Türkiye’de Antepfıstığından Elde Edilen Alternaria spp.’nin PCR-RFLP ile Karakterizasyonu ve Klonal Soy Hattına İlişkin Bulgular

Abstract

Alternaria spp., antep fıstığında (Pistacia vera) ve diğer birçok üründe önemli verim kayıplarına neden olmaktadır. Bu çalışmada, Türkiye’de yerel antep fıstığı çeşitlerinden elde edilen Alternaria izolatları, ITS, Actin ve Tef1-α bölgelerine dayalı PCR-RFLP yöntemi kullanılarak karakterize edilmiştir. PCR amplifikasyonu sonucunda sırasıyla yaklaşık 600, 250 ve 280 bp büyüklüğünde ürünler elde edilmiştir. Restriksiyon analizi, izolatlar arasında herhangi bir polimorfizm bulunmadığını göstermiş ve bu izolatların tek bir klonal soya ait olabileceğini düşündürmüştür.

Keywords

• Antepfıstığı
• Alternaria
• PCR-RFLP

Supporting Institution

Proje çalışmaları Gaziantep Üniversitesi tarafından desteklenmiştir.

Ethical Statement

Etik beyan gerekli değildir.

Thanks

Safa J. Tuama Ali ve Ali Alayedi Irak hükümeti tarafından, Selçuk Başbuğa TÜBİTAK tarafından desteklenmiştir.

PCR-RFLP Characterization of Alternaria spp. from Pistachio in Türkiye and Evidence for a Putative Clonal Lineage

Abstract

Alternaria spp. cause significant yield losses in pistachio (Pistacia vera) and other crops. In this study, Alternaria isolates collected from local pistachio cultivars in Türkiye were characterized using PCR-RFLP based on ITS, Actin, and Tef1-α regions. PCR amplification generated fragments of approximately 600, 250, and 280 bp, respectively. Restriction analysis revealed no polymorphism among the isolates, suggesting that they may belong to a single clonal lineage.

Keywords

• Pistachio
• Alternaria
• PCR-RFLP

Supporting Institution

This research was supported by Gaziantep University

Ethical Statement

Not applicable to this article.

Thanks

Safa J. Tuama Ali and Ali Alayedi were supported by scholarships provided by the Government of Iraq. Selçuk Başbuğa was supported by a scholarship from TÜBİTAK.

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