EGCg Supplementation Improves Oxidant and Antioxidant Status in Kidney of Rats Exposed to Sevoflurane

Abstract

Objectives: To investigate the possible effects of sevoflurane anesthesia and epigallocatechin 3- gallate (EGCg) on kidney tissue by evaluating the oxidant and antioxidant status in rats. Materials and Methods: Tree groups of animals were studied. Sevoflurane 3% (v/v) in air/O2 were administered to animals in group 1 (n = 7) and sevoflurane plus EGCg was administered in group 2 (n = 7). Seven animals were allocated to control group (group 3). Malondialdehyde (MDA), nitric oxide (NO), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT) in kidney tissue were studied. Histopathological changes were also assessed. Results: In group I, MDA, NO, SOD and GSH-Px activities were increased while decreased CAT activities occurred when sevoflurane was exposured compared with controls. Supplementation of EGCg (group 2) decreased the MDA (P

Keywords

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EGCg İlavesi Sevofluran Uygulanan Ratların Böbreklerinde Oksidan ve Antioksidan Durumunu Düzenler

Öz

Amaç: Ratlarda böbrek dokusu üzerinde oksidan ve antioksidan durumu değerlendirerek sevofluran anestezisi ve epigallocatechin 3- gallate (EGCg)'ın muhtemel etkilerini araştırmayı amaçladık. Gereç ve Yöntem: Ratlar 3 gruba ayrılarak çalışıldı. Grup 1 (n:7): Sevofluran, %3 (v/v) hava/O2 karışımı ile uygulandı. Grup 2 (n:7): Sevofluran + EGCg uygulandı. Kontrol grubu için ise yine 7 tane rat seçildi. Malondialdehid (MDA), nitrik oksit (NO), süperoksit dismutaz (SOD), glutatyon peroksidaz (GSH-Px) ve katalaz (CAT) böbrek dokusunda çalışıldı. Ayrıca histopatolojik değişiklikler değerlendirildi. Bulgular: Grup 1'de MDA, NO, SOD ve GSH-Px aktiviteleri artmışken, CAT aktivitesi sevofluran uygulananlarda azalmıştır. EGCg'nin uygulaması (grup 2) MDA, SOD ve GSH-Px aktivitelerini azaltırken, NO ve CAT aktiviteleri grup 1 ile karşılaştırıldığında artmış olarak bulunmuştur. Histopatolojide sevofluran uygulanan grupta böbrek dokusunda orta derecede kortikal nekroz ve şiddetli dejenerasyonla karakterize değişimlerin oluştuğu saptandı. Sevofluran ile birlikte EGCg uygulamasının ise dejenerasyonu azalttığı bulunmuştur. Sonuç: Lipid peroksidasyonu ve antioksidan enzim düzeyleri sevofluran uygulamasını takiben fazla miktarda artmıştır. Ancak, EGCg uygulaması böbrek dokusunu önemli ölçüde korumaktadır. ©2008, Fırat Üniversitesi, Tıp Fakültesi

Anahtar Kelimeler

• EGCg, Sevofluran, oksidan-antioksidan sistem

References

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25. Kabul Tarihi: 10.08.2007