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The Effects of Vasodilator Solutions on Apoptosis in Saphenous Vein with Mechanical Distention Application

Year 2012, Volume: 17 Issue: 2, 94 - 99, 01.04.2012

Rafet Tok Ayhan Uysal Oktay Burma İbrahim Hanifi Özercan

Abstract

Objective: This study explores the influence of vasodilators on the apoptotic activity related to endothelial damage caused by mechanical distension of the saphenous vein. Materials and Methods: Thirty-two patients without varicose veins undergoing coronary artery bypass graft operations were divided into four equal groups. Group I (control) Normal Saline, group II Papaverine group III Verapamil and group IV Nitroglycerine solutions. Percentange of immunohistochemical reactivity, pro-apoptotic bax and kaspaz-9 and anti-apoptotic bcl-2 were measured in the samples. Results: The percentage of cells which were positive for Bax (+) In Group I (Control) was 85.00 ± 6.89, Group II (Papaverine) 64.63 ± 8.73, Group III (Verapamil) 23.75 ± 4.20 and Group IV (Nitroglycerine) 22.00 ± 7.69. While there was no statistical difference between verepamil and nitroglycerin groups, they both had significantly decreased percentage of cells with proapoptotic Bax (+) compared to the control and the papaverine groups (p

Keywords

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References

  • O’Brien J, Michael L, Lambert L, Yi S, Dian W. Early injury
  • to media after saphenous vein grafting. Ann Thorac Surg 1998: 65; 1273-8.
  • Rodriguez E, Erica H, Lambert BS, Michael G, John D. Contractile smooth muscle cell apoptosis early after saphenous vein grafting. Ann Thorac Surg 2000: 70; 1145-52.
  • Galea J, Armstrong J, Cooper G, Crossman DC, Francis SE, Holt CM. Alterations in c-fos expression, cell proliferasyon and apoptozis pressure distented human saphenous vein. Cardiovasculer Research 1999: 44; 436-48.
  • Cornelissen J, Armstrong J, Holt CM. Mechanical stretch induces phosphorylation of p38 and apoptozis in human saphenous vein Vasc Biol 2004: 24; 451-6.
  • Karabulut H, Karabulut 0, Arbak S, Şan T, Sokullu O, Koruk- çu A. Koroner bypass cerrahisinde kullanılan safen veninin hazırlanmasında endotel hasarı: Işık ve elektron mikroskopik inceleme Türk Kardiyol Dern Arş 1998: 26; 416-24.
  • Evans A, Silari P. Jarzembowski T. Navarro A. Zhang W, Benedetti E, et al. Effect of preservation media on cellular apoptozis in autologous saphenous vein grefts procured for coronary revascularization. J Surg Res 2004: 114; 222-55.
  • Fırat Tıp Dergisi 2012; 17(2): proof
  • Perroulaz G. Morphology and structure of the venous wall. Ramelet AA, Monti M, Bounameaux H, Buchheim G, Capasso P (editors). Phlebology The Guide. 4.baskı, Paris, France Elsevier SAS 1999: 51-8.
  • Jesuthasan LSB, Angus JA, Rosenfeldt FL . In vitro comparison of glyceryl trinitrate-verapamil with other dilators of human saphenous vein. ANZ Journal of Surgery 2003: 73; 313-20.
  • Walsh K, Smith RC, Kim HS. Vascular Cell Apoptosis in Remodeling Restenosis, and Plaque Rupture. Circ Res 2000: 87; 184-8.
  • Özsin KK, Rahman A, Özercan İH, Burma O, Uysal A. The Role of Apoptosis in Lower Extremity Primary Varicose Veins. J Cardiovasc Sci 2008: 20; 184-91.
  • Baumann FG, Catinella FP, Cunningham JN, Spencer FC. Vein contraction and smooth muscle cell extensions as causes of endothelial damage during graft preparation. Ann Surg 1981: 194; 199-211.
  • Ruobos N, Rosenfeldt FL, Richards SM, Conyers RA, Davis BB. Improved preservation of saphenous vein grafts by the use of glyceryl trinitrate-verapamil solution during harvesting. Circulation 1995: 92; 31-6.
  • Roberts AJ, Hay DA, Mehta JL, Mehta P, Roy L, Faro RS, et al. Biochemical and ultrastructural integrity of saphenous vein conduit during coronar artery bypass grafting. Preliminary results of the effect of papaverine. J Thorac Surgery Surg 1984: 88; 39-48.
  • Gao YJ, Stead S, Lee RM. Papaverine induces apoptosis in vascular endothelial and smooth muscle cells. Life Sci2002: 70; 2675-85.
  • Gönderilme Tarihi: 28.02.2012

Mekanik Distansiyon Uygulanan Safen Vende Apopitozis Üzerine Vazodilatatör Solüsyonların Etkisi

Year 2012, Volume: 17 Issue: 2, 94 - 99, 01.04.2012

Rafet Tok Ayhan Uysal Oktay Burma İbrahim Hanifi Özercan

Abstract

Amaç: Bu çalışmamızda, safen vende mekanik distansiyonun sebep olduğu endotelyum harabiyetinde, apopitotik aktivitenin rolünü araştırmayı amaçladık. Gereç ve Yöntem: Çalışmaya her iki cinsten, koroner arter bypass greft operasyonuna alınan, venöz yetmezliği olmayan 32 hasta dahil edildi. Herbir grupta 8 hasta olmak üzere hastalar 4 gruba ayrıldı; Grup1 (Kontrol), Grup 2 (Papaverin), Grup 3 (Verapamil) ve Grup 4 (Nitrogliserin). Herbir grubun VSM'larından doku örnekleri alındı. Doku kesitlerinde immünohistokimyasal boyama ile proapopitotik bax, kaspaz-9 ve antiapopitotik bcl-2 boyanma yüzdeleri araştırıldı. Bulgular: Gruplarda yer alan safen venlerde proapopitotik Bax (+) hücre yüzdeleri sırasıyla 85.00 ± 6.89, 64.63 ± 8.73, 23.75 ± 4.20 ve 22.00 ± 7.69 olarak saptandı. Verapamil ve nitrogliserin grupları arasında istatistiksel bir fark bulunmazken, bu gruplardaki değerler kontrol ve papaverin grubuna göre anlamlı derecede düşük bulundu (p

Keywords

Apopitozis Vazodilatatör Safen ven

References

  • O’Brien J, Michael L, Lambert L, Yi S, Dian W. Early injury
  • to media after saphenous vein grafting. Ann Thorac Surg 1998: 65; 1273-8.
  • Rodriguez E, Erica H, Lambert BS, Michael G, John D. Contractile smooth muscle cell apoptosis early after saphenous vein grafting. Ann Thorac Surg 2000: 70; 1145-52.
  • Galea J, Armstrong J, Cooper G, Crossman DC, Francis SE, Holt CM. Alterations in c-fos expression, cell proliferasyon and apoptozis pressure distented human saphenous vein. Cardiovasculer Research 1999: 44; 436-48.
  • Cornelissen J, Armstrong J, Holt CM. Mechanical stretch induces phosphorylation of p38 and apoptozis in human saphenous vein Vasc Biol 2004: 24; 451-6.
  • Karabulut H, Karabulut 0, Arbak S, Şan T, Sokullu O, Koruk- çu A. Koroner bypass cerrahisinde kullanılan safen veninin hazırlanmasında endotel hasarı: Işık ve elektron mikroskopik inceleme Türk Kardiyol Dern Arş 1998: 26; 416-24.
  • Evans A, Silari P. Jarzembowski T. Navarro A. Zhang W, Benedetti E, et al. Effect of preservation media on cellular apoptozis in autologous saphenous vein grefts procured for coronary revascularization. J Surg Res 2004: 114; 222-55.
  • Fırat Tıp Dergisi 2012; 17(2): proof
  • Perroulaz G. Morphology and structure of the venous wall. Ramelet AA, Monti M, Bounameaux H, Buchheim G, Capasso P (editors). Phlebology The Guide. 4.baskı, Paris, France Elsevier SAS 1999: 51-8.
  • Jesuthasan LSB, Angus JA, Rosenfeldt FL . In vitro comparison of glyceryl trinitrate-verapamil with other dilators of human saphenous vein. ANZ Journal of Surgery 2003: 73; 313-20.
  • Walsh K, Smith RC, Kim HS. Vascular Cell Apoptosis in Remodeling Restenosis, and Plaque Rupture. Circ Res 2000: 87; 184-8.
  • Özsin KK, Rahman A, Özercan İH, Burma O, Uysal A. The Role of Apoptosis in Lower Extremity Primary Varicose Veins. J Cardiovasc Sci 2008: 20; 184-91.
  • Baumann FG, Catinella FP, Cunningham JN, Spencer FC. Vein contraction and smooth muscle cell extensions as causes of endothelial damage during graft preparation. Ann Surg 1981: 194; 199-211.
  • Ruobos N, Rosenfeldt FL, Richards SM, Conyers RA, Davis BB. Improved preservation of saphenous vein grafts by the use of glyceryl trinitrate-verapamil solution during harvesting. Circulation 1995: 92; 31-6.
  • Roberts AJ, Hay DA, Mehta JL, Mehta P, Roy L, Faro RS, et al. Biochemical and ultrastructural integrity of saphenous vein conduit during coronar artery bypass grafting. Preliminary results of the effect of papaverine. J Thorac Surgery Surg 1984: 88; 39-48.
  • Gao YJ, Stead S, Lee RM. Papaverine induces apoptosis in vascular endothelial and smooth muscle cells. Life Sci2002: 70; 2675-85.
  • Gönderilme Tarihi: 28.02.2012
There are 17 citations in total.

Details

Primary Language Turkish
Journal Section Articles
Authors

Rafet Tok This is me

Ayhan Uysal This is me

Oktay Burma This is me

İbrahim Hanifi Özercan This is me

Publication Date April 1, 2012
Published in Issue Year 2012 Volume: 17 Issue: 2

Cite

APA Tok, R., Uysal, A., Burma, O., Özercan, İ. H. (2012). Mekanik Distansiyon Uygulanan Safen Vende Apopitozis Üzerine Vazodilatatör Solüsyonların Etkisi. Fırat Tıp Dergisi, 17(2), 94-99.
AMA Tok R, Uysal A, Burma O, Özercan İH. Mekanik Distansiyon Uygulanan Safen Vende Apopitozis Üzerine Vazodilatatör Solüsyonların Etkisi. Fırat Tıp Dergisi. April 2012;17(2):94-99.
Chicago Tok, Rafet, Ayhan Uysal, Oktay Burma, and İbrahim Hanifi Özercan. “Mekanik Distansiyon Uygulanan Safen Vende Apopitozis Üzerine Vazodilatatör Solüsyonların Etkisi”. Fırat Tıp Dergisi 17, no. 2 (April 2012): 94-99.
EndNote Tok R, Uysal A, Burma O, Özercan İH (April 1, 2012) Mekanik Distansiyon Uygulanan Safen Vende Apopitozis Üzerine Vazodilatatör Solüsyonların Etkisi. Fırat Tıp Dergisi 17 2 94–99.
IEEE R. Tok, A. Uysal, O. Burma, and İ. H. Özercan, “Mekanik Distansiyon Uygulanan Safen Vende Apopitozis Üzerine Vazodilatatör Solüsyonların Etkisi”, Fırat Tıp Dergisi, vol. 17, no. 2, pp. 94–99, 2012.
ISNAD Tok, Rafet et al. “Mekanik Distansiyon Uygulanan Safen Vende Apopitozis Üzerine Vazodilatatör Solüsyonların Etkisi”. Fırat Tıp Dergisi 17/2 (April 2012), 94-99.
JAMA Tok R, Uysal A, Burma O, Özercan İH. Mekanik Distansiyon Uygulanan Safen Vende Apopitozis Üzerine Vazodilatatör Solüsyonların Etkisi. Fırat Tıp Dergisi. 2012;17:94–99.
MLA Tok, Rafet et al. “Mekanik Distansiyon Uygulanan Safen Vende Apopitozis Üzerine Vazodilatatör Solüsyonların Etkisi”. Fırat Tıp Dergisi, vol. 17, no. 2, 2012, pp. 94-99.
Vancouver Tok R, Uysal A, Burma O, Özercan İH. Mekanik Distansiyon Uygulanan Safen Vende Apopitozis Üzerine Vazodilatatör Solüsyonların Etkisi. Fırat Tıp Dergisi. 2012;17(2):94-9.