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Lomber Dejeneratif Disk Hastalığında Glutatyon Peroksidaz (GSH-Px) Düzeylerinin Prognostik Önemi

Year 2020, Volume: 10 Issue: 2, 67 - 71, 26.08.2020
https://doi.org/10.26650/experimed.2020.0011

Abstract

Amaç: Bu çalışmanın temel amacı, yaş nedeniyle disk dejenerasyonu potansiyeli olan bir popülasyonda, azalmış antioksidan aktivitesinin intervertebral disk dejenerasyonu ile ilişkisini araştırmaktır.
Gereç ve Yöntem: Çalışmaya lomber disk dejenerasyonu (LDD) tanısı almış 39 hasta ve 37 sağlıklı gönüllü dahil edildi. Hasta verileri yaş, semptomlar, nörolojik muayene bulguları, lomber MRG bulguları, Oswestry Skala (ODI) skorları ve Visüel Analog Skala (VAS) kullanıldı. İnsan Glutatyon peroksidaz (GSH-Px) seviyeleri Enzime Bağlı İmmünosorbent Ölçüm (ELISA) yöntemi ile tespit edildi.
Bulgular: Serum GSH-Px düzeylerinin LDD tanısı almış hasta grubunda sağlıklı kontrollere göre anlamlı olarak düşük olduğu belirlendi (p=0,011).
Sonuç: Çalışmamızda çevresel faktörlere ek olarak GSH-Px enzim eksikliği ile lomber disk dejenerasyonu arasında bir korelasyon olduğu tespit edilmiştir.

Supporting Institution

Bu çalışma Yeditepe Üniversitesi Proje Yönetim Ofisi tarafından desteklenmiştir

References

  • 1. Miller JA, Schmatz C, Schultz AB. Lumbar disc degeneration: correlation with age, sex, and spine level in 600 autopsy specimens. Spine (Phila Pa 1976) 1988; 13: 173-8. [CrossRef] 2. Yuceli S. Facet Cysts. JTSS 2018; 29: 219-21.
  • 3. Prescher A. Anatomy and pathology of the aging spine. Eur J Radiol 1998; 27: 181-95. [CrossRef]
  • 4. Edelson JG, Nathan H. Stages in the natural history of the vertebral end-plates. Spine (Phila Pa 1976) 1988; 13: 21-6. [CrossRef]
  • 5. Maatta JH, Wadge S, MacGregor A, Karppinen J, Williams FM. ISSLS Prize Winner: Vertebral Endplate (Modic) Change is an Independent Risk Factor for Episodes of Severe and Disabling Low Back Pain. Spine (Phila Pa 1976) 2015; 40: 1187-93. [CrossRef]
  • 6. Antar V, Baran O, Yuceli S, Erdogan H, Altintas O, Baran GE, et al. Assessment of the neuroprotective effects of the acetylcholinesterase inhibitor huperzine A in an experimental spinal cord trauma model. J Neurosurg Sci 2018; 62: 128-39.
  • 7. Cecen DA, Tatarli N, Turan Suslu H, Ozdogan S, Barisik NO. Primary Dural Spinal Lymphoma Presentation of a Rare Spinal Tumor Case. Case Rep Surg 2015; 639253. [CrossRef]
  • 8. Kazanci B, Ozdogan S, Kahveci R, Gokce EC, Yigitkanli K, Gokce A, et al. Neuroprotective Effects of Pregabalin Against Spinal Cord Ischemia-Reperfusion Injury in Rats. Turk Neurosurg 2017; 27: 952-61. [CrossRef]
  • 9. Kumaresan S, Yoganandan N, Pintar FA, Macias M, Cusick JF. Morphology of young and old cervical spine intervertebral disc tissues. Biomed Sci Instrum 2000; 36: 141-6.
  • 10. Vo N, Seo HY, Robinson A, Sowa G, Bentley D, Taylor L, et al. Accelerated aging of intervertebral discs in a mouse model of progeria. J Orthop Res 2010; 28: 1600-7. [CrossRef]
  • 11. Chan D, Song Y, Sham P, Cheung KM. Genetics of disc degeneration. Eur Spine J 2006; 15 Suppl 3: S317-25. [CrossRef]
  • 12. Adams MAR, P. J. What is intervertebral disc degeneration, and what causes it? Spine (Phila Pa 1976) 2006; 31: 2151-61. [CrossRef]
  • 13. Yuceli S. Minimally invasive surgery for one level spinal stenosis: Unilateral approach bilateral microdecompression. JTSS 2018; 29: 189-92.
  • 14. Nasto LA, Ngo K, Leme AS, Robinson AR, Dong Q, Roughley P, et al. Investigating the role of DNA damage in tobacco smoking-induced spine degeneration. Spine J 2014; 14: 416-23. [CrossRef]
  • 15. Livshits G, Popham M, Malkin I, Sambrook PN, Macgregor AJ, Spector T, et al. Lumbar disc degeneration and genetic factors are the main risk factors for low back pain in women: the UK Twin Spine Study. Ann Rheum Dis 2011; 70: 1740-5. [CrossRef]
  • 16. Sambrook PN, MacGregor AJ, Spector TD. Genetic influences on cervical and lumbar disc degeneration: a magnetic resonance imaging study in twins. Arthritis Rheum 1999; 42: 36672. [CrossRef]
  • 17. Samartzis D, Karppinen J, Chan D, Luk KD, Cheung KM. The association of lumbar intervertebral disc degeneration on magnetic resonance imaging with body mass index in overweight and obese adults: a population-based study. Arthritis Rheum 2012; 64: 148896. [CrossRef]
  • 18. Yang D, Wang D, Shimer A, Shen FH, Li X, Yang X. Glutathione protects human nucleus pulposus cells from cell apoptosis and inhibition of matrix synthesis. Connect Tissue Res 2014; 55: 132-9. [CrossRef]
  • 19. Ran Q, Liang H, Ikeno Y, Qi W, Prolla TA, Roberts LJ, 2nd, et al. Reduction in glutathione peroxidase 4 increases life span through increased sensitivity to apoptosis. J Gerontol A Biol Sci Med Sci 2007; 62: 932-42. [CrossRef]
  • 20. Ruiz FK, Bohl DD, Webb ML, Russo GS, Grauer JN. Oswestry Disability Index is a better indicator of lumbar motion than the Visual Analogue Scale. Spine J 2014; 14: 1860-5. [CrossRef]
  • 21. Vo NV, Hartman RA, Patil PR, Risbud MV, Kletsas D, Iatridis JC, et al. Molecular mechanisms of biological aging in intervertebral discs. J Orthop Res 2016; 34: 1289-306. [CrossRef]
  • 22. Urban JP, Smith S, Fairbank JC. Nutrition of the intervertebral disc. Spine (Phila Pa 1976) 2004; 29: 2700-9. [CrossRef]
  • 23. Lopez-Otin C, Blasco MA, Partridge L, Serrano M, Kroemer G. The hallmarks of aging. Cell 2013; 153: 1194-217. [CrossRef]
  • 24. Vo N, Niedernhofer LJ, Nasto LA, Jacobs L, Robbins PD, Kang J, et al. An overview of underlying causes and animal models for the study of age-related degenerative disorders of the spine and synovial joints. J Orthop Res 2013; 31: 831-7. [CrossRef]
  • 25. Dimozi A, Mavrogonatou E, Sklirou A, Kletsas D. Oxidative stress inhibits the proliferation, induces premature senescence and promotes a catabolic phenotype in human nucleus pulposus intervertebral disc cells. Eur Cell Mater 2015; 30: 89-102; discussion 3. [CrossRef]
  • 26. Katar M, Ozugurlu AF, Ozyurt H, Benli I. Evaluation of glutathione peroxidase and superoxide dismutase enzyme polymorphisms in celiac disease patients. Genet Mol Res 2014; 13: 1030-7. [CrossRef]
  • 27. Yang S, Jensen MK, Rimm EB, Willett W, Wu T. Erythrocyte superoxide dismutase, glutathione peroxidase, and catalase activities and risk of coronary heart disease in generally healthy women: a prospective study. Am J Epidemiol 2014; 180: 901-8. [CrossRef]
  • 28. Sedighi O, Makhlough A, Shokrzadeh M, Hoorshad S. Association between plasma selenium and glutathione peroxidase levels and severity of diabetic nephropathy in patients with type two diabetes mellitus. Nephrourol Mon 2014; 6(5): e21355. [CrossRef]
  • 29. Zedan H, Abdel-Motaleb AA, Kassem NM, Hafeez HA, Hussein MR. Low glutathione peroxidase activity levels in patients with vitiligo. J Cutan Med Surg 2015; 19: 144-8. [CrossRef]

Prognostic Significance of Glutathione Peroxidase (GSH-Px) Levels in Lumbar Degenerative Disc Disease

Year 2020, Volume: 10 Issue: 2, 67 - 71, 26.08.2020
https://doi.org/10.26650/experimed.2020.0011

Abstract

Objective: In this study, the main aim was to research whether decreased antioxidant activity was manifesting the intervertebral disc degeneration in a population in which the degeneration could be compatible with their ages.
Material and Method: The study group consisted of 39 patients with lumbar disc degeneration (LDD) and 37 healthy controls. Patient data including age, symptoms, neurological examination findings, lumbar MRI findings, Oswestry Disability Index (ODI) scores, and Visual Analogue Scale of pain (VAS) were used. Human Glutathione peroxidase (GSH-Px) level was determined using the Enzyme-Linked Immunosorbent Assay (ELISA) method.
Results: Serum GSH-Px levels were significantly lower in the patients with LDD when compared to the healthy controls (p=0.011).
Conclusions: In the present study, we demonstrated that, in addition to environmental factors, there is a correlation between GSHPx enzyme deficiency and lumbar disc degeneration.

References

  • 1. Miller JA, Schmatz C, Schultz AB. Lumbar disc degeneration: correlation with age, sex, and spine level in 600 autopsy specimens. Spine (Phila Pa 1976) 1988; 13: 173-8. [CrossRef] 2. Yuceli S. Facet Cysts. JTSS 2018; 29: 219-21.
  • 3. Prescher A. Anatomy and pathology of the aging spine. Eur J Radiol 1998; 27: 181-95. [CrossRef]
  • 4. Edelson JG, Nathan H. Stages in the natural history of the vertebral end-plates. Spine (Phila Pa 1976) 1988; 13: 21-6. [CrossRef]
  • 5. Maatta JH, Wadge S, MacGregor A, Karppinen J, Williams FM. ISSLS Prize Winner: Vertebral Endplate (Modic) Change is an Independent Risk Factor for Episodes of Severe and Disabling Low Back Pain. Spine (Phila Pa 1976) 2015; 40: 1187-93. [CrossRef]
  • 6. Antar V, Baran O, Yuceli S, Erdogan H, Altintas O, Baran GE, et al. Assessment of the neuroprotective effects of the acetylcholinesterase inhibitor huperzine A in an experimental spinal cord trauma model. J Neurosurg Sci 2018; 62: 128-39.
  • 7. Cecen DA, Tatarli N, Turan Suslu H, Ozdogan S, Barisik NO. Primary Dural Spinal Lymphoma Presentation of a Rare Spinal Tumor Case. Case Rep Surg 2015; 639253. [CrossRef]
  • 8. Kazanci B, Ozdogan S, Kahveci R, Gokce EC, Yigitkanli K, Gokce A, et al. Neuroprotective Effects of Pregabalin Against Spinal Cord Ischemia-Reperfusion Injury in Rats. Turk Neurosurg 2017; 27: 952-61. [CrossRef]
  • 9. Kumaresan S, Yoganandan N, Pintar FA, Macias M, Cusick JF. Morphology of young and old cervical spine intervertebral disc tissues. Biomed Sci Instrum 2000; 36: 141-6.
  • 10. Vo N, Seo HY, Robinson A, Sowa G, Bentley D, Taylor L, et al. Accelerated aging of intervertebral discs in a mouse model of progeria. J Orthop Res 2010; 28: 1600-7. [CrossRef]
  • 11. Chan D, Song Y, Sham P, Cheung KM. Genetics of disc degeneration. Eur Spine J 2006; 15 Suppl 3: S317-25. [CrossRef]
  • 12. Adams MAR, P. J. What is intervertebral disc degeneration, and what causes it? Spine (Phila Pa 1976) 2006; 31: 2151-61. [CrossRef]
  • 13. Yuceli S. Minimally invasive surgery for one level spinal stenosis: Unilateral approach bilateral microdecompression. JTSS 2018; 29: 189-92.
  • 14. Nasto LA, Ngo K, Leme AS, Robinson AR, Dong Q, Roughley P, et al. Investigating the role of DNA damage in tobacco smoking-induced spine degeneration. Spine J 2014; 14: 416-23. [CrossRef]
  • 15. Livshits G, Popham M, Malkin I, Sambrook PN, Macgregor AJ, Spector T, et al. Lumbar disc degeneration and genetic factors are the main risk factors for low back pain in women: the UK Twin Spine Study. Ann Rheum Dis 2011; 70: 1740-5. [CrossRef]
  • 16. Sambrook PN, MacGregor AJ, Spector TD. Genetic influences on cervical and lumbar disc degeneration: a magnetic resonance imaging study in twins. Arthritis Rheum 1999; 42: 36672. [CrossRef]
  • 17. Samartzis D, Karppinen J, Chan D, Luk KD, Cheung KM. The association of lumbar intervertebral disc degeneration on magnetic resonance imaging with body mass index in overweight and obese adults: a population-based study. Arthritis Rheum 2012; 64: 148896. [CrossRef]
  • 18. Yang D, Wang D, Shimer A, Shen FH, Li X, Yang X. Glutathione protects human nucleus pulposus cells from cell apoptosis and inhibition of matrix synthesis. Connect Tissue Res 2014; 55: 132-9. [CrossRef]
  • 19. Ran Q, Liang H, Ikeno Y, Qi W, Prolla TA, Roberts LJ, 2nd, et al. Reduction in glutathione peroxidase 4 increases life span through increased sensitivity to apoptosis. J Gerontol A Biol Sci Med Sci 2007; 62: 932-42. [CrossRef]
  • 20. Ruiz FK, Bohl DD, Webb ML, Russo GS, Grauer JN. Oswestry Disability Index is a better indicator of lumbar motion than the Visual Analogue Scale. Spine J 2014; 14: 1860-5. [CrossRef]
  • 21. Vo NV, Hartman RA, Patil PR, Risbud MV, Kletsas D, Iatridis JC, et al. Molecular mechanisms of biological aging in intervertebral discs. J Orthop Res 2016; 34: 1289-306. [CrossRef]
  • 22. Urban JP, Smith S, Fairbank JC. Nutrition of the intervertebral disc. Spine (Phila Pa 1976) 2004; 29: 2700-9. [CrossRef]
  • 23. Lopez-Otin C, Blasco MA, Partridge L, Serrano M, Kroemer G. The hallmarks of aging. Cell 2013; 153: 1194-217. [CrossRef]
  • 24. Vo N, Niedernhofer LJ, Nasto LA, Jacobs L, Robbins PD, Kang J, et al. An overview of underlying causes and animal models for the study of age-related degenerative disorders of the spine and synovial joints. J Orthop Res 2013; 31: 831-7. [CrossRef]
  • 25. Dimozi A, Mavrogonatou E, Sklirou A, Kletsas D. Oxidative stress inhibits the proliferation, induces premature senescence and promotes a catabolic phenotype in human nucleus pulposus intervertebral disc cells. Eur Cell Mater 2015; 30: 89-102; discussion 3. [CrossRef]
  • 26. Katar M, Ozugurlu AF, Ozyurt H, Benli I. Evaluation of glutathione peroxidase and superoxide dismutase enzyme polymorphisms in celiac disease patients. Genet Mol Res 2014; 13: 1030-7. [CrossRef]
  • 27. Yang S, Jensen MK, Rimm EB, Willett W, Wu T. Erythrocyte superoxide dismutase, glutathione peroxidase, and catalase activities and risk of coronary heart disease in generally healthy women: a prospective study. Am J Epidemiol 2014; 180: 901-8. [CrossRef]
  • 28. Sedighi O, Makhlough A, Shokrzadeh M, Hoorshad S. Association between plasma selenium and glutathione peroxidase levels and severity of diabetic nephropathy in patients with type two diabetes mellitus. Nephrourol Mon 2014; 6(5): e21355. [CrossRef]
  • 29. Zedan H, Abdel-Motaleb AA, Kassem NM, Hafeez HA, Hussein MR. Low glutathione peroxidase activity levels in patients with vitiligo. J Cutan Med Surg 2015; 19: 144-8. [CrossRef]
There are 28 citations in total.

Details

Primary Language English
Subjects Clinical Sciences
Journal Section Research Article
Authors

Cumhur Kaan Yaltırık This is me 0000-0002-4312-5685

Seda Güleç Yılmaz This is me 0000-0002-8119-2862

Fatma Tuba Akdeniz This is me 0000-0002-6076-0509

Kadir Sümerkent This is me 0000-0001-6942-4491

Selçuk Özdoğan This is me 0000-0003-1711-5771

Turgay İşbir This is me 0000-0002-7350-6032

Publication Date August 26, 2020
Submission Date June 3, 2020
Published in Issue Year 2020 Volume: 10 Issue: 2

Cite

Vancouver Yaltırık CK, Güleç Yılmaz S, Akdeniz FT, Sümerkent K, Özdoğan S, İşbir T. Prognostic Significance of Glutathione Peroxidase (GSH-Px) Levels in Lumbar Degenerative Disc Disease. Experimed. 2020;10(2):67-71.