A rapid alert system for methicillin resistant Staphylococcus aureus (MRSA) infection

Abstract

This study was designed on the detection time of MRSA at different contamination levels and in this study, a rapid alert system was established for the detection of MRSA, which is among the opportunistic pathogen nosocomial infections. MRSA detection was realized in broths with high and moderate growth at the end of eight hours. However, MRSA detection in broths with growth at all densities was performed after ten hours. After 24 hours of the experiment, colors of the Eppendorf’s were completely changed. In the study, qualitative and quantitative results were achieved with this detection system. It was observed that the RGB sensor was lit in green for the positive result in the qualitative result detection, while the sensor was lit red for the negative result. The quantitative data of the results were monitored through the Seriport screen of the Arduino Program. The MRSA detection of the system designed in the study was accepted as a fast, specific, consistent, cost-effective and non-experienced technical staff required system that can be used as a screening test before the PCR test is performed.

Keywords

• Arduino Program
• biosensor
• methicillin resistant Staphylococcus aureus (MRSA)
• rapid detection method

Metisilin dirençli Staphylococcus aureus (MRSA) enfeksiyonu için hızlı uyarı sistemi

Abstract

Bu çalışma, farklı kontaminasyon seviyelerindeki MRSA’nın tespit süresi üzerine tasarlanmıştır ve çalışmada fırsatçı patojen hastane enfeksiyonları arasında yer alan MRSA tespitine yönelik hızlı bir tespit sistemi oluşturulmuştur. MRSA tespiti yüksek ve orta yoğunluklu üremenin olduğu sıvı besiyerlerinde sekiz saatin sonunda gerçekleştirilmiştir. Tüm yoğunluklardaki üremenin olduğu sıvı besiyerlerindeki MRSA tespiti ise on saatin sonunda gerçekleştirilmiştir. Eppendorf’lar deneyden 24 saat sonra tamamen renk değiştirmiştir. Çalışmada kalitatif ve kantitatif sonuçlara bu tespit sistemiyle ulaşılmıştır. RGB sensörü kalitatif sonuç tespitinde pozitif sonuçta yeşil yanarken negatif sonuçta ise sensörün kırmızı yandığı gözlemlenmiştir. Sonuçların kantitatif verileri Arduino Programı’nın Seriport ekranı aracılığıyla izlenmiştir. Çalışmada tasarısı yapılan sistemin MRSA tespiti hızlı, spesifik, tutarlı, düşük maliyetli, tecrübeli teknik eleman gerektirmeyen ve aynı zamanda PCR testi yapılmadan önce tarama testi olarak kullanılabilecek bir sistem olarak kabul edilmiştir.

Keywords

• Arduino Programı
• biyosensör
• hızlı tespit yöntemi
• metisilin dirençli Staphylococcus aureus (MRSA)

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