Determination of Antibacterial Efficacies of Plant Extracts on Tomato Bacterial Speck Disease

Year 2015, Volume: 44 Issue: 1-2-3, 1 - 10, 30.01.2016

Kubilay Kurtuluş Baştaş

Abstract

Bacterial
speck caused by Pseudomonas syringae pv. tomato is an economically important disease of tomato. The aim of this study was to evaluate the
effectiveness of some medicinal and
aromatic plant extracts on tomato seeds (cv. H-2274) and plants against P. s. pv. tomato
in vitro and in vivo. The extracts obtained from leaves, stems, seeds and
fruits of Juglans regia, Lavandula
officinalis, Eucalyptus globulus, Rosmarinus officinalis, Olea
europaea, Tilia tomentosa,
Jatropha curcas, Humulus lupulus, Nigella sativa, Trigonella
foenum-graecum, Cuminum cyminum,
Rhus coriaria, Daucus carota, Rosa canina and Moringa oleifera. Amongs the 15 different plants, three plant extracts
inhibited the growth of the pathogen with inhibition zone diameter ranging from
13 to 22 mm at 20% (w/v) in absolute methanol. Bacterial population on tomato seeds
after applications of the plant extracts at 5 doses was also determined.
Rhus coriaria and Eucalyptus
globulus extracts at 20% concentration completely inhibited the P. s. pv. tomato on tomato seeds. In
spite of the highest antibacterial effects of sumac and eucalyptus to the pathogen, there was no a negative effect on tomato
seed germination. The effects of E. globules,
R. coriaria and R. officinalis extracts
against the pathogen were examined in greenhose conditions and, the highest efficiency was achieved by E. globulus
(65.86%) on tomato. The peroxidase enzyme and total protein values have
shown an increase in the leaves following application of sumac, rosemary and
eucalyptus extracts before the inoculation with P. s. pv. tomato. This study indicated that some plant extracts
may be used in prevention programs to combat the bacterial speck disease on tomato.

Keywords

Pseudomonas syringae pv. tomato, tomato, plant extracts, biological control, peroxidase, protein

References

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