Köpek Dişi Ayrığı Yaprak Beyazlaşma Fitoplazması (BGWL)'nın Köpek Dişi Ayrık Otundan Kök Kaynaşması Sistemi Yoluyla Buğday Bitkilerine (Triticum Spp.) Deneysel Olarak Taşınması

Year 2011, Volume: 40 Issue: 1-2-3, 51 - 56, 01.09.2011

Behçet Kemal Çağlar Serdar Satar Toufic Elbeaıno

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Experimentally Transmission of Bermuda Grass (Cynodon dactylon (L.) Pers.) White Leaf Phytoplasma to Wheat Plants by Root-Bridge Modality

Abstract

Bermuda grass white leaf phytoplasma (BGWL) was previously reported in Turkey. In the study, transmission of agents from Bermuda grass to wheat (Triticum spp.) plants was experimentally investigated by a root-bridge system in August 2012, Adana. Twelve Bermuda grass plants, infected with Candidatus Phytoplasma cynodontis, strain M6 from Turkey and showing symptoms of light green to yellow streaks on the leaves and bushy growth, were taken out from soil separately and transplanted to two plastic pots. Two hundred of wheat seeds were sown in to each pot between diseased Bermuda grass plants. After two months under net screen, wheat plants in the pots started to turn into yellow color, compared with healthy control plants. All infected wheat plants were positive when 16S rDNA-amplifying primers were used in nested PCR, contrarily to the healthy ones used as negative controls. Restriction fragment length polymorphism (RFLP) assays conducted on the 16S primed-DNA of BGWLphytoplasma PCR-positive samples yielded a uniform profile and similar to the strain M6 of the BGWLphytoplasma used as an inoculum. This information was further confirmed by sequence analyses. To our knowledge, this is the first report of transmission of BGWL disease to another host, different from Bermuda grass plants, by root-bridge

Keywords

Transmission, Root-bridge system, 16S rDNA, PCR, RFLP