Effect of Inhaled N-Acetyl-L-Cysteine Treatment on Induced Sputum Glutathione and Nitrite

Abstract

Objective: An imbalance between oxidants-antioxidants is known to play an important role in the pathogenesis of asthma, especially during exacerbation. The aim of this study was to investigate the effect of N-acetyl-L-cysteine (NAC) treatment on the levels of sputum nitrit (NO2-) and reduced glutathione (GSH) contents in patients with asthma during exacerbations. Materials and Methods: The study had a double blind, placebo controlled design. Induced sputum GSH, NO2 -, cell counts and plasma NO2 – contents were evaluated in 11 healthy controls (HCs) and 25 patients with asthma during exacerbation before and after treatment. Fifteen patients with acute asthma attack were treated with inhaled NAC during exacerbation. Results: Plasma-sputum NO2 - and sputum GSH contents were significantly higher in subjects with asthma subgroups than HCs (p

Keywords

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Astım Atağındaki Hastalarda İnhaler N-Asetil-L-Sistein Tedavisinin İndükte Balgamda Glutatyon ve Nitrit Seviyeleri Üzerine Etkisi

Öz

Amaç: Oksidanlar ile antioksidanlar arasındaki dengesizlik astım patogenezinde, özellikle de ataklar sırasında önemli rol oynamaktadır. Bu çalısmanın amacı N-asetil-L-sistein (NAC) tedavisinin astım atağındaki hastaların balgam nitrit (NO2 -) ve redükte glutatyon (GSH) seviyeleri üzerine etkilerini araştırmaktır. Gereç ve Yöntem: Bu çift-kör plasebo kontrollü bir çalışmadır. Onbir sağlıklı kontrol (SK) ve 25 astım atağındaki hastanın indükte balgamlarında GSH, NO2 - seviyeleri, hücre sayımları ve plazma NO2 - seviyeleri tedavi öncesi ve sonrası dönemde değerlendirildi. Akut astım atağındaki 15 hastaya atak süresince inhaler NAC tedavisi verildi. Bulgular: Astım subgruplarında plazma ve balgam NO2 - ve balgam GSH seviyeleri SK grubunda anlamlı derecede yüksek bulundu (plazma NO2 - seviyesi için; NAC tedavisi alan grupta p

Anahtar Kelimeler

• İndükte balgam, glutatyon, nitrit, astım atağı, N-asetil-L-sistein

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