Melon necrotic spot virus (MNSV), A Newly Reported Virus Diseases for Turkey in Squash species

Year 2014, Volume: 43 Issue: 1-2-3, 1 - 6, 29.12.2014

Gökmen Koç , Hakan Fıdan , Saadettin Baloğlu

Abstract

The study was conducted on summer squash (Cucurbita pepo. Linneus, 1753)
grown in the plateau of Pozantı which is a county
of Adana, Turkey in 2013. During field surveys, systemic chlorotic rings and irregular yellowish dark
brown necrotic spots were observed on squash plants. For identification, 49 leaf
samples were tested by DAS-ELISA using polyclonal antiserum specific to viruses
commonly infecting cucurbits including Squash mosaic virus (SqMV), Cucumber mosaic
virus (CMV), Zucchini yellow mosaic virus(ZYMV), Watermelon
mosaic virus 2(WMV-2), Watermelon mosaic virus 1( WMV-1), Tomato
ringspot virus (ToRSV), Tobacco ringspot virus(TRSV) and Melon necrotic spot virus (MNSV).  Three
samples were tested positive for MNSV in DAS-ELISA and the results were
confirmed by RT-PCR using total RNA extracted from leaves (100mg). RT-PCR assay
was conducted by Sense MNS-C10 and antisense MNS-N40 primer pairs specific to
MNSV. As a result of RT-PCR, 485 bp cDNA bands were observed in 2% agarose gel.
While occurrence of the ZYMV  detected in
five samples, remaining  viruses
were  not detected in the tested samples.
In Turkey, the occurrence of MNSV was previously reported at melon field
samples by serological technique from Thrace region in 2006, also it was
detected in cucurbit breeding materials by serologically and molecular techniques
in Antalya in 2009. This study is not only first record of MNSV at squash in
Turkey but also provide evidence of uncontrolled distribution among both
species and regions.

Keywords

Squash, MNSV, Pozantı, DAS-ELISA, RT-PCR

References

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