A Novel Technique for The Recovery, Isolation and Preliminary Evaluation of Rhizoctonia solani Mycoparasites from Soil

Year 2017, Volume: 46 Issue: 1, 43 - 51, 14.11.2017

Hadi Aydın , Gülay Turhan

Abstract

This paper describes a
novel technique that allows detecting and isolating of R. solani mycoparasites subsist in soil incorporating the precolonized agar method and a
modified nutrient medium supplemented with tolclophos-methyl.
A great
number of potential biocontrol agents like Trichoderma and Gliocladium could be isolated into pure culture. Most of the isolates examined macroscopically and microscopically in
dual cultures had been recognized as mycoparasites.
By using this novel technique, various mycoparasitic isolates were obtained from soil. The majority of the
isolates were in the genera of Trichoderma
and Gliocladium. Fourteen Trichoderma species, Gliocladium roseum Bainier, Chaetomium sp., Geotrichum sp., Paecilomyces sp., Papulospora
sp. and a Spicaria sp. were determined as having mycoparasitic
activity against R. solani as well. Some of them possessed both
parasitic action and antifungal
antibiotic activity. This novel technique proved to be useful and showed
promise for studies of the ecology of biocontrol agents added to soil or seed.

Keywords

Isolation technique, mycoparasite, Rhizoctonia solani, Trichoderma, Gliocladium

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