Crossability and inheritance of seed coat colour in cowpea at F1 generation

Abstract

A study on crossability and inheritance of seed coat colour in cowpea was conducted between October, 2017 and August, 2018. Two accessions of cowpea: IT98K-205-8 (white seeded) and IT98K-555-1 (brown seeded) were used for the study. Plants were raised in plastic pots in the screen house, and as flowers mature, crosses were performed manually, early in the morning between 7.00 and 9:30 AM. Significant level of differences were detected between the crosses for all traits observed except for number of seeds per pod. Overall, 19 successful pods were generated from 67 crosses representing 28.36% success rate, and indicating a low level of compatibility between accessions. All seeds of F1 plants were black, contradicting the model that maternal parents determine the phenotypes of F1s. However, the two parents bred true for seed coat colour indicating their pure line status. It was shown that maternal effect played significant role regarding crossability, but did not influence the seed coat colour inheritance of the crop. These results indicated epistasis, and it is suggested that the inheritance of seed coat colour in cowpea is polygenic.

Keywords

• Cowpea
• epistatis
• maternal effect
• polygenic
• seed coat colour

References

1. Ajayi, A. T., & Gbadamosi, A. E. (2020). Genetic variability, character association and yield potentials of twenty five accessions of cowpea (Vigna unguiculata L. Walp). Journal of Pure and Applied Agriculture, 5, 1-16.
2. Ajayi, A. T., Gbadamosi, A. E., Olumekun, V. O., & Nwosu, P. O. (2020). GT biplot analysis of shoot traits indicating drought tolerance in cowpea (Vigna unguiculata L. Walp) accessions at vegetative stage. International Journal of Biosciences and Technology, 13(2), 18-33.
3. Ajayi, A. T., Gbadamosi, A. E., & Olumekun, V. O. (2018). Screening for drought tolerance in cowpea (Vigna unguiculata L. Walp) at seedling stage under the screen house condition. International Journal of Biosciences and Technology, 11(1), 1-19.
4. Ajeigbe, H. A., Singh, B. B., & Emechebe, A. M. (2008). Field evaluation of improved cowpea lines for resistance to bacterial blight, virus and striga under infestation in the West African Savannah. African Journal of Biotechnology, 7(20), 3563-3568.
5. Amusa, O. D., Ogunkanmi, A. L., Adetunmbi, J. A., Akinyosoye, S. T., & Ogundipe, O. T. (2019). Morpho-genetic variability in F2 progeny cowpea genotypes tolerant to bruchid (Callosobruchus maculatus). Journal of Agricultural Sciences, 64(1), 53-68.
6. Aremu, C. O., Ariyo, O. J., & Adewale, B. D. (2007). Assessment of selection techniques in genotype x environment interaction in cowpea (Vigna unguiculata L. Walp). African Journal Agriculture Research, 2, 352-355.
7. Carvalho, M., Castro, I., Moutinho-Pereira, J., Correia, C., Egea-Cortines, M., Matos, M., & Lino-Neto, T. (2019). Evaluating stress responses in cowpea under drought stress. Journal of Plant Physiology, 241, 153001.
8. Chandler, V. L., Radicell, J. P., Robbins, T. P., Chen, J., & Turks, D. (1989). Two regulatory genes of the maize anthocyanin pathway are homologous: Isolation of B utilizing R genomic sequences. Plant Cell, 1, 1175-1183.

9. Drabo, I., Smithson, J. B., & Redden, R. (1988). Inheritance of eye pattern and seed coat colour in cowpea (Vigna unguiculata L. Walp). Plant Breeding, 123, 119-123.
10. Egbadzor, K. F., Yeboah, M., Gamedoagbao, D. K., & Offei, S. K. (2014). Inheritance of seed coat colour in cowpea. International Journal of Plant Breeding and Genetics, 8(1), 35-43.
11. FAOSTAT, (2020). Food and agricultural organisation of united nation. http://faostat.fao.org. FAOSTAT_data_8-29-2020-Excel. Last accessed August 29, 2020.
12. Fatokun, C. A., Boukar O, Muranaka S, & Chikoye, D. (2009). Enhancing drought tolerance in cowpea. Proceedings of the 9th African Crop Science Conference, Cape Town, South Africa.
13. Hayatu, M., Shehu, M., & Haruna, H. (2016). Effect of different levels of Striga gesnerioides on the growth and yield of some local and improved cowpea (Vigna unguiculata (L.) Walp) varieties. Bayero Journal of Pure and Applied Sciences, 9(1), 76-81.
14. Herniter, I. A. Lo, R., Muñoz-Amatriaín, M., Lo, S., Guo, Y. N., Huynh, B. L., Mitchell Lucas, M., Jia, Z., Roberts, P. A., Stefano Lonardi, S., & Close, T. J. (2019). Seed coat pattern QTL and development in cowpea (Vigna unguiculata L. Walp). Frontiers in Plant Science, 10, 1-12.
15. Herniter, I. A., Muñoz-amatriaín, M., Lo, S., Guo, Y., & Close, T. J. (2018). Identification of candidate genes controlling black seed coat and pod tip color in cowpea (Vigna unguiculata L. Walp). Genes Genomes Genetics, 8, 3347-3355.
16. Lachyan, T. S., & Dalvi, V. V. (2015). Inheritance study of qualitative and quantitative traits in cowpea (Vigna unguiculata L. Walp). International Journal of Science and Research, 4(4), 2170-2173.
17. Lachyan, T. S., Desai, S. S., & Dalvi, V. V. (2016). Inheritance study of qualitative and quantitative characters in cowpea varieties (Vigna unguiculata L. Walp). Electronic Journal of Plant Breeding, 7, 708-713.
18. Laurentin, H., & Benitez, T. L. (2014). Inheritance of seed coat colour in sesame. Pesquisa Agropecuaria Brasiliera, 49(4), 290-295.
19. Lelou, B., & Van Damme, P. (2000). Production of intraspecific F1 hybrids between wild and cultivated accessions of cowpea (Vigna unguiculata L. Walp) using conventional methods. Communications in Agricultural and Applied Biological Sciences, 71(4), 57-75.
20. Lelou, B., Diatewa, M., & Van Damme, P. (2011). A study of intraspecific hybrid lines derived from the reciprocal crosses between wild accessions and cultivated cowpeas (Vigna unguiculata (L.) Walp). African Journal of Plant Science, 5(6), 337-348.
21. Mohammed, M. S., Russom, Z., & Abdul, S. D. (2010). Studies on crossability between cultivated cowpea (Vigna unguiculata L. Walp) varieties and their wild relative (Var. pubescens TVN110-3 A). International Research Journal of Plant Science, 1(5), 133-135.
22. Mustapha, Y., (2009). Inheritance of seed coat colour in cowpea (Vigna unguiculata L. Walp). Bayero Journal of Pure and Applied Sciences, 2(2), 70-74.
23. Noubissie Tchiagam, J., Bell, J. M., Guissaibirwe, S., & Youmbi, E. (2010). Varietal response of cowpea (Vigna unguiculata (L.) Walp) to Striga gesnerioides (Willd) Vatke race SG5 infestation. Notulae Botanicae Horti–Agrobotanici Cluj–Napoca, 38(2), 33-41.
24. Nwofia, G. E., (2014). Inheritance of leaf shape, pod shape, pod colour and seed coat colour in cowpea (Vigna unguiculata L. Walp). World Journal of Agricultural Sciences, 10(4), 178-184.
25. Ogunkanmi, A. L., & Adekoya, K. (2013). Breeding for long pod trait in cowpea (Vigna unguiculata L. Walp). Journal of Scientific Research and Development, 14, 9-14.
26. Oluwatosin, O. B. (2000). Inheritance of genes controlling seed coat color in cowpea (Vigna unguiculata L. Walp). Journal of Genetics and Breeding, 54, 259-262.
27. Rangkham, T., & Khanna, V. K. (2018). Studies on hybridization and genetic diversity in cowpea (Vigna unguiculata L.). Open Access Journal of Oncology and Medicine, 2(1), 1-10.
28. Singh, B. B., Mai-Kodomi, Y., & Terao, T. (1999). A simple screening method for drought tolerance in cowpea. Indian Journal of Genetics, 59, 211-220.
29. Singh, B. B., Olufajo, O. O., Ishiyaku, M. F., Adeleke, R. A., Ajeigbe, H. A., & Mohammed, S. G. (2006). Registration of six improved germplasm lines of cowpea with combined resistance to Striga genestrioides and Alectra vogelili. Crop Science, 46, 2332-2333.
30. Stoilova, T., & Pereira, G. (2013). Assessment of the genetic diversity in a germplasm collection of cowpea (Vigna unguiculata (L.) Walp.) using morphological traits. African Journal of Agricultural Research, 8, 208-215.
31. Win, K. T., Oo, A. Z., New, K. L., Theon, M. S., & Yukata, H. (2015). Diversity of Myanmar cowpea accessions through seed storage polypeptides and its cross compatibility with the subgenus Ceratotropis. Journal of Soil Science, 3(7), 115-122.
32. Yilwa, V. (2012). Genetics of cowpea: In Inheritance of qualitative and quantitative traits, (pp. 1-228). Lambert Academic Publishing, Riga.