Gene Expression Profile as a Precursor of Inflammation in Mouse Models: BFMI860 and C57BL/6NCrl

Year 2024, Volume: 14 Issue: 2, 73 - 84, 26.08.2024

Ayça Doğan , Gudrun A. Brockmann

https://doi.org/10.26650/experimed.1384602

Abstract

Objective: We aimed to investigate the differences in the immune response to body fat content between the genetically mutant obese BFMI860 (BFMI) mouse strain and the lean C57BL/6Ncrl (B6) mouse strain as a control and the effects of obesity on gene expression on inflammation-related pathways in epididymal adipose tissue.
Materials and Methods: Six males from each strain were maintained on a standard maintenance diet (SMD) or a high-fat diet (HFD). At the age of 10 weeks, serum and epididymal adipose tissue samples were collected for cytokine and gene expression analyses. RNA samples from epididymal adipose tissue were hybridized using the microarray technique to study the quantitative transcript amounts of genes.
Results: Pathway analysis of gene expression data revealed no considerable development of inflammatory state in BFMI and B6 on SMD. Both strains responded to HFD distinctly; the inflammatory state was more prominent in the obese BFMI group than in the lean B6 group. Several genes, such as Adipoq, NFkbia, Plaur, F2r, C3ar1, and Nfatc4 in pathways involved in the immune system have been found to be differentially regulated in BFMI mice. Under the condition of obesity in BFMI mice, the induction of inflammation-related pathways indicates an increased risk of insulin resistance, atherosclerosis, and cardiovascular disease.
Conclusion: This study identified distinct expression patterns of genes involved in inflammatory pathways, particularly those associated with the adipocytokine signaling pathway and complement and coagulation cascades, in the epididymal adipose tissue of BFMI and B6 mice. The BFMI strain is a valuable and promising model for clarifying the mechanisms underlying obesity and the activation of inflammation in adipose tissue.

Keywords

Obesity, high-fat diet, adipocytokine signaling pathway, complement and coagulation cascade

Ethical Statement

All experimental procedures were approved by the German Animal Welfare Authorities (approval no. G0152/04).

Supporting Institution

erman National Genome Research Network, German Research Foundation ,German Network for Systems Genetics

Thanks

This research was supported by grants of the German National Genome Research Network (NGFN: 01GS0486, 01GS0829) by grants of the German Research Foundation (GRK1209) and German Network for Systems Genetics (GeNeSys).

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