Immunosuppressive and Ameliorative Effects of Dietary Combined Herbs Extract of Curcuma zedoaria (Christm.) Roscoe and Phyllanthus niruri L. in DMBA-induced Breast Cancer Mouse Model

Year 2024, , 91 - 110, 26.03.2024

Wira Eka Putra , Anoraga Mona Larasati Sekar Mentarı Devita Ratnasarı Dhaniar Chaırunnıza Arief Hidayatullah , Muhaimin Rifa`i

https://doi.org/10.55262/fabadeczacilik.1329094

Abstract

Cancer still become the top health problem globally. Each year, there is an increased number of new incidence and mortality. A ton of modalities have been proposed as therapeutic strategy, including the application of medicinal plant-based therapy. In this study, we aimed to evaluate the immunomodulation effect of dietary combined herbs extract of Curcuma zedoaria and Phyllanthus niruri (cheral) in DMBA-induced breast cancer mouse model. About six groups of treatment were applied in experimental mice, including the administration of cheral extract with series of dose including 1.233 mg/kg BW, 2.466 mg/kg BW, and 4.932 mg/kg BW. Flow cytometry analysis was performed to evaluate immune parameters including NK cells, TNF-α and IFN-γ-expressing NK cells, CD4+TNF-α+IFN-γ+ type 1 helper T cells, and CD4+CD25+ regulatory T cells population. In this present study, we demonstrated that cheral extract exerts the immunosuppressive activity by attenuate the properties of immune system, such as NK cells, Th1 cells, and regulatory T cells in DMBA-induces mouse into normal level. Thus, we suggested that cheral extract has ameliorative potency as alternative or complementary therapy against cancer incidence.

Keywords

Cheral, Curcuma zedoaria, DMBA, Immunosuppressive, Phyllanthus niruri

References

• Tjandrawinata R, Susanto L & Nofiarny D. (2017). The use of Phyllanthus niruri L. as an immunomodulator for the treatment of infectious diseases in clinical settings. Asian Pacific Journal of Tropical Disease 7: 132–140