DETERMINATION OF STAPHYLOCOCCUS AUREUS PREVALENCE AND ANTIBIOTIC RESISTANCE PROFILES, ANTIBIOTIC RESISTANCE AND ENTEROTOXIN GENES IN POULTRY MEATS

Abstract

In this study, it was aimed to investigate the prevalence of Staphylococcus aureus in poultry meats (32 chickens, 9 turkeys, 9 quails) sold in Isparta and Antalya provinces, and to determine the presence of enterotoxin genes as well as antibiotic resistance profiles and resistance genes. Baird Parker agar medium was used to isolate presumptive S. aureus from poultry meats. The species-level identification of isolates was done by polymerase chain reaction (PCR) using primer pair specific to the thermostable nuclease gene (nuc) in S. aureus. As a result of the PCR analysis, amplicons of 458 bp specific to the nuc gene were obtained in 16 of 130 presumptive S. aureus isolates. The frequency of S. aureus in poultry meat samples was found to be 20% (10/50). Coagulase test showed that all the S. aureus isolates were coagulase positive. Disk diffusion test showed that all of the isolates (100 %) were susceptible to chloramphenicol and teicoplanin and resistant to penicillin G. It was determined that 81.25 % (13/16) of the isolates were methicillin resistant S. aureus (MRSA). The most common antibiotic resistance gene in S. aureus strains was found to be blaZ (62.5 %, 10/16) as a result of PCR. The presence of sea, seb, sec, sed and see genes was not detected in any of the S. aureus strains.

Keywords

• Poultry meat
• Staphylococcus aureus
• antibiotic resistance
• antibiotic resistance gene
• enterotoxin gene

KANATLI ETLERİNDE STAPHYLOCOCCUS AUREUS YAYGINLIĞI VE ANTİBİYOTİK DİRENÇ PROFİLLERİNİN, ANTİBİYOTİK DİRENÇ VE ENTEROTOKSİN GENLERİNİN BELİRLENMESİ

Abstract

Bu çalışmada, Isparta ve Antalya illerinde perakende satışı yapılan kanatlı etlerinde (32 piliç, 9 hindi, 9 bıldırcın) Staphylococcus aureus yaygınlığının araştırılması, antibiyotik direnç profilleri ve direnç genlerinin yanı sıra enterotoksin genleri varlığının belirlenmesi amaçlanmıştır. Kanatlı etlerinden muhtemel S. aureus izolasyonunda Baird Parker agar besiyeri kullanılmıştır. İzolatların tür düzeyinde tanısı S. aureus’da termostabil nükleaz genine (nuc) özgü primer çifti kullanılarak polimeraz zincir reaksiyonu (PZR) ile yapılmıştır. 130 muhtemel S. aureus izolatının 16’sında nuc genine özgü 458 bç büyüklüğünde amplikonlar elde edilmiştir. Kanatlı eti örneklerinde S. aureus bulunma sıklığı % 20 (10/50) bulunmuştur. Koagülaz testi, S. aureus izolatlarının tamamının koagülaz pozitif olduğunu göstermiştir. Disk difüzyon testi, izolatların tamamının (% 100) kloramfenikole ve teikoplanine duyarlı, penisilin G’ye ise dirençli olduğunu göstermiştir. İzolatların % 81.25’inin (13/16) metisilin dirençli S. aureus (MRSA) olduğu tespit edilmiştir. PZR işlemi sonucu S. aureus suşlarında en sık rastlanan antibiyotik direnç geninin blaZ (% 62.5, 10/16) olduğu tespit edilmiştir. S. aureus suşlarının hiçbirinde sea, seb, sec, sed ve see geni varlığı belirlenmemiştir.

Keywords

• Kanatlı eti
• Staphylococcus aureus
• antibiyotik direnç
• antibiyotik direnç geni
• enterotoksin geni

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