BEYAZ PEYNİR MİKROBİYOTASINDA KÜLTÜROMİK VE SHOTGUN METAGENOMİK TEKNOLOJİLERİN DEĞERLENDİRİLMESİ

Abstract

Omik teknolojiler; DNA, RNA, genler, proteinler ve metabolitlerin araştırılması için kullanılan araçlar ve metotlardan oluşan sistematik yöntemler bütünüdür. Son yıllarda mikroorganizmaların tanımlanmasında ve işlevlerinin belirlenmesinde genomik, transkriptomik, proteomik ve metabolomik alanlarda yapılan çalışmalar artış göstermektedir. Genomik ve transkritptomik çalışmalar kapsamında mikroorganizmaların genom dizilerinin belirlenmesinde ve gen ifade analizlerinde yeni nesil dizileme sistemleri ile biyoinformatik araçlar birlikte kullanılmaktadır. Bu çalışmada, beyaz peynirin taşıdığı toplam mikrobiyel yükün oluşturduğu beyaz peynir mikrobiyotasının belirlenmesinde, kültürden bağımsız bir yöntem olan shotgun metagenomik ile kültüre dayalı bir yöntem olan ve mikroorganizmaların tanımlanmasına olanak sağlayan kültüromik metotları üzerinde durulmuştur. Çalışma ile yakın gelecekte beyaz peynir gibi geleneksel gıda ürünlerinin yeni teknikler değerlendirilerek araştırılması gerekliliğinin önemi vurgulanmıştır. Kültüromik, metagenomik gibi yenilikçi teknikler, geleneksel gıda ürünlerinin mikrobiyota tanımlanması üzerinde daha az belirsizlik ile çalışılmasına olanak sağlayabilmektedir.

Keywords

• beyaz peynir
• mikrobiyota
• omik teknolojiler
• metagenomik
• kültüromik
• MALDI-TOF MS

EVALUATION OF CULTUROMICS AND SHOTGUN METAGENOMIC TECHNOLOGIES IN WHITE CHEESE MICROBIOTA

Abstract

Omics technologies are a set of systematic methods consisting of tools and applications used to investigate genes, DNA, RNA, proteins and metabolites. In recent years, genomics, transcriptomics, proteomics and metabolomics have been mainly utilized in the identification and determination of microorganisms. Within the scope of genomic and transcriptomics studies, next generation sequencing systems and bioinformatics tools are used together to determine the genome sequences of microorganisms and gene expression analysis, respectively. In this study, shotgun metagenomics, the culture-independent method, and culture-dependent culturomics approach that allows identification of microorganisms are emphasized for the detection of white cheese microbiota. The study has been emphasized the importance of researching traditional food products such as white cheese by evaluating new techniques in the future. Innovative techniques such as culturomics and metagenomics can enable the study of traditional food products to be studied with less uncertainty on the microbiota identification.

Keywords

• white cheese
• microbiota
• omic technologies
• metagenomics
• culturomics
• MALDI-TOF MS

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