Miyeloperoksidaz geni G-463A polimorfizm ile romatoid artrit arasında bir ilişki yoktur

Year 2009, Volume: 15 Issue: 3, 14 - 16, 01.04.2009

Sacide Pehlivan Ali Aydeniz Tuğçe Sever Özlem Altındağ Sibel Oğuzkan-nalcı Türkan Harunlar

Abstract

Myeloperoxidase (MPO) has been involved in the pathogenesis of several diseases such as rheumatoid arthritis (RA) through excessive production of reactive oxygen species (ROS) as well as through its genetic polymorphism. We examined whether G-463A polymorphism of Myeloperoxidase (MPO) gene was associated with RA. Exactly, 75 patients'with RA and 90 healthy control subjects were included in this study. The genotyping was determined by polymerase chain reaction-restriction fragment length polymorphism method. The association between these single nucleotide polymorphism (SNP) and RA was analyzed using chi-square test and de-Finetti program. Genotype distributions and allele frequency of RA patients were not significantly different from healthy controls. In addition, it was also determined that there.was no deviation from Hardy-Weinberg Equilibrium in any groups (p>0.05). Whether there was an association between MPO gene G-463A gene polymorphism and RA was investigated for the first time in this study in literature and it was demonstrated that it did not exist in the Turkish- RA patients. It'was planned to investigate the other polymorphisms of MPO gene in the future.

Keywords

Peroxidase, Polymorphism, Genetic, Arthritis, Rheumatoid, Polymorphism, Restriction Fragment Length, Polymerase Chain Reaction

No association between myeloperoxidase gene G-463A polymorphism and rheumatoid arthritis

Abstract

Miyeloperoksidaz (MPO) ve genetik polimorfizmleri Romatoid Artrit (RA) gibi reaktif oksijen türlerinin aşırı arttığı çeşitli hastalıkların patogenezinde rol oynamaktadır. Bu çalışmada MPO G463A polimorfizminin RA ile ilişkisinin olup olmadığı araştırıldı. Çalışma kapsamında 75 hasta ve 90 sağlıklı kontrol birey analiz edildi. Genotiplendirme polimeraz zincir reaksiyonu ve restriksiyon enzimi uzunluk polimorfizmi (PCR-RFLP) yöntemi ile yapıldı. Bu tek nükleotid polimorfizmi (SNP) ve RA arasındaki ilişki ki-kare testi ve de-finetti programları kullanılarak analiz edildi. Kontrol grubu ve RA hasta grubu arasında genotip dağılımı ve allel sıklıkları bakımından istatistiksel olarak anlamlı bir fark yoktu. Bunun yanı sıra gruplarda "Hardy-Weinberg Dengesi"nden sapmanın olmadığı saptandı (p>0.05). MPO geni G-463A polimorfizmi ile RA arasındaki ilişkinin ilk defa analiz edildiği bu çalışma sonucunda Türk RA hastalarında bu ilişkinin olmadığı sonucuna varıldı. Bir sonraki aşamada MPO genindeki diğer polimorfizmlerin çalışılması planlanmaktadır.

Keywords

Peroksidaz, Polimorfizm, genetik, Artrit, romatoid, Polimorfizm, kesim parçacığı uzunluk, Polimeraz zincir reaksiyonu

References

• l.Kochi Y, Suzuki A, Yamada R, Yamamoto K. Genetics of rheumatoid arthritis: Underlying evidence of ethnic differences. J Autoimmun. 2009;32:158-62
• 2.Plenge RM. Recent progres in rheumatoid arthritis genetics: one step towards improved patient care. Curr Opin Rheumatol. 2009;21:262-71
• 3.Pratt AG, Isaacs JD, Mattey DL. Current concepts in the pathogenesis of early rheumatoid arthritis. Best Prac Res Clih Rheumatol. 2009;23:37-48
• 4.Coenen MJ, Gregersen PK. Rheumatoid arthritis: a wiew of the current genetic landscape. Genes Immun. 2009;10:101-ll
• 5.Cascorbi I, Henning S, Brockmöller J, Gephart JMeisel C, Müler JM, Loddenkemper R. Subtantially reduced risk of cancer of the aerodigestive tract in subjects with variant -463A of the myeloperoxidase gene. Cancer Res. 2000;60:644-49
• 6.Hoy A, Tregouet D, Leininger-Muller B, Poirier 0, Maurice M, Sass C, et al. Serum myeloperoxidase concentration in a healthy population: biological variations, familial resemblance and new genetic polymorphisms. Eur J Hum Genet. 2001;9:780-6
• 7.Baskol G, Demir H, Baskol M, Kilic E, Ateş F, Karakukçu Ç, et al. Investigation of protein oxidation and lipid peroxidation in patients with rheumatoid arthritis. Cell Biochem Funct. 2006;24:307-ll
• 8.Miller SA, Dykes DD, Polesky HF. A simple salting out procedure for extracting DNA from nucleated cells. Nucleic Acids Res. 1988; 16:1215
• 9.Minota S, Horie S, Yamada A, Iwamoto M, Yoshio T, Mimori A, et al. Circulating myeloperoxidase and anti-myeloperoxidase antibody in patients with vasculitis. Scand J Rheumatol. 1999;28:94-9
• lO.He C, Tamimi RM, Hankinson SE, Hunter DJ, Han J. A prospective study of genetic polymorphism in MPO, antioxidant status, and breast cancer risk. Breast Cancer Res Treat. 2009; 113:585-94
• ll.Polonikov AV, Solodilova MA, Ivanov VP. Genetic variation of myeloperoxidase gene contributes to atopic asthma susceptibility: a preliminary association study in Russian population. J Asthma. 2009;46:523-8
• 12.Atzeni F, Boiardi L, Casali B, Farnetti E, Sarzi-Puttini P, Pipitone N, et al. Lack of association of the -463 G/A myeloperoxidase promoter polymorphism with Behcet's disease in Italian patients Rheumatology. 2007;46:1547-50