Effects of Polychlorinated Biphenyls on Catecholaminergic Neurotransmitter Levels in Cortex, Striatum, Hippocampus and Hypothalamus of Rat

Year 2007, Volume: 12 Issue: 2, 88 - 92, 01.04.2007

Mehmet Aydın Sinan Canpolat Ayşe Doğan Seyran Özgür Bulmuş Bayram Yılmaz

Abstract

Objectives: Polychlorinated biphenyls (PCBs) cause environmental chemical contamination and tend to bio-accumulate in living organisms due to their lipophylic properties. In this study, we have investigated effects two different Aroclor mixtures (Aroclor A1221 and A1254) on catecholamine levels in the cortex, striatum, hippocampus and hypothalamus in female rats. Material and Methods: Total 25 adult female Wistar rats were divided in three groups and subcutaneously injected with vehicle alone (4% dimethyl sulphoxide), A1221 (10 mg/kg) and A1254 (10 mg/kg) every other day for a period of 46 days. At the end of the experiments, animals were decapitated and brains rapidly removed. Cortex, striatum, hippocampus and hypothalamus regions were isolated, and homogenized. Brain noradrenalin, dopamine and metabolites concentrations were determined by HPLC-ECD method. Results: A1221 administration significantly increased levels of noradrenalin and its metabolite (dihydroxyphenyl glycol, DHPG) in cortex and striatum (p

Keywords

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Poliklorlu Bifenillerin Sıçan Korteks, Hipokampus, Striatum ve Hipotalamus Dokusunda Katekolaminerjik Nörotransmiter Düzeylerine Etkileri

Abstract

Giriş: Poliklorlu bifeniller (PCB) endüstriyel amaçla üretilen organik klorlu bileşiklerdir. Doğada yaygın kimyasal kirlenmeye yol açtıkları ve lipofilik özellikleri nedeniyle canlı organizmada birikme eğilimi gösterdikleri bilinmektedir. Bu çalışmada, farklı iki PCB karışımı olan Aroklor 1221 (A1221) ve Aroklor 1254 (A1254)'ün dişi sıçanlarda korteks, hipokampus, striatum ve hipotalamus dokularında katekolamin düzeyleri üzerine etkilerinin araştırılması amaçlandı. Gereç ve Yöntem: Toplam 25 Wistar dişi sıçan üç gruba ayrıldı ve sırasıyla; taşıt solüsyonu olarak dimetil sülfoksit (%4), A1221 (10 mg/kg) ve A1254 (10 mg/kg) subkutan yolla gün aşırı olarak ve 46 gün süreyle uygulandı. Deneylerin sonunda hayvanlar dekapite edildi ve beyin dokuları hızla alındı. Korteks, hipotalamus, striatum ve hipokampus bölgeleri çıkartıldıktan sonra homojenize edildi. Doku noradrenalin, dopamin ve metabolit düzeyleri HPLC-ECD metodu kullanılarak ölçüldü. Bulgular: A1221; noradrenalin ve onun metaboliti olan dihidroksifenil glikol (DHPG) düzeylerini korteks ve striatumda artırırken (p

Keywords

PCB, Aroclor, noradrenalin, dopamin ve HPLC-ECD

References

• Hansen LG. The ortho side of PCBs: occurrence and disposition. 1999; Kluwer Academic Publishers.
• Carpenter DO, Arcaro KF, Bush B, Niemi WD, Pang S ve Vakharia DD. Human health and chemical mixtures: an overview. Environ Health Perspect 1998; 106: 1263-1270.
• Carpenter DO. Polychlorinated biphenyls and human health. Int J Occup Med Environ Health 1998; 11: 291-303.
• Kamrin, M. A., ve Ringer, R. K. PCB residues in mammals: A review. Toxicol Environ Chem 1994; 41: 63–84.
• Carpenter DO. Environmental Contaminants and Human Health: The Health Effects of Persistent Toxic Substances. Fırat Tıp Dergisi 2005; 10: 1-4.
• Telli-Karakoc F, Tolun L, Henkelmann B, ve ark. Polycyclic aromatic hydrocarbons (PAHs) and polychlorinated biphenyls (PCBs) distributions in the Bay of Marmara sea: Izmit Bay. Environ Pollut 2002; 119: 383-397.
• Cok I, Donmez MK ve Karakaya AE. Levels and trends of chlorinated pesticides in human breast milk from Ankara residents: comparison of concentrations in 1984 and 2002,” Bull Environ Contam Toxicol 2004; 72: 522-529.
• Silberhorn EM, Glauert HP ve Robertson LW. Carcinogenicity of polyhalogenated biphenyls: PCBs and PBBs. Crit Rev Toxicol 1990; 20: 439–496.
• James RC, Busch H, Tamburro CH, ve ark. Polychlorinated biphenyl exposure and human disease. J Occup Med 1993; 35: 136–148.
• Tilson HA ve Codavanty PRS. The neurotoxicity of polychlorinated biphenyls. Neurotoxicology 1998; 19: 517–525.
• Seegal, R. F. Epidemiological and laboratory evidence of PCB- induced neurotoxicity. Crit Rev Toxicol 1996; 26: 109–737.
• Kimbrough RD ve Krouskas CA. Human exposure to polychlorinated biphenyls and health effects: a critical synopsis. Toxicol Rev 2003; 22, 217-33.
• Ulbrich B ve Stahlmann R. Developmental toxicity of polychlorinated biphenyls (PCBs): a systematic review of experimental data. Arch Toxicol 2004; 78: 252-68.
• Kutlu S, Colakoglu N, Halifeoglu I, ve ark. Comparative evaluation of hepatotoxic and nephrotoxic effects of aroclors 1221 and 1254 in female rats. Cell Biochem Funct 2005; PMID: 16180246, In Press.
• Levin ED, Shantz SL, ve Bowman RE. Delayed spatial alteration deficits from perinatal PCB exposure in monkeys. Arch Toxicol 1988; 62: 267–273.
• Shantz SL, Levin ED ve Bowman RE. Long-term neurobehavioral effects of perinatal PCB exposure in monkeys. Environ Toxicol Chem 1992; 10: 747–756.
• Rogan WJ ve Gladen BC. PCBs, DDE, and child development at 18 and 24 months. Ann Epidemiol 1991; 5: 407-13.
• Hsu ST, Ma CI, Hsu SK, ve ark. Discovery and epidemiology of PCB poisoning in Taiwan: a four-year followup. Environ Health Perspect 1985; 59:5-10.
• Jacobson JL ve Jacobson SW. Intellectual impairment in children exposed to polychlorinated biphenyls in utero. N Engl J Med 1996; 335:783–789.
• Johnson BL ve De Rosa CT. Public health implications.Environ Res 1999; 80: S246–S248.
• Holene E, Nafstad I, Skaare JU ve Sagvolden T. Behavioural hyperactivity in rats following postnatal exposure to sub-toxic doses of polychlorinated biphenyl congeners 153 and 126. Behav Brain Res 1988; 94: 213–224.
• Schantz SL, Moshtaghian J ve Ness DK. Spatial learning deficits in adult rats exposed to ortho-substituted PCB congeners during gestation and lactation. Fundam Appl Toxicol 1995; 26: 117–126.
• Seegal RF, Bush B, ve Brosch KO. Polychlorinated biphenyls induce changes in brain norepinephrine concentrations in adult rats. Neurotoxicology 1985; 6: 13–24.
• Seegal RF, Brosch KO, ve Bush B. Regional alterations in serotonin metabolism induced by oral exposure of rats to polychlorinated biphenyls. Neurotoxicology 1986; 7: 155–166.
• Seegal RF, Bush B, ve Brosch KO. Sub chronic exposure of the adult rat to Aroclors 1254 yields regionally specific changes in central dopaminergic function. Neurotoxicology 1991; 12: 55–66.
• Chu I, Villeneuve DC, Yagminas A, Lecavalier P, ve ark. Toxicity of 2,2',4,4',5,5'-hexachlorobiphenyl in rats: effects following 90-day oral exposure. J Appl Toxicol 1996; 16:121– 128
• Chishti MA, Fisher JP ve Seegal RF. Aroclors 1254 and 1260 reduce dopamine concentrations in rat striatal slices. Neurotoxicology 1996; 17: 653-60.
• Khan IA ve Thomas P. Aroclor 1254-induced alterations in hypothalamic monoamine metabolism in the Atlantic croaker (Micropogonias unulatus): correlation with pituitary gonadotropin release. Neurotoxicology 1997; 18: 553–560.
• Zahalka EA, Ellis DH, Goldey ES, Stanton ME ve Lau C. Perinatal exposure to polychlorinated biphenyls Aroclor 1016 or 1254 did not alter brain catecholamines nor delayed alternation performance in Long-Evans rats. Brain Res Bull 2001; 55: 487- 500.
• Corrigan FM, Murray L, Wyatt CL ve Shore RF. Diorthosubstituted polychlorinated biphenyls in caudate nucleus in Parkinson’s disease. Exp Neurol 1998: 150: 339–342.
• Steenland K, Hein MJ, Cassinelli RT ve ark. Polychlorinated biphenyls and neurodegenerative disease mortality in an occupational cohort. Epidemiology 2006; 17: 8-13.
• Dean CE Jr, Benjamin SA, Chubb LS, Tessari JD ve Keefe TJ. Nonadditive hepatic tumor promoting effects by a mixture of two structurally different polychlorinated biphenyls in female rat livers. Toxicol Sci 2002; 66: 54-61.
• Yılmaz B, Gilmore DP ve Wilson CA. Inhibition of the pre- ovulatory LH surge in the rat by central noradrenergic mediation: Involvement of an anaesthetic (urethane) and opioid receptor agonists. Biogenic Amines 1996; 12, 423-435.
• Fonnum F, Mariussen E ve Reistad T. Molecular mechanisms involved in the toxic effects of polychlorinated biphenyls (PCBs) and brominated flame retardants (BFRs). J Toxicol Environ Health A 2006; 69: 21-35.
• Seegal, R. F. Epidemiological and laboratory evidence of PCB- induced neurotoxicity. Crit. Rev. Toxicol. 1996; 26: 109–737.
• Bemis, J. C. ve Seegal, R. F. Polychlorinated biphenyls and methylmercury alter intracellular calcium concentrations in rat cerebellar granule cells. Neurotoxicology. 2000; 21:1123-34
• Choksi NY, Kodavanti PR, Tilson HA ve Booth RG. Effects of polychlorinated biphenyls (PCBs) on brain tyrosine hydroxylase activity and dopamine synthesis in rats. Fundam Appl Toxicology 1997; 39: 76-80.
• Mariussen E, Morch Andersen J ve Fonnum F. The effect of polychlorinated biphenyls on the uptake of dopamine and other neurotransmitters into rat brain synaptic vesicles. Toxicol Appl Pharmacology 1999; 161: 274-82.
• Bemis JC ve Seegal RF. PCB-induced inhibition of the vesicular monoamine transporter predicts reductions in synaptosomal dopamine content. Toxicol Sci 2004; 80: 288-95
• Richardson JR ve Miller GW. Acute exposure to aroclor 1016 or 1260 differentially affects dopamine transporter and vesicular monoamine transporter 2 levels. Toxicol Lett 2004; 148: 29-40.
• Lee DW ve Opanashuk LA. Polychlorinated biphenyl mixture aroclor 1254-induced oxidative stress plays a role in dopaminergic cell injury. Neurotoxicology 2004; 25: 925-39.
• Seegal RF, Brosch KO ve Okoniewski R. The degree of PCB chlorination determines whether the rise in urinary homovanillic acid production in rats is peripheral or central in origin. Toxicol Appl Pharmacol 1988; 96: 560-564
• van Larebeke N, Hens L, Schepens P ve ark. The Belgian PCB and dioxin incident of January-June 1999: exposure data and potential impact on health. Environ Health Perspect 2001; 109: 265-73.
• Steenland K, Hein MJ, Cassinelli RT ve ark. Polychlorinated biphenyls and neurodegenerative disease mortality in an occupational cohort. Epidemiology 2006; 17: 8-13
• Kabul Tarihi: 05.09.2006