Investigation of Galectin-3 Levels of Endometriosis Patients According to Stages

Year 2023, Volume: 13 Issue: 1, 15 - 20, 11.05.2023

D. Fulya Kızılgedik Armagan Caner Çağlar Yıldız Buğra Okşaşoğlu Sema Mısır İlhan Yaylım Semra Demokan Ceylan Hepokur

https://doi.org/10.26650/experimed.1148905

Abstract

Objective: Endometriosis is a gynecological disease associated with chronic pelvic inflammation, pain, and infertility. Galectin-3 (Gal-3) is a protein that can bind to β-galactosides, which plays an important role in different biological processes according to the stages of the disease in patients with endometriosis. This study aimed to elucidate the importance of Gal-3 in endometriosis, to reveal its potential power as a non-invasive diagnostic biomarker in disease etiopathogenesis.
Materials and Methods: The serum concentration of Gal-3 and cancer antigen 125 (CA-125) from whole blood were measured using enzyme-linked immuno sorbent assay (ELISA) and an auto-analyzer, respectively. Gal-3 expression was determined by quantitative real-time polymerase chain reaction (qRT-PCR) from peripheral blood.
Results: We found significant differences for Gal-3 expression levels between the endometriosis and control groups (p <0.05). Gal-3 levels in the serum of women with endometriosis are also remarkably increased compared with the control group.
Conclusion: Galectin-3 can play critical functions in the development and progression of endometriosis, so, further studies are needed in this area

Keywords

Galectin-3, endometriosis, biomarkers, qRT-PCR

Project Number

Ecz-075

References

• 1. Bulun SE. Mechanisms of disease endometriosis. N Engl J Med 2009; 360: 268-79. [CrossRef] google scholar
• 2. Pabalan N, Jarjanazi H, Christofolini DM, Barbosa CP, Bianco B. Association of the intercellular adhesion molecule-1 (ICAM-1) gene polymorphisms with endometriosis: A systematic review and metaanalysis. Arch Gynecol Obstet 2015; 292: 843-51. [CrossRef] google scholar
• 3. Meggyes M, Szereday L, Bohonyi N, Koppan M, Szegedi S, Marics-Kutas A et al. Different expression pattern of TIM-3 and Galectin-9 molecules by peripheral and peritoneal lymphocytes in women with and without endometriosis. Int J Mol Sci 2020; 21(7): 2343. [CrossRef] google scholar
• 4. Aghajanpour L, Mashayekhi F, Rajaei F. Intercellular adhesion molecule-1 (ICAM-1) gene polymorphism and endometriosis in northern Iran. Arch Gynecol Obstet 2011; 283:1035-9. [CrossRef] google scholar
• 5. Caserta D, Di Benedetto L, Bordi G, D’Ambrosio A, Moscarini M. Levels of Galectin-3 and stimulation expressed gene 2 in the peritoneal fluid of women with endometriosis: A pilot study. Gynecol Endocrinol 2014; 30(12): 877-80. [CrossRef] google scholar
• 6. Sharpe Timms KL. Endometrial anomalies in women with endometriosis. Ann NY Acad Sci 2001; 943: 131-47. [CrossRef] google scholar
• 7. Bastón JI, Barañao RI, Ricci AG, Bilotas MA, Olivares CN, Singla JJ. Targeting galectin-1-induced angiogenesis mitigates the severity of endometriosis. J Pathol 2014; 234(3): 329-37. [CrossRef] google scholar
• 8. Crosignani P, Olive D, Bergqvist A, Luciano A. Advances in the management of endometriosis: An update for clinicians. Hum Reprod Update 2006; 12: 179-89. [CrossRef] google scholar
• 9. Heidemann LN, Hartwell D, Heidemann CH, Jochumsen KM. The relation between endometriosis and ovarian cancer - a review. Acta Obstet Gynecol Scand 2014; 93(1): 20. [CrossRef] google scholar
• 10. Cho S, Mutlu L, Grechukhina O, Taylor HS. Circulating microRNAs as potential biomarkers for endometriosis. Fertil Steril 2015; 103(5): 1252-60. [CrossRef] google scholar
• 11. Van den Brûle FA, Fernandez PL, Buicu C, Liu FT, Jackers P, Lambotte R, et al. Differential expression of galectin-1 and galectin-3 during first trimester human embryogenesis. Dev Dyn 1997; 209: 399405. [CrossRef] google scholar
• 12. Fowlis D, Colnot C, Ripoche MA, Poirier F. Galectin-3 is expressed in the notochord, developing bones, and skin of the post implantation mouse embryo. Dev Dyn 1995; 203(2): 241-51. [CrossRef] google scholar
• 13. Kyama CM, Debrock S, Mwenda JM, D’Hooghe TM. Potential involvement of the immune system in the development of endometriosis. Reprod Biol Endocrinol 2003; 1: 123. [CrossRef] google scholar
• 14. Brubel R, Bokor A, Pohl A, Schilli GK, Szereday L, Bacher-Szamuel R et al. Serum galectin-9 as a noninvasive biomarker for the detection of endometriosis and pelvic pain or infertility-related gynecologic disorders. Fertil Steril 2017; 108(6): 1016-25. [CrossRef] google scholar
• 15. F O Dorien, Flores Idhaliz, Waelkens E, D’Hooghe T. Noninvasive diagnosis of endometriosis: Review of current peripheral blood and endometrial biomarkers. Best Pract Res Clin Obstet Gynaecol 2018; 50: 72-83. [CrossRef] google scholar
• 16. Fassbender A, Burney RO, O DF, D’ Hooghe T, Giudice L. Update on biomarkers for the detection of endometriosis. BioMed Res Int 2015; 2015: 130854. [CrossRef] google scholar
• 17. May KE, Conduit-Hulbert SA, Villar J, Kirtley S, Kennedy SH, Becker CM. Peripheral biomarkers of endometriosis: A systematic review. Hum Reprod Update 2010; 16: 651-74. [CrossRef] google scholar
• 18. Noël JC, Chapron C, Borghese B, Fayt I, Anaf V. Galectin-3 is overexpressed in various forms of endometriosis. Appl. Immunohistochem Mol Morphol. 2011; 19: 253-7. [CrossRef] google scholar
• 19. Vergetaki A, Jeschke U, Vrekoussis T, Taliouri E, Sabatini L, Papakonstanti EA, et al. Galectin-1 overexpression in endometriosis and its regulation by neuropeptides (CRH, UCN) indicating its important role in reproduction and inflammation. PLoS One 2014; 9: e114229. [CrossRef] google scholar
• 20. Brinchmann MF, Patel DM, Iversen MH. The role of Galectins as modulators of metabolism and inflammation. Mediat Inflamm 2018: 9186940. [CrossRef] google scholar
• 21. Mattos RM, Machado DE, Perini JA, Alessandra-Perini J, Costa Nathália de OM, Oliveira Wiecikowsk AFR, et al. Galectin-3 plays an important role in endometriosis development and is a target to endometriosis treatment. Mol Cell Endocrinol 2019; 486: 1-10. [CrossRef] google scholar
• 22. Dong R, Zhang M, Hu Q, Zheng S, Soh A, Zheng Y, Yuan H. Galectin-3 as a novel biomarker for disease diagnosis and a target for therapy (Review). Int J Mol Med 2018; 41(2): 599-614. [CrossRef] google scholar
• 23. Hisrich BV, Young RB, Sansone AM, Bowens Z, Green LJ, Lessey Bruce A, et al. Role of human galectins in inflammation and cancers associated with endometriosis. Biomolecules 2020; 10(2): 230. [CrossRef] google scholar
• 24. Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta C(T)) method. Methods 2001; 25(4): 402-8. [CrossRef] google scholar
• 25. Rekker K, Saare M, Roost AM, Kaart T, Söritsa De, Karro H, et al. Circulating miR-200-family micro-RNAs have altered plasma levels in patients with endometriosis and vary with blood collection time. Fertil Steril 2015; 104(4): 938-46. [CrossRef] google scholar
• 26. Hsu AL, Khachikyan I, Stratton P. Invasive and non-invasive methods for the diagnosis of endometriosis. Clin Obstet Gynecol 2010; 53(2): 413-9. [CrossRef] google scholar
• 27. Meeusen JW, Johnson JN, Gray A, Wendt P, Jefferies JL, Jaffe AS, et al. Soluble ST2 and galectin-3 soluble ST2 and galectin-3 in pediatric patients without heart failure. Clin Biochem 2015; 48: 1337-40. [CrossRef] google scholar
• 28. Schindler EI, Szymanski JJ, Hock KG, Geltman EM, Scott MG: Short-and long-term biologic variability of galectin-3 and other cardiac biomarkers in patients with stable heart failure and healthy adults. Clin Chem 2016; 62: 360-6. [CrossRef] google scholar
• 29. Johannes L, Jacob R Leer H. Galectins at a glance. J Cell Sci 2018; 131. [CrossRef] google scholar
• 30. Von Wolff M, Wang X, Gabius HJ, Strowitzki T. Galectin fingerprinting in human endometrium and decidua during the menstrual cycle and in early gestation. Mol Hum Reprod 2005; 11: 189-94. [CrossRef] google scholar
• 31. Yang H, Yin J, Ficarrotta K, Hsu SH, Zhang W, Cheng C. Aberrant expression and hormonal regulation of Galectin-3 in endometriosis women with infertility. J Endocrinol Invest 2016; 39(7): 785-91. [CrossRef] google scholar
• 32. De Mattos RM, Pereira PR, de Oliveira Barros EG, da Silva JH, Palmero CY, da Costa NM, et al. Aberrant levels of Wnt/ß-catenin pathway components in a rat model of endometriosis. Histol Histopathol 2016; 31: 933-42. google scholar