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Nohutta Ascochyta Yanıklıklık Etmeni Ascochyta Rabıeı'nın TÜRK İzolatlarının Toksin Üretimi ve DNA Sekans Analizleri

Year 2010, Volume: 39 Issue: 1-2-3, 31 - 38, 01.06.2010

Abstract

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Toxin Production and DNA Sequence Analysis of Turkish Isolates of Ascochyta rabiei, the Causual Agent of Ascochyta Blight in Chickpea

Year 2010, Volume: 39 Issue: 1-2-3, 31 - 38, 01.06.2010

Abstract

In this study, twenty isolates of Ascochyta rabiei were isolated from disease-enfecteol chickpea plants which were collected from chickpea growing areas in Turkey. In order to determine solanapyrone production of these isolates, the fungus was grown on Czapek Dox liquid culture medium (CDLCM) for 12 day at two different temperatures. Quantitation of solanapyrones was determined with HPLC analyses. The results demonstrated that all isolates produced solanapyrone A in CDLCM at 200C but not at 300C. Confirmation of the identity of the pathogen was sought by sequence analysis of rDNA. These experiments showed that the sequences of the internal transcribed spacers (ITS) and 5.8 S gene of the seven isolates, which were identical to each other, were also identical to that of a Pakistan isolate of A.rabiei. rDNA sequences of the PCR products of isolates of A.rabiei which were produced different amount of toxin were same

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Details

Primary Language Turkish
Journal Section Articles
Authors

F. Sara Dolar This is me

Publication Date June 1, 2010
Published in Issue Year 2010 Volume: 39 Issue: 1-2-3

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APA Dolar, F. S. (2010). Nohutta Ascochyta Yanıklıklık Etmeni Ascochyta Rabıeı’nın TÜRK İzolatlarının Toksin Üretimi ve DNA Sekans Analizleri. The Journal of Turkish Phytopathology, 39(1-2-3), 31-38.
AMA Dolar FS. Nohutta Ascochyta Yanıklıklık Etmeni Ascochyta Rabıeı’nın TÜRK İzolatlarının Toksin Üretimi ve DNA Sekans Analizleri. The Journal of Turkish Phytopathology. June 2010;39(1-2-3):31-38.
Chicago Dolar, F. Sara. “Nohutta Ascochyta Yanıklıklık Etmeni Ascochyta Rabıeı’nın TÜRK İzolatlarının Toksin Üretimi Ve DNA Sekans Analizleri”. The Journal of Turkish Phytopathology 39, no. 1-2-3 (June 2010): 31-38.
EndNote Dolar FS (June 1, 2010) Nohutta Ascochyta Yanıklıklık Etmeni Ascochyta Rabıeı’nın TÜRK İzolatlarının Toksin Üretimi ve DNA Sekans Analizleri. The Journal of Turkish Phytopathology 39 1-2-3 31–38.
IEEE F. S. Dolar, “Nohutta Ascochyta Yanıklıklık Etmeni Ascochyta Rabıeı’nın TÜRK İzolatlarının Toksin Üretimi ve DNA Sekans Analizleri”, The Journal of Turkish Phytopathology, vol. 39, no. 1-2, pp. 31–38, 2010.
ISNAD Dolar, F. Sara. “Nohutta Ascochyta Yanıklıklık Etmeni Ascochyta Rabıeı’nın TÜRK İzolatlarının Toksin Üretimi Ve DNA Sekans Analizleri”. The Journal of Turkish Phytopathology 39/1-2 (June 2010), 31-38.
JAMA Dolar FS. Nohutta Ascochyta Yanıklıklık Etmeni Ascochyta Rabıeı’nın TÜRK İzolatlarının Toksin Üretimi ve DNA Sekans Analizleri. The Journal of Turkish Phytopathology. 2010;39:31–38.
MLA Dolar, F. Sara. “Nohutta Ascochyta Yanıklıklık Etmeni Ascochyta Rabıeı’nın TÜRK İzolatlarının Toksin Üretimi Ve DNA Sekans Analizleri”. The Journal of Turkish Phytopathology, vol. 39, no. 1-2-3, 2010, pp. 31-38.
Vancouver Dolar FS. Nohutta Ascochyta Yanıklıklık Etmeni Ascochyta Rabıeı’nın TÜRK İzolatlarının Toksin Üretimi ve DNA Sekans Analizleri. The Journal of Turkish Phytopathology. 2010;39(1-2-3):31-8.