Developed biomaterials for use in three dimensional cultures of liver cells and organoids

Abstract

The 3D bioprinting technique, which is an interdisciplinary study on which basic sciences such as biology and chemistry, especially tissue engineering and bioengineering studies, has recently focused on, is one of the most innovative technologies. 3D bioprinting is an emerging technology with various applications in the construction of tissues and organs that can biologically mimic injured or diseased tissues and organs (biomimetic). In this method, cells, growth factors and biomaterials are combined and a hybrid biomaterial is obtained. By means of biomaterials, cell scaffolds of desired shape, quantity and function can be produced layer by layer with living cells. Creating tissue scaffolds with bioprinting technique is a very important approach, especially to create complex tissues such as liver. Biolinks made from polymers of both natural and synthetic origin have the advantage of applying pressure to soft tissues such as the liver. In this review, studies on hepatocytes were examined and compiled.

Keywords

• 3 dimensional (3D) bioprinting
• biomaterials
• liver cells
• organoids
• tissue engineering

Karaciğer hücreleri ve organoidlerin üç boyutlu kültürlerinde kullanılmak üzere geliştirilmiş biyomalzemeler

Abstract

Doku mühendisliği ve biyomühendislik çalışmaları başta olmak üzere biyoloji ve kimya gibi temel bilimlerin son zamanlarda üzerinde odaklandığı disiplinlerarası bir çalışma olan 3 boyutlu (3B) biyobasım tekniği en yenilikçi teknolojilerden biridir. 3 boyutlu biyobasım, yaralı veya hastalıklı doku ve organları biyolojik olarak taklit edebilecek (biomimetik) doku ve organların yapımında çeşitli uygulamalara sahip gelişmekte olan bir teknolojidir. Bu yöntemde hücreler, büyüme faktörleri ve biyomalzemeler birleştirilir ve hibrit bir biyomalzeme elde edilir. Biyomalzemeler vasıtasıyla canlı hücreler ile katman katman, istenilen şekil, miktar ve fonksiyonda hücre iskeletleri (scaffold) üretilebilir. Özellikle karaciğer gibi karmaşık dokuları oluşturmak için biyobasım tekniği ile doku iskeleleri oluşturmak oldukça önemli bir yaklaşımdır. Hem doğal hem de sentetik kökenli polimerlerden yapılan biyo bağlantılar, karaciğer gibi yumuşak dokulara baskı uygulanması noktasında avantaja sahiptir. Bu derlemede özellikle hepatositler üzerine yapılan çalışmalar incelenmiş ve derlenmiştir.

Keywords

• 3 boyutlu (3B) biyobasım
• biyomalzemeler
• doku mühendisliği
• karaciğer hücreleri
• organoidler

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