ÇEVRESEL DEĞİŞKENLERİN LAKTİK ASİT BAKTERİLERİ TARAFINDAN ANTİMİKROBİYAL MADDE ÜRETİMİ ÜZERİNDEKİ ETKİSİ: BOX-BEHNKEN TASARIM YAKLAŞIMI

Year 2025, Volume: 50 Issue: 3, 466 - 478, 10.06.2025

Evrim Güneş Altuntaş

https://doi.org/10.15237/gida.GD25018

Abstract

Bu araştırmada, laktik asit bakterileri (LAB) tarafından antimikrobiyal madde üretimi için optimum koşulları belirlemek amacıyla 3 faktörlü Box-Behnken deneysel tasarımı (BBD) kullanılmıştır. Deneysel parametreler üç değişkenden oluşmakta olup, bu değişkenler: sıcaklık (30, 35 ve 37oC), inkübasyon süresi (24, 48 ve 72 saat) ve substrat konsantrasyonu (%1, %2 ve %3) olarak belirlenmiştir. Lactiplantibacillus plantarum F2 izolatı tarafından antimikrobiyal madde üretiminin, değişen inkübasyon süresinden etkilendiği gözlenmiştir. Pediococcus pentosaceus 50 izolatının en yüksek antimikrobiyal madde üretimi (deneysel süreçte elde edilen ortalama inhibisyon zon çapı 12.00 mm; Box-Behnken tasarımında tahmin edilen değer 12.09 mm), inkübasyon sıcaklığı 37°C, inkübasyon süresi 24 saat ve ortamda %2'lik bir substrat konsantrasyonu olduğunda elde edilmiştir. Bu bulgular, LAB'nin antimikrobiyal aktivitesinin genellikle logaritmik fazın sonuna doğru ve durağan fazda arttığını, bunun da muhtemelen ikincil metabolitlerin artan üretiminden kaynaklandığını göstermektedir.

Keywords

Lactic acid bacteria , antimicrobial activity , optimization

Project Number

TÜSEB 12028

IMPACT OF ENVIRONMENTAL VARIABLES ON ANTIMICROBIAL SUBSTANCE PRODUCTION BY LACTIC ACID BACTERIA: A BOX-BEHNKEN DESIGN APPROACH

Abstract

In this research, we employed a 3-factor Box-Behnken experimental design (BBD) to ascertain the optimal conditions for the production of antimicrobial substances by lactic acid bacteria (LAB). The experimental parameters encompassed three variables: temperature (30, 35 and 37oC), incubation time (24, 48 and 72 hours), and substrate concentration (1%, 2% and 3%). The production of antimicrobial substances by Lactiplantibacillus plantarum F2 isolate was influenced by the incubation period. The highest antimicrobial substance production (average inhibition zone diameter 12.00 mm for antimicrobial activity assay; 12.09 mm for Box-Behnken estimation) of Pediococcus pentosaceus 50 isolate was achieved at a temperature of 37°C, an incubation period of 24 hours, and a substrate concentration of 2% in the environment. These findings indicate that the antimicrobial activity of LAB typically intensifies toward the end of the logarithmic phase and into the stationary phase, likely due to the increased production of secondary metabolites.

Keywords

Lactic acid bacteria , antimicrobial activity , optimization

Ethical Statement

Author Contribution Conceptualization: EGA. Analysis and data curation: BS, AAC, OK, EGA. Funding acquisition: EGA. Methodology: OK, EGA. Investigation: BS, AAC, OK. Supervision: EGA. Writing – original draft - review & editing: EGA. All authors have read and agreed to the published version of the manuscript. Conflict of Interest The authors declare no competing interests.

Supporting Institution

This study was supported by TUSEB (Health Ministry of Turkey) with the Grant No #12028.

Project Number

TÜSEB 12028

Thanks

The Lactic acid bacteria isolates used in this study were obtained previously in the project supported by TUBITAK Grant No #119O343 and the master degree thesis of Asena Aslihan Celik submitted to Ankara University Science Institute, Ankara-Turkey.

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