ANTIOXIDANT, ANTIMICROBIAL AND ANTIBIOFILM ACTIVITY OF LEAF EXTRACTS OF WALNUT

Abstract

In this study, the antibiofilm activity of water, ethyl acetate and methanol extracts of walnut (J. regia) leaves were investigated. Antimicrobial and antibiofilm activity of the extracts were investigated against P. aeruginosa, K. pneumoniae, A. hydrophila, S. enterica, S. warneri, S. capitis, S. aureus, E. faecium, E. faecalis, L. monocytogenes Serovar I, L. monocytogenes 19111, L. monocytogenes 4b 19115, L. ivanovii 19119, L. innocua 6a 33090, S. mutans, S. anginosus, B. cereus, B. safensis and B. mojavensis. It was determined that all extracts did not show antimicrobial activity on S. mutans (1a103), S. mutans (2a7) and S. aureus (GMC7), but showed different effects on other bacteria. It was determined that water extract had an antibiofilm effect up to 99.20%, methanol extract 95.05% and ethyl acetate extract 95.22%. It has been determined that the extracts are more effective in preventing biofilm formation than removing the formed biofilm. In addition, it was determined that ethyl acetate activity had the highest antioxidant activity.

Keywords

• Antibiofilm
• Juglans regia
• Plant extract
• Antibacterial Effect

Project Number

1803F056

CEVİZ YAPRAK EKSTRELERİNİN ANTİOKSİDAN, ANTİMİKROBİYEL VE ANTİBİYOFİLM AKTİVİTESİ

Abstract

Bu çalışmada ceviz (Juglans regia) yapraklarının su, etil asetat ve metanol ekstrelerinin antibiyofilm aktivitesi araştırılmıştır. Ekstrelerin antimikrobiyel ve antibiyofilm aktivitesi Pseudomonas aeruginosa, Klebsiella pneumoniae, Aeromonas hydrophila ve Salmonella enterica, Staphylococcus warneri, Staphylococcus capitis, Staphylococcus aureus, Enterococcus faecium, E. faecalis, Listeria monocytogenes Serovar I, L. monocytogenes 19111, L. monocytogenes 4b 19115, L. ivanovii 19119, L. innocua 6a 33090, Streptococcus mutans, S. anginosus, Bacillus cereus, Bacillus safensis ve B. mojavensis’a karşı araştırılmıştır. Bütün ekstrelerinin S. mutans (1a103), S. mutans (2a7) ve S. aureus (GMC7) üzerinde antimikrobiyel aktivitesinin olmadığı diğer bakteriler üzerinde ise farklı oranlarda etki gösterdikleri belirlenmiştir. Su ekstresinin %99.20 metanol ekstresinin %95.05 ve etil asetat ekstresinin ise %95.22 oranlarına varan antibiyofilm etkilerinin olduğu tespit edilmiştir. Ekstrelerin biyofilm oluşumunu azaltmada oluşmuş biyofilmi gideriminden daha etkili oldukları tespit edilmiştir. Ayrıca en yüksek antioksidan aktiviteye etil asetat aktivitesinin sahip olduğu belirlenmiştir.

Keywords

• Antibiyofilm
• Juglans regia
• Bitki ekstreleri
• Antibakteriyel etki

Supporting Institution

Eskişehir Teknik Üniversitesi

Project Number

1803F056

Thanks

2211-C Yurtiçi Öncelikli Alanlar Doktora Bursu ile doktora çalışmamı destekleyen TÜBİTAK’a ve tez çalışmamın sürdürülmesini sağlayan BAP proje komisyonuna teşekkürlerimi sunarım

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