Relationship between the intensity of helicobakter pylori and number of mast cells in cases of the antral gastritis

Abstract

Objectives: Helicobacter pylori (HP) has an important role in various gastroduodenal diseases, especially in active and chronic gastritis and mast cells is known as primary trigger of inflammation. Due to that, the relationship between the degree of HP positivity and negativity with the intensity of mast cell in the cases of antral gastritis was invested. Materials and Methods: 160 cases that were reported as antral gastritis by Fırat Ünivercity Medicine Faculty Pathology Laboratory but not diagnosed as duodenal ulcer via endoscopic examination previously, were included in this study. The study group was divided in to 4 subgroup as HP(-), HP 1+, HP 2+ and HP 3+ and each group has 40 cases. In this study, the count of mast cell in these subgroups was compared. Results: Mast cell count in antral gastritis in HP(-), HP 1+, HP 2+, HP 3+ cases was found (63,92±10,02, 86,27±11,08, 92,75±13,59, 121±17,73) respectively. The difference between HP(-) with HP 1+ cases and HP 2+ with HP 3+ cases about mast cell count was significant (p

Keywords

• -

Antral Gastritlerde Helikobakter Pilori Yoğunluğu ve Mast Hücre Sayısı Arasındaki İlişki

Abstract

Amaç: Helikobakter Pilori (HP), aktif ve kronik gastritler başta olmak üzere çeşitli gastroduodenal hastalıkların etiyolojisinde önemli rol oynar. Mast hücrelerinin de inflamasyonun primer tetikleyicisi olduğu bilinmektedir. Bu nedenle antral gastritli olgularda HP negatifliği ve HP pozitiflik derecesi ile mast hücre yoğunluğu arasındaki ilişki araştırıldı. Gereç ve Yöntem: Fırat Üniversitesi Tıp Fakültesi Patoloji Anabilim Dalı Laboratuarında antral gastrit tanısı alan ve endoskopik muayenesinde duodenal ülser bulunmayan 160 olgu çalışmaya alındı. Her birinden 40 olmak üzere HP(-), HP1+, HP 2+ ve HP 3+ antral gastritli olgularda mast hücre sayıları karşılaştırıldı. Bulgular: Mast hücre sayısı antral gastritlerde HP(-) olgularda 63.92±10.02, HP 1+ olgularda 86.27±11.0.8. HP 2+ olgularda 92.75±13.59. HP 3+ olgularda 121±17.73 bulundu. HP(-) olgularla HP1+ ve HP 2+ olgularla HP 3+ olgular arasında mast hücre sayısı arasında istatistiksel olarak anlamlı farklılık (p0,05). Sonuç: İltihabi proçeste önemli rol oynayan mast hücrelerinin yoğunluğu, HP pozitifliği ile belirgin olarak artmaktadır. Özellikle HP(-) ve HP1+ olgular arasında mast hücre sayısında anlamlı bir farklılık ve HP pozitiflik derecesi ile mast hücre sayılarında belirgin artış bulunması, antral gastritlerde HP pozitiflik derecesiyle mast hücre artışının ilişkili olduğunu düşündürmektedir. ©2006, Fırat Üniversitesi, Tıp Fakültesi

Keywords

• Helikobakter pilori, antral gastrit, mast hücresi

References

1. Theoharides TC, Cochrane DE. Critical role of mast cells in inflammatory diseases and the effect of acute stres. Journal of Neuroimmunology 2004; 146: 1-12.
2. Sulik A, Kemona A, Sulik M. The gastrointestinal mast cell in health and disease. Rocz Akad Med Bialymst. 1999; 44: 17-23
3. Nakajima S, Krıshnan B, Ota H et al. Mast cell involvement in gastritis with or withouth helicobacter pylori infection. Gastroenterology 1997; 113: 746-754
4. Warran JR,Marshall B. Unidentified curved bacili on gastric epithelium in active chronic gastritis. Lancet 1983; 1: 1273-1275
5. Dixon MF, Genta RM, Yardley JH, Correa P. Classification and grading of gastritis. The updated Sydney System. International Workshop on the Histopathology of Gastritis, Houston 1994. Am J Surg Pathol 1996; 20: 1161-1181
6. Genta RM. Helicobacter pylori infection in gastric pathology. In: Weinstein RS, ed. Advances in pathology and laboratory medicine. Volume 7. St. Louis: Mosby-Year Book, 1994: 443465
7. Enerback L. Mucosal mast cells in the rat and in man. Int Arch Allergy Appl Immunol 1987; 82: 249-255.
8. Serafin WE, Austen KF. Mediators of immediate hipersensivity reactions. N Engl J Med 1987; 317: 30-34.

9. Marshall JS, Bienenstock J. The role of mast cells in inflammatory reactions of the airways, skin and intestine. Curr Opin Immunol 1994; 6: 853-859
10. Nakajima S, Graham DY, Hattori T, Bamba T. Strategy for treatment of Helicobacter pylori infection in adults. I. Updated indications for test and eradication therapy suggested in 2000. Curr Pharm Des 2000; 6: 1503-1514.
11. Walls AF, He S, Teran LM et al. Granulocyte recruitment by human mast cell tryptase. Int Arch Allergy Immunol 1995; 107: 372-373
12. Goetzl EJ, Derian CK, Tauber AI, Valone FH . Novel effects of 1-0-hexadecyl-2-acyl-sn-glycero-3-phosphorylcholine mediators on human leucocyte function: delineation of the specific roles of the acyl substituents. Biochem Biophys Res Commun 1980; 94: 881-888
13. Stachura J, Konturek JW, Dembinski A, Domschke W. Do infiltrating leucocytes contribute to the adaptation of human gastric mucosa to continued aspirin administration? Scand J Gastroenterol 1994; 29: 966-72
14. Aktepe F, Irkkan Ç, Kapucuoğlu N, Pak I. Helicobacter pylori gastriti ile mast hücre yoğunluğu arasındaki ilişki. XIV. Ulusal Patoloji Kongresi Bildiri Özetleri Kitabı 1999; D-14:273
15. Müezzinoğlu B, Gürbüz Y, Şentürk Ö. Mast cells in antral gastritis: Associated Helicobacter pylori and duodenal ulcer. Patoloji Bülteni 2001; 18: 24-26
16. Galli SJ. New concepts about the mast cell. New Engl J Med 1993; 328: 257-265
17. Schwartz LB. Mast cell: function and contents. Curr Opin Immunol 1994; 6: 91-97.
18. Gordon JR, Burd PR, Gali SJ. Mast cells as source of multifunctional cytokines. Immunol Today 1990; 11: 458-464
19. Noach LA, Bosma NB, Jansen J et al. Mucosal tumor necrosis factor α, Interleukin 1β, Interleukin 8 production in patients with Helicobacter pylori infection. Scand J Gastroenterol 1994; 29: 425-29
20. Bamba N, Nakajima S, Andoh A et al. Stem cell factor expressed in human gastric mucosa in relation to mast cell increase in Helicobacter pylori-infected gastritis. Dig Dis Sci 2002; 47: 274282
21. Crabtree JE, Wyatt JI, Trejdosiewicz LK et al. Interleukin 8 expression in Helicobacter pylori, normal and neoplastic gastroduodenal mucosa. J. Clin Pathol 1994;47:61-66
22. Gionchetti P, Vaira D, Campieri M, et al. Enhanced mucosal Interleukin 6 and 8 in Helicobacter pylori-positive dyspeptic patients. Am J Gastroenterol 1994;89:883-887
23. Walls AF, Jones DB, Williams JH, Church MK, Holgate ST. Immunohistochemical identification of mast cell in formaldehydfixed tissue using monoclonal antibodies specific for tryptase. J Pathol 1990; 162: 119-126.