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Effect of Ghrelin on Pain Threshold in Mice

Year 2005, Volume: 10 Issue: 3, 89 - 91, 01.06.2005

Selim Kutlu Mete Ozcan Sinan Canpolat Süleyman Sandal Mehmet Aydın Haluk Kelestimur

Abstract

Objectives: Ghrelin, a novel growth hormone-releasing peptide, was isolated from the rat stomach as an endogenous ligand for the growth hormone secretagogues receptor. The present study was planned to determine whether ghrelin affects pain threshold in mice. Materials and Methods: Adult male BALB/C mice weighing 25–30g were used in this study. The hot plate test was conducted by placing the mouse on a metal surface maintained at 50±0.1ºC by using hot plate analgesia meter. The latency to jumping or licking a hind paw was recorded as nociceptive threshold. Animals were allowed to acclimate to the hot plate for a period of 1 week prior to the experiment. Different doses of ghrelin were intraperitoneally administered to the animals after control latencies. Control group received saline alone. Hot plate test were performed in all animals individually in 30 th, 60 th, 90 th and 120 th minutes after injection. Pain threshold values were determined and analyzed by Mann-Whitney U Test and Wilkoxon Sign Ranks Test. Results: Ghrelin didn't affect pain threshold throughout the experiment in 0.3pmol and 1pmol doses compared to control values. There were significant decreases in pain threshold when it is given in a dose of 3pmol in 30 th and 60 th minutes (p

Keywords

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References

  • Weikel JC, Wichniak A, Ising M, et al. Ghrelin promotes slow- wave sleep in humans. Am J Physiol Endocrinol Metab 2003; 284: 407-15.
  • McLaughlin T, Abbasi F, Lamendola C, Frayo RS, Cummings DE. Plasma ghrelin concentrations are decreased in insulin- resistant obese adults relative to equally obese insulin-sensitive controls. J Clin Endocrinol Metab 2004; 89: 1630-5.
  • Ariyasu H, Takaya K, Hosoda H, et al. Delayed short-term secretory regulation of ghrelin in obese animals: evidenced by a specific RIA for the active form of ghrelin. Endocrinol 2002; 143: 3341-50.
  • Glavaski-Joksimovic A, Jeftinija K, Scanes CG, Anderson LL, Jeftinija S. Stimulatory effect of ghrelin on isolated porcine somatotropes. Neuroendocrinol 2003; 77: 367-79.
  • Kohno D, Gao HZ, Muroya S, Kikuyama S, Yada T. Ghrelin directly interacts with neuropeptide-Y-containing neurons in the rat arcuate nucleus: Ca2+ signaling via protein kinase A and N- type channel-dependent mechanisms and cross-talk with leptin and orexin. Diabetes 2003; 52: 948-56.
  • Schwartz JC, Arrang JM, Garbarg M, Pollard H, Ruat M. Histaminergic transmission in the mammalian brain. Physiol Rev 1991; 71: 1-51
  • Bajic D, VanManh Hoang Q, Nakajima S, Nakajima Y. Dissociated tuberomammillary nucleus of rats: culture methods and ghrelin effects. J Neurosci Met 2004; 132: 177-84. cultures from the
  • Glick SD, Crane LA. Opiate-like and abstinence-like effects of intracerebral histamine administration in rats. Nature 1978; 273: 547-9.
  • Thoburn KK, Hough LB, Nalwalk JW, Mischler SA. Histamine- induced modulation of nociceptive responses. Pain 1994; 58: 29- 37.
  • Chung YH, Miyake H, Kamei C, Tasaka K. Analgesic effect of histamine induced by intracerebral injection into mice. Agents Actions 1984; 15: 137-42.
  • Malmberg-Aiello P, Lamberti C, Ghelardini C, Giotti A, Bartolini A.Role of histamine in rodent antinociception. Br J Pharmacol 1994; 111: 1269-79.
  • Lamberti C, Bartolini A, Ghelardini C, Malmberg-Aiello P. Investigation into the role of histamine receptors in rodent antinociception. Pharmacol Biochem Behav 1996; 3: 567-74.
  • Andersen E, Dafny N. An ascending serotonergic pain modulation pathway from the dorsal raphe nucleus to the parafascicularis nucleus of the thalamus. Brain Res 1983; 269: 57-67.
  • Brunetti L, Recinella L, Orlando G, et al. Effects of ghrelin and amylin on dopamine, norepinephrine and serotonin release in the hypothalamus. Eur J Pharmacol 2002; 454: 189-92
  • Carlini VP, Varas MM, Cragnolini AB, et al. Differential role of the hippocampus, amygdala, and dorsal raphe nucleus in regulating feeding, memory, and anxiety-like behavioral responses to ghrelin. Biochem Biophys Res Commun 2004; 313: 635-41.
  • Kabul Tarihi:26.05.2005

Farelerde Ağri Eşiği Üzerine Grelinin Etkisi

Year 2005, Volume: 10 Issue: 3, 89 - 91, 01.06.2005

Selim Kutlu Mete Ozcan Sinan Canpolat Süleyman Sandal Mehmet Aydın Haluk Kelestimur

Abstract

Amaç: Yeni bir büyüme hormonu salgılatıcı peptit olan grelin, büyüme hormonu salgılatıcı reseptörlerin endojen bir ligandı olarak sıçan midesinden izole edilmiştir. Bu çalışma, grelinin farelerde ağrı eşiği üzerindeki olası etkilerini belirlemek amacıyla planlanmıştır. Gereç ve Yöntem: Çalışmada 25-30 gram ağırlığında yetişkin erkek BALB/C fareler kullanıldı. Hot plate testi, farelerin analjezimetrenin 50±0.1ºC'deki metal yüzeyine bırakıldıkları andan itibaren, ayaklarını hızla çırptıkları veya yaladıkları süre saniye olarak ağrı eşiği değeri için kaydededilerek uygulandı.. Deneylerden önce hayvanlar 1 hafta süreyle hot plate'e alıştırıldı. Deney günü, analjezimetrede kontrol kayıtları alındıktan hemen sonra, intraperitoneal yolla farlı dozlardaki grelin uygulamaları yapıldı. Kontrol grubuna serum fizyolojik verildi. Bütün hayvanlara enjeksiyonlardan 30, 60, 90 ve 120 dakika sonra test uygulandı. Ağrı eşiği değerleri belirlendi ve Mann-Whitney U Test ve Wilkoxon Sign Ranks Test kullanılarak analiz edildi. Bulgular: Grelin kontrol grubuyla karşılaştırıldığında 0.3 pmol ve 1 pmol dozlarında ağrı eşiğini etkilemedi. 3 pmol dozda ise, 30. ve 60. dakikalarda ağrı eşiğinde belirgin azalmalar ortaya çıktı (p

Keywords

Grelin ağrı eşiği hot plate ve fare

References

  • Weikel JC, Wichniak A, Ising M, et al. Ghrelin promotes slow- wave sleep in humans. Am J Physiol Endocrinol Metab 2003; 284: 407-15.
  • McLaughlin T, Abbasi F, Lamendola C, Frayo RS, Cummings DE. Plasma ghrelin concentrations are decreased in insulin- resistant obese adults relative to equally obese insulin-sensitive controls. J Clin Endocrinol Metab 2004; 89: 1630-5.
  • Ariyasu H, Takaya K, Hosoda H, et al. Delayed short-term secretory regulation of ghrelin in obese animals: evidenced by a specific RIA for the active form of ghrelin. Endocrinol 2002; 143: 3341-50.
  • Glavaski-Joksimovic A, Jeftinija K, Scanes CG, Anderson LL, Jeftinija S. Stimulatory effect of ghrelin on isolated porcine somatotropes. Neuroendocrinol 2003; 77: 367-79.
  • Kohno D, Gao HZ, Muroya S, Kikuyama S, Yada T. Ghrelin directly interacts with neuropeptide-Y-containing neurons in the rat arcuate nucleus: Ca2+ signaling via protein kinase A and N- type channel-dependent mechanisms and cross-talk with leptin and orexin. Diabetes 2003; 52: 948-56.
  • Schwartz JC, Arrang JM, Garbarg M, Pollard H, Ruat M. Histaminergic transmission in the mammalian brain. Physiol Rev 1991; 71: 1-51
  • Bajic D, VanManh Hoang Q, Nakajima S, Nakajima Y. Dissociated tuberomammillary nucleus of rats: culture methods and ghrelin effects. J Neurosci Met 2004; 132: 177-84. cultures from the
  • Glick SD, Crane LA. Opiate-like and abstinence-like effects of intracerebral histamine administration in rats. Nature 1978; 273: 547-9.
  • Thoburn KK, Hough LB, Nalwalk JW, Mischler SA. Histamine- induced modulation of nociceptive responses. Pain 1994; 58: 29- 37.
  • Chung YH, Miyake H, Kamei C, Tasaka K. Analgesic effect of histamine induced by intracerebral injection into mice. Agents Actions 1984; 15: 137-42.
  • Malmberg-Aiello P, Lamberti C, Ghelardini C, Giotti A, Bartolini A.Role of histamine in rodent antinociception. Br J Pharmacol 1994; 111: 1269-79.
  • Lamberti C, Bartolini A, Ghelardini C, Malmberg-Aiello P. Investigation into the role of histamine receptors in rodent antinociception. Pharmacol Biochem Behav 1996; 3: 567-74.
  • Andersen E, Dafny N. An ascending serotonergic pain modulation pathway from the dorsal raphe nucleus to the parafascicularis nucleus of the thalamus. Brain Res 1983; 269: 57-67.
  • Brunetti L, Recinella L, Orlando G, et al. Effects of ghrelin and amylin on dopamine, norepinephrine and serotonin release in the hypothalamus. Eur J Pharmacol 2002; 454: 189-92
  • Carlini VP, Varas MM, Cragnolini AB, et al. Differential role of the hippocampus, amygdala, and dorsal raphe nucleus in regulating feeding, memory, and anxiety-like behavioral responses to ghrelin. Biochem Biophys Res Commun 2004; 313: 635-41.
  • Kabul Tarihi:26.05.2005
There are 16 citations in total.

Details

Primary Language Turkish
Journal Section Articles
Authors

Selim Kutlu This is me

Mete Ozcan This is me

Sinan Canpolat This is me

Süleyman Sandal This is me

Mehmet Aydın This is me

Haluk Kelestimur This is me

Publication Date June 1, 2005
Published in Issue Year 2005 Volume: 10 Issue: 3

Cite

APA Kutlu, S., Ozcan, M., Canpolat, S., Sandal, S., et al. (2005). Farelerde Ağri Eşiği Üzerine Grelinin Etkisi. Fırat Tıp Dergisi, 10(3), 89-91.
AMA Kutlu S, Ozcan M, Canpolat S, Sandal S, Aydın M, Kelestimur H. Farelerde Ağri Eşiği Üzerine Grelinin Etkisi. Fırat Tıp Dergisi. June 2005;10(3):89-91.
Chicago Kutlu, Selim, Mete Ozcan, Sinan Canpolat, Süleyman Sandal, Mehmet Aydın, and Haluk Kelestimur. “Farelerde Ağri Eşiği Üzerine Grelinin Etkisi”. Fırat Tıp Dergisi 10, no. 3 (June 2005): 89-91.
EndNote Kutlu S, Ozcan M, Canpolat S, Sandal S, Aydın M, Kelestimur H (June 1, 2005) Farelerde Ağri Eşiği Üzerine Grelinin Etkisi. Fırat Tıp Dergisi 10 3 89–91.
IEEE S. Kutlu, M. Ozcan, S. Canpolat, S. Sandal, M. Aydın, and H. Kelestimur, “Farelerde Ağri Eşiği Üzerine Grelinin Etkisi”, Fırat Tıp Dergisi, vol. 10, no. 3, pp. 89–91, 2005.
ISNAD Kutlu, Selim et al. “Farelerde Ağri Eşiği Üzerine Grelinin Etkisi”. Fırat Tıp Dergisi 10/3 (June 2005), 89-91.
JAMA Kutlu S, Ozcan M, Canpolat S, Sandal S, Aydın M, Kelestimur H. Farelerde Ağri Eşiği Üzerine Grelinin Etkisi. Fırat Tıp Dergisi. 2005;10:89–91.
MLA Kutlu, Selim et al. “Farelerde Ağri Eşiği Üzerine Grelinin Etkisi”. Fırat Tıp Dergisi, vol. 10, no. 3, 2005, pp. 89-91.
Vancouver Kutlu S, Ozcan M, Canpolat S, Sandal S, Aydın M, Kelestimur H. Farelerde Ağri Eşiği Üzerine Grelinin Etkisi. Fırat Tıp Dergisi. 2005;10(3):89-91.