Melon necrotic spot virus (MNSV), A Newly Reported Virus Diseases for Turkey in Squash species

Year 2014, Volume: 43 Issue: 1-2-3, 1 - 6, 29.12.2014

Gökmen Koç Hakan Fıdan Saadettin Baloğlu

Abstract

The study was conducted on summer squash (Cucurbita pepo. Linneus, 1753)
grown in the plateau of Pozantı which is a county
of Adana, Turkey in 2013. During field surveys, systemic chlorotic rings and irregular yellowish dark
brown necrotic spots were observed on squash plants. For identification, 49 leaf
samples were tested by DAS-ELISA using polyclonal antiserum specific to viruses
commonly infecting cucurbits including Squash mosaic virus (SqMV), Cucumber mosaic
virus (CMV), Zucchini yellow mosaic virus(ZYMV), Watermelon
mosaic virus 2(WMV-2), Watermelon mosaic virus 1( WMV-1), Tomato
ringspot virus (ToRSV), Tobacco ringspot virus(TRSV) and Melon necrotic spot virus (MNSV).  Three
samples were tested positive for MNSV in DAS-ELISA and the results were
confirmed by RT-PCR using total RNA extracted from leaves (100mg). RT-PCR assay
was conducted by Sense MNS-C10 and antisense MNS-N40 primer pairs specific to
MNSV. As a result of RT-PCR, 485 bp cDNA bands were observed in 2% agarose gel.
While occurrence of the ZYMV  detected in
five samples, remaining  viruses
were  not detected in the tested samples.
In Turkey, the occurrence of MNSV was previously reported at melon field
samples by serological technique from Thrace region in 2006, also it was
detected in cucurbit breeding materials by serologically and molecular techniques
in Antalya in 2009. This study is not only first record of MNSV at squash in
Turkey but also provide evidence of uncontrolled distribution among both
species and regions.

Keywords

Squash, MNSV, Pozantı, DAS-ELISA, RT-PCR

References

• Bos, L., Van Dost, H. J. M., Huttigna, H. And Maat, D. Z. 1984. Further characterization of melon necrotic spot virus causing severe diseases in glasshouse cucumbers in the Netherlands and its control. Neth. J. Plant Pathol. 90:55-69. Compbell, R. N. 1996. Fungal transmision of plant viruses. Annu.Rev. Phytopathol. 34:87-108. Clark, M.F., And Adams, A.N., 1977. Charecteristic of Microplate method of Enzyme- linked Immunosorbent Assay for the Detection of Plant Viruses. J. Gen. Virol., 342 475-483. Coudriet Dl, Kishaba An, Bohn Gw 1981. Inheritance of resistance to muskmelon necrotic spot virus in a melon aphidresistant breeding line of muskmelon. J Am Soc Hort Sci 106:789–791 Di´Az Ja, Nieto C, Moriones E, Truniger V, Aranda Ma (2004) Molecular characterization of a Melon necrotic spot virus strain that overcomes the resistance in melon and non-host plants. Mol Plant Microbe Interact 6:668–675 Fidan, H., Unlu, A., Koc, G., Unlu, M. 2010 Turkiye’de Hıyar Bitkisinde Yeni Bir Virus Hastalıgı. Melon Necrotic Spot Virus (MNSV) VIII. Sebze Tarymy Sempozyumu 23 Haziran-26 Haziran 2010 - VAN / TURKYEVan S:490-494 Gu, Q.-S., Bao W.-H.,, Tian, Y.-P., Prins, M., Yang, H.-X., Lu, J., Liu, L.-F, and Penga, B.2008, Melon necrotic spot virüs newly reported in China, Plant Pathology (2008) 57, 765, Doi: 10.1111/j.1365-3059.2008.01847.x Hibi T, And Furuki, I., (1985) Melon necrotic spot virus. In: (eds) CMI/ AAB Descriptions of plant viruses. Association of applied biologists, Warwick, UK Jung, J. A., Kim, S. M., Kim, H. R., Lee, S. H., Choi, H. S., Park, J. W. And Kim, T. W. 2005. Diagnosis of Melon necrotic spot carmovirus by using RT-PCR and efficiency comparison with other diagnostic methods Plant Pathol. J. 21:434 (abstract). Kishi, K. 1966. Necrotic spot of melon, a new virüs disease. Ann.Phytopath. Soc. Japan 32:138-144. Koklu, G., And Yilmaz, O., 2006 Occurrence of cucurbit viruses on field-grown melon and watermelon in the Thrace region of Turkey Phytoprotection, vol. 87, n° 3, 2006, p. 123- 130. Matsuo K, Kameya-Iwaki M, Ota T 1991. Two new strains of Melon necrotic spot virus. Ann Phytopatol Soc Jpn 57:558–567 Morales, M., Orjeda, G., Nieto, C., Van Leeuwen, H., Monfor, A., Charpentier, M., Caboche, M., Arús, P., Puigdomènech, P., Aranda, M.A., Dogimont, C., Bendahmane, A., And Garcia-Mas, J., 2005. A physical map covering the nsv locus that confers resistance to Melon necrotic spot virus in melon (Cucumis melo L.). Theor Appl Genet (2005), DOI 10.1007/s00122-005-0019-y N. Astruc, J.F. Marcos, G. Macquaire, T. Candresse, V. Pallás, 1996. Studies on the diagnosis of hop stunt viroid in fruit trees: identification of new hosts and application of a nucleic acid extraction procedure based on non-organic solvents Eur. J. Plant Pathol., 102 (1996), pp. 837–846 Ozaslan, M., Aytekin, T., Bas, B., Kilic, H.I., Afacan D., And Dag,D.S. 2006. Virus Diseases of Cucurbits in Gaziantep-Turkey. Plant Pathology Journal, 5: 24-27. Riviere Cj, Rochon Dm 1990. Nucleotide sequence and genomic organization of Melon necrotic spot virus. J Gen Virol 71:1887– 1896 Sambrook, J., Fritsch, E.F. And Maniatis, T. 1989. Molecular Cloning: A Laboratory Manual, 2nd edn. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY. Yilmaz, M.A., Ozaslan, M., Ozaslan, D.,1989. Cucumber Vein Yellowing Virus in Cucurbiteceae in Turkey. Plant DiseaseT3 (7)z 610 (Abst). Yilmaz,M.A., Lecoq, H., Abak, K., Baloglu, S., Ve Sari, N., 1992. Ttirkiye'de Kabakgil Sebze TiirlerindeZarar Yapan Virusler. Tiirkiye I. Ulusal Bahge Bitkileri Kongresi, Ege UniversitesiZiraat Fakiiltesi, Bornova, izmir, Cilt I Sebze, (439- 442) 13-16 Ekim, 1992.