Hastalık Şiddeti, Pektolitik Enzim ve Zearalenone Üretimi Açısından Yerel Mısır Çeşitlerinin Fusarium Verticillioides ´e Karşı Reaksiyonları

Year 2010, Volume: 39 Issue: 1-2-3, 19 - 30, 01.06.2010

Orhan Büyük Nuray Özer

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Reactions of Local Maize Cultivars to Fusarium verticillioides Based on Disease Severity and Production of Pectolytic Enzymes and Zearalenone Toxin

Abstract

Eleven local maize cultivars from the West Black Sea Region of Turkey were analyzed for reactions to Fusarium verticillioides, the causal agent of ear rot in maize. Tests were based on disease severity, associated quantitative and qualitative polygalacturonase (PG) and pectate lyase (PL) activities, and zearalenone (ZEA) concentration after kernel inoculation by pathogen. The kernels of same cultivars were also tested for the presence of ZEA before inoculation. The pathogen caused low disease severity and exhibited low PG and no PL activity in the cultivars Bartın22 and Düzce50. ZEA concentration in all cultivars except two of them were below the recommended limits before inoculation of the pathogen and increased at very low rates in the cultivars Bartın22, Düzce50 and Düzce97 after inoculation. PG and PL activities were positively correlated with disease development. This study suggests the importance of pectolytic enzyme activity produced by F. verticillioides in maize kernels at the early phases of pathogenesis and it can be possible use in monitoring resistance

Keywords

Fusarium verticillioides, cultivar reactions, pectate lyase, polygalacturonase, zearalenone toxin