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Impotance of polymorphisms of the MIF gene at acute leukemia and its effect on febrile neutropenic attacks

Year 2009, Volume: 15 Issue: 2, 5 - 9, 01.03.2009

Abstract

The objective of this study is to research polymorphisms in MIF ' gene -173 site in variable haemotological malignities and evaluate its effects on febrile neutropenic attacks after chemotherapy in acute leukemia. Single nucleotide polymorphisms (SNPs) at -173 site of the MIF gene was analysed with the PCR-RFLP method (restriction endonudease Alu I) with isolated DNA samples from 48 patients who were treated for ALL and AML and 53 healthy controls, and G and C allel frequencies were compared statistically between patients and healthy controls groups. As a result of the analyzis, it was observed that the rate of patients with GG genotypes was 70.9%, with GC genotypes 29.1%, and with CC genotypes 0%.,Association is evaluated between MIF pozitivities in acute leukemias and distribution of infections, its factors, duration of feveraldays and neutropenia. Within the GC allel group; no significant differences were observed (p>0.05) in infection factors, duration of neutropenia (

References

  • 1. Denkinger CM, Metz C, Fingerle-Rowson G, Denkinger MD, Forsthuber T. Macrophage migration inhibitory factor and its role in autoimmune diseases. Arch Immunol Ther Ex. 2004;52:389-400
  • 2. Bloom BR, Bennett B. Mechanism of a reaction in vitro associated with delayed-type hypersensitivity
  • Science. 1966; 153:80-82
  • 3. Weiser WY, Temple PA, Witek-Giannotti JS, Remold HG, Clark SC, David JR., et al. Molecular cloning of a cDNA encoding a human macrophage migration inhibitory factor. Proc Nat Acad Sci Immunol. 1989;86:7522-7526
  • 4. Paralkar V, Wistow G. Cloning the human gene for macrophage migration inhibitory factor (MIF). Genomics
  • 1994;19:48-51
  • 5. Zhong XB, Leng L, Beitin A, Chen R, McDonald C, Hsiao B, et al. Simultaneous detection of microsatellite repeats and SNPs in the macrophage migration inhibitory factor gene by thin-film biosensor chips and application to rural field studies. Nucleic Acid Res. 2005;33,e121
  • 6. Hizawa N, Yamaguchi E, Takahashi D, Nishihira J, Nishimura M, et al. Functional polymorphisms in the promoter region of macrophage migration inhibitory factor and atopy. Am J Respir Crit Care Med
  • 2004;169:1014-1018
  • 7. De Benedetti F, Meazza C, Vivarelli M, Rossi F, Pistorio A, Lamb R, et al. Functional and prognostic relevance of the -173 polymorphism of the macrophage migration inhibitory factor gene in systemic-onset juvenile idiopathic arthritis. Arthritis Rheu. 2003;48:1398-1407
  • 8. Bernhagen J, Calandra T, Mitchell RA, Martin SB, Tracey KJ, Voelter W, et al. MIF is a pituitary-derived cytokine that potentiates lethal endotoxaemia. Nature
  • 1993;365:756-759
  • 9. Bucala R. MIF rediscovered: cytokine, pituitary hormone, and glucocorticoid-induced regulator of the immune response. Faseb J. 1996; 10:1607-1613
  • 10. Donn RP, Shelley E, Oilier WER. A novel 5-prime- flanking region polymorphism of macrophage migration inhibitory factor is associated with systemic-onset juvenile idiopathic arthritis. Arthritis Rheum. 2001;44:1782-1785
  • 11. Sambrook JJ, Fritsch EF, and Maniatis T. Molecular Clonning: A laboratory manual. 1989; 2nd Edition ,Cold Spring Laboratory Pres, Cold Spring Harbor
  • 12. Bozza FA, Gomes RN, Japiassü AM, Soares M, Castro- Faria-Neto HC, Bozza PT. et al. Macrophage migration inhibitory factor levels correlate with fatal outcome in sepsis. Shock. 2004;22(4):309-13
  • 13. de Mendonça-Filho HT, Gomes GS, Nogueira PM, Fernandes MA, Tura BR, Santos M, et al. Macrophage migration inhibitory factor is associated with positive cultures in patients with sepsis after cardiac surgery
  • Shock. 2005;24:313-317
  • 14. Afig B, Arif RÖ, Zülal Ü. Association of macrophage migration inhibitory factor gene -173G/C polymorphism with prognosis in turkish children with juvenile rheumatoid arthritis. Rheumatol In. 2005;10:296-305
  • 15. Shun CT, Lin JT, Huang SP, Un MT, Wu MS. Expression of macrophage migration inhibitory factor is associated with enhanced angiogenesis and advanced stage in gastric carcinomas. World J Gastroenterol. 2005; 1: 3767-377
  • 16. Vivarelli M, D'Urbano LE, Stringini G, Ghiggeri GM, Caridi G, Donn R, et al. Association of the macrophage migration inhibitory factor -173*C allele with childhood nephrotic syndrome. Pediatr Nephrol. 2008;23:743-748
  • 17. Rigante D, Flex A, Federico G, Pola R, Candelli M, Manna R, et al. Serum macrophage migration inhibitory factor (MIF) in the intercritical phase of hereditary periodic fevers and its relationship with the MIF-173G/C polymorphism. Scand J Rheumatol. 2007;36:307-310
  • 18. Awandare GA, Ouma C, Keller CC, Were T, Otieno R, Ouma Y, et al. A macrophage migration inhibitory factor promoter polymorphism is associated with high-density parasitemia in children with malaria. Genes Immun. 2000;7:568-575.

Akut lösemilerde MIF Genindeki polimorfizmlerin önemi ve febril nötropenîk ataklara etkisi

Year 2009, Volume: 15 Issue: 2, 5 - 9, 01.03.2009

Abstract

Bu çalışmanın amacı; Akut Lösemiler (AL)'de Makrofaj Migrasyon inhibitor Faktörü (MIF) geninin -173 bölgesine ait polimorfizmleri araştırmak ve kemoterapi sonrası gelişen febril nötropenik (FN) ataklara etkisini değerlendirmektir. Akut Myeloblastik Lösemi (AML) ve Akut Lenfoid Lösemi (ALL) tanısıyla tedavi gören 48 hasta ile 53 sağlıklı kontrolden izole edilen DNA örneklerinde MIF geni -173 bölgesine ait tek nükleotid polimorfizmleri, PCR-RFLP yöntemi ile araştırılarak, G ye C allel ve genotip dağılımları hasta ile kontrol grupları arasında karşılaştırmıştır. Analizler sonucunda GG genotipini taşıyan hastaların oranı %70.9, GC'yi taşıyanların oranı %29.1 ye CC'yi taşıyanların oranı %0 olarak saptanmıştır. Hasta ve kontrol grubunda genotip ye allel frekansları açısından anlamlı bir farklılık ile Hardy-Weinberg dengesinden bir sapma gözlenmemiştir. AL'lerde elde edilen MIF pozitifliği ile infeksiyonların dağılımı,'etkenleri, ateşli gün süresi ve nötropeni süresi arasındaki ilişki değerlendirildiğinde; GC allel grubunda, infeksiyonların etkenleri, nötropeni (0.05). Sonuç olarak; Fungal - pnömoni, GC aleli taşıyan grupta, GG aleli taşıyan gruba göre (%22/ll,5) daha sık izlenmiş olup çalışma grubunun' genişletilerek tekrarlanması durumunda anlamlı bir ilişki saptanabileceği düşünülmüştür.

References

  • 1. Denkinger CM, Metz C, Fingerle-Rowson G, Denkinger MD, Forsthuber T. Macrophage migration inhibitory factor and its role in autoimmune diseases. Arch Immunol Ther Ex. 2004;52:389-400
  • 2. Bloom BR, Bennett B. Mechanism of a reaction in vitro associated with delayed-type hypersensitivity
  • Science. 1966; 153:80-82
  • 3. Weiser WY, Temple PA, Witek-Giannotti JS, Remold HG, Clark SC, David JR., et al. Molecular cloning of a cDNA encoding a human macrophage migration inhibitory factor. Proc Nat Acad Sci Immunol. 1989;86:7522-7526
  • 4. Paralkar V, Wistow G. Cloning the human gene for macrophage migration inhibitory factor (MIF). Genomics
  • 1994;19:48-51
  • 5. Zhong XB, Leng L, Beitin A, Chen R, McDonald C, Hsiao B, et al. Simultaneous detection of microsatellite repeats and SNPs in the macrophage migration inhibitory factor gene by thin-film biosensor chips and application to rural field studies. Nucleic Acid Res. 2005;33,e121
  • 6. Hizawa N, Yamaguchi E, Takahashi D, Nishihira J, Nishimura M, et al. Functional polymorphisms in the promoter region of macrophage migration inhibitory factor and atopy. Am J Respir Crit Care Med
  • 2004;169:1014-1018
  • 7. De Benedetti F, Meazza C, Vivarelli M, Rossi F, Pistorio A, Lamb R, et al. Functional and prognostic relevance of the -173 polymorphism of the macrophage migration inhibitory factor gene in systemic-onset juvenile idiopathic arthritis. Arthritis Rheu. 2003;48:1398-1407
  • 8. Bernhagen J, Calandra T, Mitchell RA, Martin SB, Tracey KJ, Voelter W, et al. MIF is a pituitary-derived cytokine that potentiates lethal endotoxaemia. Nature
  • 1993;365:756-759
  • 9. Bucala R. MIF rediscovered: cytokine, pituitary hormone, and glucocorticoid-induced regulator of the immune response. Faseb J. 1996; 10:1607-1613
  • 10. Donn RP, Shelley E, Oilier WER. A novel 5-prime- flanking region polymorphism of macrophage migration inhibitory factor is associated with systemic-onset juvenile idiopathic arthritis. Arthritis Rheum. 2001;44:1782-1785
  • 11. Sambrook JJ, Fritsch EF, and Maniatis T. Molecular Clonning: A laboratory manual. 1989; 2nd Edition ,Cold Spring Laboratory Pres, Cold Spring Harbor
  • 12. Bozza FA, Gomes RN, Japiassü AM, Soares M, Castro- Faria-Neto HC, Bozza PT. et al. Macrophage migration inhibitory factor levels correlate with fatal outcome in sepsis. Shock. 2004;22(4):309-13
  • 13. de Mendonça-Filho HT, Gomes GS, Nogueira PM, Fernandes MA, Tura BR, Santos M, et al. Macrophage migration inhibitory factor is associated with positive cultures in patients with sepsis after cardiac surgery
  • Shock. 2005;24:313-317
  • 14. Afig B, Arif RÖ, Zülal Ü. Association of macrophage migration inhibitory factor gene -173G/C polymorphism with prognosis in turkish children with juvenile rheumatoid arthritis. Rheumatol In. 2005;10:296-305
  • 15. Shun CT, Lin JT, Huang SP, Un MT, Wu MS. Expression of macrophage migration inhibitory factor is associated with enhanced angiogenesis and advanced stage in gastric carcinomas. World J Gastroenterol. 2005; 1: 3767-377
  • 16. Vivarelli M, D'Urbano LE, Stringini G, Ghiggeri GM, Caridi G, Donn R, et al. Association of the macrophage migration inhibitory factor -173*C allele with childhood nephrotic syndrome. Pediatr Nephrol. 2008;23:743-748
  • 17. Rigante D, Flex A, Federico G, Pola R, Candelli M, Manna R, et al. Serum macrophage migration inhibitory factor (MIF) in the intercritical phase of hereditary periodic fevers and its relationship with the MIF-173G/C polymorphism. Scand J Rheumatol. 2007;36:307-310
  • 18. Awandare GA, Ouma C, Keller CC, Were T, Otieno R, Ouma Y, et al. A macrophage migration inhibitory factor promoter polymorphism is associated with high-density parasitemia in children with malaria. Genes Immun. 2000;7:568-575.
There are 23 citations in total.

Details

Primary Language Turkish
Journal Section Articles
Authors

Sebahat Güven This is me

Mustafa Pehlivan This is me

Mehmet Yılmaz This is me

Vahap Okan This is me

Sacide Pehlivan This is me

Publication Date March 1, 2009
Published in Issue Year 2009 Volume: 15 Issue: 2

Cite

APA Güven, S., Pehlivan, M., Yılmaz, M., Okan, V., et al. (2009). Akut lösemilerde MIF Genindeki polimorfizmlerin önemi ve febril nötropenîk ataklara etkisi. Gaziantep Medical Journal, 15(2), 5-9.
AMA Güven S, Pehlivan M, Yılmaz M, Okan V, Pehlivan S. Akut lösemilerde MIF Genindeki polimorfizmlerin önemi ve febril nötropenîk ataklara etkisi. Gaziantep Medical Journal. March 2009;15(2):5-9.
Chicago Güven, Sebahat, Mustafa Pehlivan, Mehmet Yılmaz, Vahap Okan, and Sacide Pehlivan. “Akut lösemilerde MIF Genindeki Polimorfizmlerin önemi Ve Febril nötropenîk Ataklara Etkisi”. Gaziantep Medical Journal 15, no. 2 (March 2009): 5-9.
EndNote Güven S, Pehlivan M, Yılmaz M, Okan V, Pehlivan S (March 1, 2009) Akut lösemilerde MIF Genindeki polimorfizmlerin önemi ve febril nötropenîk ataklara etkisi. Gaziantep Medical Journal 15 2 5–9.
IEEE S. Güven, M. Pehlivan, M. Yılmaz, V. Okan, and S. Pehlivan, “Akut lösemilerde MIF Genindeki polimorfizmlerin önemi ve febril nötropenîk ataklara etkisi”, Gaziantep Medical Journal, vol. 15, no. 2, pp. 5–9, 2009.
ISNAD Güven, Sebahat et al. “Akut lösemilerde MIF Genindeki Polimorfizmlerin önemi Ve Febril nötropenîk Ataklara Etkisi”. Gaziantep Medical Journal 15/2 (March 2009), 5-9.
JAMA Güven S, Pehlivan M, Yılmaz M, Okan V, Pehlivan S. Akut lösemilerde MIF Genindeki polimorfizmlerin önemi ve febril nötropenîk ataklara etkisi. Gaziantep Medical Journal. 2009;15:5–9.
MLA Güven, Sebahat et al. “Akut lösemilerde MIF Genindeki Polimorfizmlerin önemi Ve Febril nötropenîk Ataklara Etkisi”. Gaziantep Medical Journal, vol. 15, no. 2, 2009, pp. 5-9.
Vancouver Güven S, Pehlivan M, Yılmaz M, Okan V, Pehlivan S. Akut lösemilerde MIF Genindeki polimorfizmlerin önemi ve febril nötropenîk ataklara etkisi. Gaziantep Medical Journal. 2009;15(2):5-9.