The Potency of Beligo Seeds (Benincasa hispida (Thunb.) Cogn.) as Antihyperlipidemic in L-NAME-induced Hyperlipidemic Rats

Year 2023, , 231 - 240, 01.08.2023

Nur Alım

https://doi.org/10.55262/fabadeczacilik.1200880

Abstract

Beligo or Bligo is the name of Benincasa hispida (Thunb.) Cogn. in Indonesian, is empirically used in the treatment of cholesterol and hypertension. The part of the plant used is the seed. This have a look at aimed to determine the antihyperlipidemic activity of beligo seeds in L-NAME-induced hyperlipidemic rats. Male albino rats (n= 25) were divided into 5 groups: Sod. CMC 1%, plant extract (100 mg/kg, 200 mg/kg, 300 mg/kg body weight), and Simvastatin were induced by L-NAME 40 mg/kg BW for 4 weeks. Measurement of lipid profile levels consisting of TC, HDL, LDL, TG, and VLDL, using a human analyzer (Thermo Scientific Indico®). L-NAME was shown to significantly (p<0.05) improve lipid profile levels. The results showed that beligo seed ethanol extract (BSEE) at a dose of 200 mg/kg BW, 300 mg/kg BW can reduce significantly (p<0,05) levels of TC, TG, and LDL and can increase significantly (p<0,05) HDL levels. While a dose of 100 mg/kg BW can only reduce significantly (p<0,05) TC and TG levels and only a dose of 300 mg/kg BW can reduce VLDL significantly (p<0,05).

Keywords

Benincasa hispida (Thunb.) Cogn. Seed, Hyperlipidemic, L-NAME, Lipid profile

Supporting Institution

makassar islamic university and hasanuddin university

Project Number

3

Thanks

thanks to all advisor

References

• Alim, N., Hasan, T., Rusman, Jasmiadi, & Zulfitri. (2022). Phytochemical Screening , Relationship of Total Phenolic with Antioxidant Activity Of Ethanol and Methanol Extracts of Kesambi ( Schleichera oleosa ( Lour .) Oken ) Bark Skrining Fitokimia dan Hubungan Kadar Fenolik Total dengan Aktivitas Antioksidan Ekst. 22(2), 118–124.
• Alim, N., Jummah, N., Pratama, A. S., & Nurdiyanti, N. (2021). Skirining fitokimia ekstrak etanol kulit buah sirsak (Annona muricata Linn) dan uji aktivitas antioksidan dengan metode DPPH. Sasambo Journal of Pharmacy. https://doi.org/10.29303/sjp.v2i2.40
• Al-snafi, A. E. (2013). The Pharmacological Importance of Benincasa hispida . A review. 4(12), 165–170.
• Aqilah, N., Hamid, A. A., Aided, C., & Design, D. (2010). Nutrıtıonal Composıtıon and Oıl Fatty Acıds of Kundur [Benincasa hispida (Thunb.) Cogn.] Seed. Pakistan Journal of Botany, 42(5)(March 2014), 3247–3255.
• Bhattarai, A. K., Acharya, A., & Karki, P. K. (2020). Use of Statins as Lipid Lowering Agent in Hypercholesterolemia in a Tertiary Care Hospital : A Descriptive Cross-sectional Study. J Nepal Med Assoc, 58(232), 1031–1035. https://doi.org/10.31729/jnma.5444
• Burdge, G. C., & Calder, P. C. (2015). Introduction to fatty acids and lipids. World review of nutrition and dietetics, 112, 1–16. https://doi.org/10.1159/0003654
• Harris, C. P., Berg, A. Von, Berdel, D., Bauer, C., Schikowski, T., Koletzko, S., … Standl, M. (2018). Association of Dietary Fatty Acids with Blood Lipids is Modified by Physical Activity in Adolescents : Results from the GINIplus and LISA Birth Cohort Studies. https://doi.org/10.3390/nu10101372
• Kontostathi, M., Isou, S., Mostratos, D., Vasdekis, V., Demertzis, N., Kourounakis, A., … Black, H. S. (2021). Influence of Omega-3 Fatty Acid-Rich Fish Oils on Hyperlipidemia: Effect of Eel, Sardine, Trout, and Cod Oils on Hyperlipidemic Mice. 24(7), 749–755. https://doi.org/10.1089/jmf.2020.0114
• Ramchoun, M., Khouya, T., Harnafi, H., Alem, C., Benlyas, M., Simmet, T., … Amrani, S. (2020). Effect of polyphenol , flavonoid , and saponin fractions from Thymus atlanticus on acute and chronic hyperlipidemia in mice. Future Journal of Pharmaceutical Sciences, 6:69. https://doi.org/10.1186/s43094-020-00097-z
• Samad, N. B., Debnath, T., Jin, H. L., Lee, B. R., Park, P. J., Lee, S. Y., & Lim, B. O. (2013). Antioxidant activity of Benincasa hispida seeds. Journal of Food Biochemistry, 37(4), 388–395. https://doi.org/10.1111/j.1745-4514.2011.00643.x
• Shaul PW. Endothelial nitric oxide synthase, caveolae and the development of atherosclerosis. J Physiol. 2003;547(1):21–33 Shu X, Keller TS, Begandt D, Butcher JT, Biwer L, Keller AS, et al. (2015). Endothelial nitric oxide synthase in the microcirculation. Cell Mol Life Sci. 72(23):4561–75.
• Surya, S., Arun Kumar, R., Carla, B., Sunil, C. 2017. Antihyperlipidemic effect of Ficus dalhousiae miq. Stem bark on Triton WR-1339 and high fat diet-induced hyperlipidemic rats. Bull. Fac. Pharm. Cairo. Univ
• Tata, C. M., Sewani-Rusike, C. R., Oyedeji, O. O., Gwebu, E. T., Mahlakata, F., & Nkeh-Chungag, B. N. (2019). Antihypertensive effects of the hydro-ethanol extract of Senecio serratuloides DC in rats. BMC Complementary and Alternative Medicine, 19(1), 1–10. https://doi.org/10.1186/s12906-019-2463-2
• Talas ZS, Ozdemir I, Ciftci O, Cakir O, Gulhan MF, Pasaoglu OM. (2014). Role of propolis on biochemical parameters in kidney and heart tissues against L-NAME induced oxidative injury in rats. Clin Exp Hypertens, 36(7):492–6.
• Vuilleumier N, Bratt J, Alizadeh R, Jogestrand T, Hafström I, Frostegård J. 2010. Anti-apoA-1 IgG and oxidized LDL are raised in rheumatoid arthritis (RA):potential associations with cardiovascular disease and RA disease activity. Scand J Rheumatol. 39(6):447–53.
• Welty, F. K., Lewis, S. J., Friday, K. E., Cain, V. A., & Anzalone, D. A. (2016). A Comparison of Statin Therapies in Hypercholesterolemia in Women : J Womens Health (Larchmt), 25(1), 50–56. https://doi.org/10.1089/jwh.2015.5271