2001; 358: 221-9. [CrossRef]
  • 35. Griesmacher A, Kindhauser M, Andert SE, Schreiner W, Tomo C, Kroebl P, Pietschmann P, Prager R, Schnack C, Schernthaner G, Mueller M. Enhanced Serum levels of thiobarbituric acid - reactive substances in diabetes mellitus. American Journal of Medicine 1995; 98(5): 469-75. [CrossRef]
  • 36. Giugliano D, Ceriello A, Paolisso G. Oxidative stress and diabetic vascular complications. Diabetes Care 1996; 19: 257-67. [CrossRef]
  • 37. Qujeq D, Rezvani T. Catalase (antioxidant enzyme) activity in streptozotocin-induced diabetic rats. International Journal of Diabetes and Metabolism 2007; 15: 22-4.
  • 38. Sekar N, Kanthasamy A, William S, Balasubramaniyan N, Govindasamy S. Antioxidant effect of vanadate on experimental diabetic rats. Acta Diabetologica Latina 1990; 27: 285-93. [CrossRef]
  • 39. Mukherjee B, Mukherjee JR, Chatterjee M. Lipid peroxidation, glutathione levels and changes in glutathione-related enzyme activities in streptozotocin-induced diabetic rats. Immunology and Cell Biology 1994; 72: 109-14. [CrossRef]
  • 40. Ramakrishna V, Jailkhani R. Evalution of oxidative stress in insulin dependent diabetes mellitus (IDDM) patients. Diagnostic Pathology 2007; 2: 22. [CrossRef]
  • 41. Peuchant E, Brun JL, Rigalleau V, Dubourg L, Thomas MJ, Daniel JY, Leng JJ, Gin H. Oxidative and antioxidative status in pregnant women with either gestational or type 1 diabetes. Clinical Biochemistry 2004; 37: 293-8. [CrossRef]
  • 42. Bhatia S, Shukla R, Madhu SR, Gambhir JK, Prabhu KM. Antioxidant status, lipid peroxidation and nitric oxide end product in patients of type 2 diabetes mellitus with nephropathy. Clinical Biochemistry 2003; 36: 557-62. [CrossRef]
  • 43. Memişoğulları R, Taysı S, Bakan E, Capoğlu I. Antioxidant status and lipid peroxidation in type II diabetes mellitus. Cellular Biochemical Function 2003; 21: 291-6. [CrossRef]
  • 44. Sailaja YR, Baskar R, Saralakumari D. Antioxidant status during maturation of reticulocytes to erythrocytes in type 2 diabetics. Free Radical Biology and Medicine 2003; 35(2): 133-9. [CrossRef]
  • 45. Vural H, Sabuncu T, Arslan SO, Aksoy N. Melatonin inhibits lipid peroxidation and stimulates the antioxidant status of diabetic rats. Journal of Pineal Research 2001; 31: 193-8. [CrossRef]
  • 46. Chandramohan G, Al-Numair KS, Pugalendi KV. Restoration of altered plasma, erythrocyte and liver antioxidant levels. International Journal of Integrative Biology 2009; 5(3): 176-81.
  • 47. Bild W, Nastasa V, Haulica I. In vivo and in vitro research on biological effects of deuterium-depleted water: 1. Influence of deuterium-deoleted water on cultured cell growth. Romanian Journal of Physiology 2004; 41(1-2): 53-67.
  • 48. Haulica I, Peculea M, Stefanescu I, Titescu G, Todiras M, Bild W. Effects of heavy and deuterium-depleted water on vascular reactivity. Romanian Journal of Physiology 1998; 35(1-2): 25-32.
  • 49. Bild W, Stefanescu I, Haulica I, Lupusoru C, Titescu G, Iliescu R, Nastas V. Research concerning the radioprotective and immunostimulating effects of deuterium depleted water. Romanian Journal of Physiology 1999; 36(3-4): 205-18.
  • 50. Loike JD, Caol L, Kuang K, Vera JC, Silverstein SC, Fischbarg J. Role of facilitative glucose transporters in diffusional water permeability through J774 cells. Journal of Genetic Physiology 1993; 102(5): 897-906. [CrossRef]
  • 51. Kovács A, Guller I, Krempels K, Somlyai I, Jánosi I, Gyomgyi Z, Szabó I, Ember IGS (2011) Deuterium depletion may delay the progression of prostate cancer. J Cancer Ther 2011; 2: 548-56. [CrossRef]
  • 52. Ávila DS, Somlyai G, Somlyai I, Aschner M. Anti-aging effects of deuterium depletion on Mn-induced toxicity in a C. Elegans Model. Toxicol Lett 2012; 211: 319-24. [CrossRef]
  • 53. Gyöngyi Z, Budan F, Szabo I, Ember I, Kiss I, Krempels K, Somlyai I, Somlyai G. Deuterium depleted water effects on survival of lung cancer patients and expression of Kras, Bcl2, and Myc genes in Mouse lung. Nutr Cancer 2013; 65(2): 240-6. [CrossRef]
  • 54. Mladin C, Ciobica A, Lefter R, Popescu A, Bild W. Deuterium depleted water has stimulating effects on long-term memory in rats. Neurosci Lett 2014; 583: 154-8. [CrossRef]
  • 55. Soleyman-Jahi S, Zendehdel K, Akbarzahed K, Haddadi M, Amanpour S, Muhammadnejad S. In vitro Assessment of antineoplastik effects of döteryum depleted water. Asian Pac J Cancer Prev 2014; 15(5): 2179-83. [CrossRef]
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There are 59 citations in total.

Details

Primary Language English
Subjects Clinical Sciences
Journal Section Research Article
Authors

Arif Çolak This is me

Ayşen Yarat This is me 0000-0002-8258-6118

Project Number Project No: SAĞ-C-YLP-171108-0261
Publication Date March 13, 2020
Submission Date March 5, 2020
Published in Issue Year 2020 Volume: 10 Issue: 1

Cite

Vancouver Çolak A, Yarat A. Effect of Deuterium Depletion on Erythrocytes in Experimental Diabetes. Experimed. 2020;10(1):30-7.