EFFECTS OF SOME HEAVY METALS (Pb++, Cu++, Cd++and Hg++) ON CYTOKININ CONTENT IN BEAN (Phaseolus vulgaris cv. Strike) SEEDLINGS

Abstract

In this study, bean seedlings (Phaseolus vulgaris cv. Strike) were investigated the effects of Hg++, Cd++, Cu++ and Pb++ on cytokinin levels. Also the chlorine salts of each four heavy metals were used. Heavy metal solution prepared with pure water. The stock solution of lead was prepared at a concentration of 1.5, 2.0 and 2.5 mM (PbCl2) and other stock solution had a concentration of 0.1, 0.2 and 0.3 mM for copper (CuCl2); 0.05, 0.06, and 0.08 mM for cadmium (CdCl2.H2O); 0.02, 0.04 and 0.06mM for mercury (HgCl2). According the results we have obtained, four heavy metals caused decreases at significant rations, in the endogen cytokinin levels of seedlings. It was found that the decreases in the cytokinin content related with kind of the heavy metal and concentrations. The highest effect on cytokinin was determined in seedlings exposed to mercury. This metal followed the order Cd++ > Cu++ > Pb++. The result were applied to statistically analyze at significant levels (p<0.05 and p<0.01).

Keywords

• Heavy Metal
• Bean Seedling
• Cytokinin

FASULYE FİDELERİNDEKİ (Phaseolus vulgaris cv. Strike) SİTOKİNİN İÇERİĞİ ÜZERİNE BAZI AĞIR METALLERİN (Hg++, Cd++, Cu++ ve Pb++) ETKİLERİ

Öz

Bu çalışmada fasulye fidelerinin (Phaseolus vulgaris cv. Strike) sitokinin miktarları üzerine civa, bakır, kadmiyum ve kurşunun etkileri araştırıldı. Her dört metalinde klor tuzu kullanıldı. Ağır metal solusyonları saf suyla hazırlandı. Civanın stok solusyonundan (HgCl₂) 0.02, 0.04, 0.06 mM, kadmiyumdan (CdCl₂.H₂O) 0.05, 0.06 ve 0.08 mM, bakırdan (CuCl₂) 0.1, 0.2 ve 0.3 mM, kurşundan (PbCl₂) ise 1.5, 2.0 ve 2.5 mM konsantrasyonları hazırlandı. Elde ettiğimiz sonuçlara göre dört ağır metal fidelerin sitokinin seviyesinde önemli oranlarda azalışlar meydana getirdi. Sitokinin içeriğindeki azalışın ağır metalin çeşidi ve konsantrasyonuyla ilişkili olduğu görüldü. Fidelerin içsel sitokinin seviyesi üzerine en fazla toksik etkiyi civa gösterdi, bu metali sırayla kadmiyum, bakır ve kurşun izledi (Cd⁺⁺ > Cu⁺⁺ > Pb⁺⁺). Sonuçlar p<0.05 ve p<0.01 önem seviyelerinde istatistik analize tabi tutuldu.

Anahtar Kelimeler

• Ağır Metal
• Fasulye Fidesi
• Sitokinin

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